Rabbit IgG Isotype Control [Agarose Immobilized]

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Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Agarose Immobilized

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Catalog# & Conjugate Size Price

Rabbit IgG Isotype Control [Agarose Immobilized] Summary

Description
This Rabbit IgG immunoglobulin is useful as an isotype control for Rabbit IgG antibodies.
Kit Type
Kit
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunoprecipitation 1:10 - 1:500
Application Notes
Rabbit IgG was coupled to agarose beads using a cyanogen bromide method. Rabbit IgG concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG, before coupling to agarose beads. Useful as a positive control in immunoassays. IP control that is used in conjunction with agarose-immobilize antibodies made in rabbit. WB usage was reported in scientific literature (PMID: 24569374). Proximity Ligation Assay usage was reported in scientific literature (PMID: 24569374). Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.
Publications
Read Publications using
NB600-441 in the following applications:

  • PLA
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS.
Preservative
0.1% Sodium Azide
Purity
Immunogen affinity purified

Notes

Protein concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Alternate Names for Rabbit IgG Isotype Control [Agarose Immobilized]

  • IgG
  • immunoglobulin heavy constant gamma 1 (G1m marker)

Background

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG Isotype Control (NB600-441)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: PLA, WB.


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PLA
(1)
WB
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

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Blogs on IgG.

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CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

Understanding the Importance of Human IgG
Human IgG is a component of the immune system that protects the body from infection. It is the most abundantly found antibody isotype within the circulatory system of the human body. All antibody isotypes contain two heavy chains and two light chains...  Read full blog post.

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