Goat IgG Isotype Control [Agarose]

Product Discontinued

Goat IgG Isotype Control [Agarose] Summary

• Description: This Goat IgG immunoglobulin is useful as an isotype control for Goat IgG antibodies.
• Kit Type: Kit
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IGHG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10 - 1:500
• Application Notes: IP: Useful as a positive control in immunoassays. IP control that is used in conjunction with agarose-immobilize antibodies made in goat (15 to 25 ul of gel slurry per 0.1 to 1 mg of protein lysate or extract). Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: 2 - 8C / 2 years from date of receipt
• Buffer: PBS.
• Preservative: 0.09% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Goat IgG concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG, before coupling to agarose beads. Final concentration is 0.1 mg antibody in 0.4 ml of 50% slurry containing 0.2 ml agarose.

Alternate Names for Goat IgG Isotype Control [Agarose]

• IgG
• Immunoglobulin G
• ImmunoglobulinG

Background

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGHG1