IkB-alpha Antibody (E130)

Product Discontinued

IkB-alpha Antibody (E130) Summary

• Immunogen: A synthetic peptide corresponding to human IkappaB-alpha was used as immunogen.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NFKBIA
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:20
  • Western Blot 1:1000-10000
• Application Notes: This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation. In Western blot this antibody detects a band at approximately 35kDa.
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to work with: Bovine, Pig.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 59% PBS, 0.05% BSA and 40% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Tissue culture supernatant

Notes

Produced using Abcam's RabMab® technology. RabMab® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Alternate Names for IkB-alpha Antibody (E130)

• alpha
• I-kappa-B-alpha
• IkappaBalphaNF-kappa-B inhibitor alpha
• IKBAikB-alpha
• IkBalpha
• IkB-alpha
• MAD3
• MAD-3
• Major histocompatibility complex enhancer-binding protein MAD3
• NFKBI
• NFKBIA
• nuclear factor of kappa light chain gene enhancer in B-cells
• nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

Background

The NF-eB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IeB-a protein (1-4). In response to many different NF-eB-inducing agents

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IkB-alpha Antibody (NB110-57109)(2)

Publications using NB110-57109

• Meiler S, Baumer Y, Huang Z et al. Selenoprotein K is required for palmitoylation of CD36 in macrophages: implications in foam cell formation and atherogenesis. J Leukoc Biol 2013-02-26 [PMID: 23444136] (ICC/IF, Human)
• Pierce GL, Lesniewski LA, Lawson BR et al. Nuclear Factor-{kappa}B Activation Contributes to Vascular Endothelial Dysfunction via Oxidative Stress in Overweight/Obese Middle-Aged Older Humans. Circulation;119(9):1284-1292. 2009-01-01 [PMID: 19237660]

Bioinformatics

• Gene Symbol: NFKBIA
• Entrez:
  • 4792(Human)
  • 18035(Mouse)
  • 25493(Rat)
• Uniprot:
  • P25963()