IGSF2/CD101 Antibody (307707) [CoraFluor™ 1]

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 307707
• Clonality: Monoclonal
• Host: Rat
• Conjugate: CoraFluor 1

IGSF2/CD101 Antibody (307707) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse IGSF2/CD101
  Gln21-Tyr1033
  Accession # A8E0Y8
• Specificity: Detects mouse IGSF2/CD101.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: CD101
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for IGSF2/CD101 Antibody (307707) [CoraFluor™ 1]

• CD101 antigen
• CD101 molecule
• CD101
• Cell surface glycoprotein V7
• EWI101
• EWI-101
• Glu-Trp-Ile EWI motif-containing protein 101
• IGSF2
• Leukocyte Surface Protein
• member 2
• V7

Background

CD101 is a disulfide-linked homodimeric type 1 glycoprotein. The peptide is comprised of 7 extracellular V-type IgSF domains. CD101 is highly expressed on monocytes, granulocytes, mucosal T cells, and on activated peripheral blood T cells. Expression is weak on resting T and B and NK cells, absent from platelets and weak or absent from most hematopoietic cell lines. CD101 is thought to play a co-stimulatory role in TCR/CD3-mediated T cell activation. Monoclonal antibodies against CD101 inhibit allogeneic T cell responses. Studies suggest that CD101 plays a major role in the activation of T cells by skin dendritic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD101