IGSF4B/SynCAM3/CADM3 Antibody [Unconjugated] Summary
Mouse myeloma cell line NS0-derived recombinant human IGSF4B/SynCAM3 isoform 1
Accession # Q8N126
Detects human IGSF4B/SynCAM3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) IGSF4C is observed and less than 2% cross-reactivity with rhIGSF4, rhIGSF4D, and rhIGSF8 is observed.
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- Western Blot 0.1 ug/mL
- Flow Cytometry 2.5 ug/10^6 cells
- Immunocytochemistry 5-15 ug/mL
Packaging, Storage & Formulations
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for IGSF4B/SynCAM3/CADM3 Antibody [Unconjugated]
- Brain immunoglobulin receptor
- cell adhesion molecule 3
- Immunoglobulin superfamily member 4B
- member 4B
- NECL1TSLC1-like protein 1
- Nectin-like protein 1
- Synaptic cell adhesion molecule 3
- TSLC1-like 1
IGSF4B (immunoglobulin superfamily member 4B), also called CADM3, TSLL1, Necl‑1 or SynCAM-3, is a neural tissue-specific member of the nectin-like family of immunoglobulin superfamily (1, 2). It is a 48‑50 kDa type I transmembrane (TM) glycoprotein that is concentrated at non-junctional contact sites of neuronal axons and glial processes (2, 3). In myelinated peripheral nerve fibers, IGSF4B is concentrated at nodes of Ranvier in contact sites of Schwann cells (2). The 398 amino acid (aa) human IGSF4B contains a 24 aa signal sequence, a 306 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 47 aa cytoplasmic domain. The ECD contains an N-terminal V-type Ig-like domain that is responsible for Ca++-independent homophilic and heterophilic interactions with Nectin-1, Nectin-3 or Necl‑2 (IGSF-4) in trans. It also contains two C2-type Ig-like domains that are responsible for Ca++-independent homophilic dimerization in cis that is thought to precede trans interaction (2, 4, 5). The cytoplasmic domain binds members of the MAGUK guanylate kinase subfamily, such as Dlg3, Pals2 and CASK (2). These activities are thought to play roles in adhesion and architecture at the synapse (2). We have also shown that IGSF4B promotes in vitro outgrowth of cortical neurons. Of two known splice variants, one shows an insertion of 34 aa near the mature N-terminus and probably represents a 60 kDa form. A smaller variant shows deletions of aa 185‑231, and 281‑398, which includes the first C2-type Ig-like domain and the TM segment. The IGSF4B ECD is highly conserved, sharing 95‑96% aa identity between human mouse, rat, canine and bovine sequences. The ECD of IGSF4A, B, C and D proteins share 35‑50% aa identity.
- Fukuhara, H. et al. (2001) Oncogene 20:5401.
- Kakunaga, S. et al. (2005) J. Cell Sci. 118:1267.
- Fukami, T. et al. (2003) Gene 323:11.
- Shingai, T. et al. (2003) J. Biol. Chem. 278:35421
- Dong, X. et al. (2006) J. Biol. Chem. 281:10610.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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