Rat anti-Mouse IgM Heavy Chain Secondary Antibody [Biotin]

Flow Cytometry: Rat anti-Mouse IgM Heavy Chain Secondary Antibody [Biotin] [NBP1-43527] - Staining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) FITC and 0.25 ug of Rat IgG2a Isotype Control Biotin (left) or 0.25 ug of Anti-Mouse IgM Biotin (right) followed by Streptavidin PE. Total viable cells were used for analysis.

Product Discontinued

Rat anti-Mouse IgM Heavy Chain Secondary Antibody [Biotin] Summary

• Immunogen: Mouse IgM
• Specificity: The eB121-15F9 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated eB121-15F9 can be used as a detection secondary for mouse IgM.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: IGHM
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Reactivity Notes

This antibody is reactive to Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2) with 0.1% gelatin
• Preservative: 0.09% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Rat anti-Mouse IgM Heavy Chain Secondary Antibody [Biotin]

• Constant region of heavy chain of IgM
• Ig Mu Chain C Region BOT
• Ig Mu Chain C Region GAL
• Ig Mu Chain C Region OU
• immunoglobulin heavy constant mu
• Immunoglobulin mu chain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGHM