IgM Antibody (II/41) - BSA Free

Flow Cytometry: IgM Antibody (II/41) [NBP1-43303] - Analysis of PE/Cy7 conjugate of NBP1-43303. Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.06 micrograms of Rat IgG2a ? Isotype Control PE-Cy7 (left) or 0.06 micrograms of Anti-Mouse IgM PE-Cy7 (right). Cells in the ly

Flow Cytometry: IgM Antibody (II/41) [NBP1-43303] - Staining of C57BL/6 splenocytes with 0.5 ug of Rat IgG2a kappa Isotype Control Purified (open histogram) or 0.5 ug of Anti-Mouse IgM Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE. Total viable cells were used for analysis.

Flow Cytometry: IgM Antibody (II/41) [NBP1-43303] - Analysis of Allophycocyanin conjugate of NBP1-43303. Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.125 ug of Rat IgG2a K Isotype Control APC (left) or 0.125 ug of Anti-Mouse IgM APC (right). Cells in the small scatter

Flow Cytometry: IgM Antibody (II/41) [NBP1-43303] - Analysis of Allophycocyanin conjugate of NBP1-43303. Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.125 ug of Rat IgG2a K Isotype Control APC (left) or 0.125 ug of Anti-Mouse IgM APC (right). Cells in the small scatter

Flow Cytometry: IgM Antibody (II/41) [NBP1-43303] - Analysis of Biotin conjugate of NBP1-43303. Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) APC and 0.06 ug of Rat IgG2a kappa Isotype Control Biotin (left) or 0.06 ug of Anti-Mouse IgM Biotin (right) followed by Streptavidin PE .

Flow Cytometry: IgM Antibody (II/41) [NBP1-43303] - Analysis of FITC conjugate of NBP1-43303. Staining of C57BL/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) APC and 0.125 ug of Rat IgG2a K Isotype Control FITC (left) or 0.125 ug of Anti-Mouse IgM FITC (right). Total viable cells were used for ana

Flow Cytometry: IgM Antibody (II/41) [NBP1-43303] - Analysis of PE conjugate of NBP1-43303. The II/41 antibody has been tested by flow cytometric mouse spleen cell suspension. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^

Flow Cytometry: IgM Antibody (II/41) [NBP1-43303] - Analysis of PE/Cy5 conjugate of NBP1-43303. Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC and 0.06 ug of Rat IgG2a Isotype Control PE-Cy5 (left) or 0.06 ug of Anti-Mouse IgM PE-Cy5 (right). Total viable cells were used for

• Reactivity: Mu
• Applications: ELISA, Flow, IHC
• Clone: II/41
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

IgM Antibody (II/41) - BSA Free Summary

• Description: Novus Biologicals Rat IgM Antibody (II/41) - BSA Free (NBP1-43303) is a monoclonal antibody validated for use in IHC, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this antibody was mouse IgM.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: IGHM
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
• Application Notes: The II/41 antibody has been tested by flow cytometric analysis of mouse spleen cell suspension. This can be used at less than or equal to 1 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8cells/test.

Reactivity Notes

This antibody is reactive to Mouse.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for IgM Antibody (II/41) - BSA Free

• AGM1
• DKFZp686I15196
• DKFZp686I15212
• FLJ00385
• IgM
• immunoglobulin heavy constant mu
• MGC104996
• MGC52291
• MU
• VH

Background

The II/41 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGHM