IGSF4B/SynCAM3/CADM3 Antibody [Unconjugated]

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IGSF4B/SynCAM3 was detected in immersion fixed BG01V human embryonic stem cells differentiated into neurons using Goat Anti-Human IGSF4B/SynCAM3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3678) at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

IGSF4B/SynCAM3/CADM3 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human IGSF4B/SynCAM3 isoform 1
Pro21-Tyr329
Accession # Q8N126
Specificity
Detects human IGSF4B/SynCAM3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) IGSF4C is observed and less than 2% cross-reactivity with rhIGSF4, rhIGSF4D, and rhIGSF8 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CADM3
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGSF4B/SynCAM3/CADM3 Antibody [Unconjugated]

  • BIgR
  • Brain immunoglobulin receptor
  • CADM3
  • cell adhesion molecule 3
  • IGSF4B
  • IGSF4BSynCAM3
  • Immunoglobulin superfamily member 4B
  • member 4B
  • Necl-1
  • NECL1TSLC1-like protein 1
  • Nectin-like protein 1
  • Synaptic cell adhesion molecule 3
  • SynCAM3
  • TSLC1-like 1
  • TSLL1
  • TSLL1SYNCAM3

Background

IGSF4B (immunoglobulin superfamily member 4B), also called CADM3, TSLL1, Necl‑1 or SynCAM-3, is a neural tissue-specific member of the nectin-like family of immunoglobulin superfamily (1, 2). It is a 48‑50 kDa type I transmembrane (TM) glycoprotein that is concentrated at non-junctional contact sites of neuronal axons and glial processes (2, 3). In myelinated peripheral nerve fibers, IGSF4B is concentrated at nodes of Ranvier in contact sites of Schwann cells (2). The 398 amino acid (aa) human IGSF4B contains a 24 aa signal sequence, a 306 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 47 aa cytoplasmic domain. The ECD contains an N-terminal V-type Ig-like domain that is responsible for Ca++-independent homophilic and heterophilic interactions with Nectin-1, Nectin-3 or Necl‑2 (IGSF-4) in trans. It also contains two C2-type Ig-like domains that are responsible for Ca++-independent homophilic dimerization in cis that is thought to precede trans interaction (2, 4, 5). The cytoplasmic domain binds members of the MAGUK guanylate kinase subfamily, such as Dlg3, Pals2 and CASK (2). These activities are thought to play roles in adhesion and architecture at the synapse (2). We have also shown that IGSF4B promotes in vitro outgrowth of cortical neurons. Of two known splice variants, one shows an insertion of 34 aa near the mature N-terminus and probably represents a 60 kDa form. A smaller variant shows deletions of aa 185‑231, and 281‑398, which includes the first C2-type Ig-like domain and the TM segment. The IGSF4B ECD is highly conserved, sharing 95‑96% aa identity between human mouse, rat, canine and bovine sequences. The ECD of IGSF4A, B, C and D proteins share 35‑50% aa identity.

  1. Fukuhara, H. et al. (2001) Oncogene 20:5401.
  2. Kakunaga, S. et al. (2005) J. Cell Sci. 118:1267.
  3. Fukami, T. et al. (2003) Gene 323:11.
  4. Shingai, T. et al. (2003) J. Biol. Chem. 278:35421
  5. Dong, X. et al. (2006) J. Biol. Chem. 281:10610.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CADM3
Uniprot