Donkey anti-Sheep IgG Secondary Antibody [NL493]


Detection of human AIF by incubating cells with 5 μg/mL of Sheep anti-Human AIF polyclonal antibody (AF5824) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Sheep IgG more

Product Details

Reactivity ShSpecies Glossary
Applications Flow, IHC, ICC/IF

Donkey anti-Sheep IgG Secondary Antibody [NL493] Summary

Additional Information
NorthernLights 493 Fluorochrome-labeled Antibody, X Absorbed
Sheep IgG
Sheep IgG (H+L)
Purity Statement
Antigen Affinity-purified


  • Flow Cytometry
  • Immunocytochemistry
  • Immunohistochemistry
Read Publications using
NL012 in the following applications:

Packaging, Storage & Formulations

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.
Sodium Azide


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Donkey anti-Sheep IgG Secondary Antibody [NL493]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG


R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IgG Antibody (NL012)(6)

We have publications tested in 2 confirmed species: Human, Cercopithecus aethiops.

We have publications tested in 2 applications: Flow Cytometry, ICC.

Filter By Application
Flow Cytometry
All Applications
Filter By Species
Cercopithecus aethiops
All Species
Showing Publications 1 - 6 of 6.
Publications using NL012 Applications Species
Ryoo, H;Underhill, GH; Combinatorial Microgels for 3D ECM Screening and Heterogeneous Microenvironmental Culture of Primary Human Hepatic Stellate Cells bioRxiv : the preprint server for biology 2023-05-08 [PMID: 37214995] (ICC, Human) ICC Human
MS Szymczak, WK Reeves, MM Miller Further Characterization of Rio Grande Virus and Potential for Cross Reactivity with Rift Valley Fever Virus Assays Viruses, 2021-08-30;13(9):. 2021-08-30 [PMID: 34578299] (ICC, Cercopithecus aethiops) ICC Cercopithecus aethiops
T Kryza, C Parent, J Pardessus, A Petit, J Burlaud-Ga, P Reverdiau, S Iochmann, V Labas, Y Courty, N Heuzé-Vour Human kallikrein-related peptidase 12 stimulates endothelial cell migration by remodeling the fibronectin matrix Sci Rep, 2018-04-20;8(1):6331. 2018-04-20 [PMID: 29679011] (ICC, Human) ICC Human
TK Kim, CS Park, J Jang, MR Kim, HJ Na, K Lee, HJ Kim, K Heo, BC Yoo, YM Kim, JW Lee, SJ Kim, ES Kim, DY Kim, K Cha, TG Lee, S Lee Inhibition of VEGF-dependent angiogenesis and tumor angiogenesis by an optimized antibody targeting CLEC14a Mol Oncol, 2018-01-26;0(0):. 2018-01-26 [PMID: 29316206] (Flow Cytometry, Human) Flow Cytometry Human
Ki , M K, Jeoung , M H, Choi , J R, Rho , S-S, Kwon , Y-G, Shim , H, Chung , J, Hong , H J, Song , B D, Lee , S Human antibodies targeting the C-type lectin-like domain of the tumor endothelial cell marker clec14a regulate angiogenic properties in vitro. Oncogene, 2013-05-06;32(48):5449-57. 2013-05-06 [PMID: 23644659] (Flow Cytometry, Human) Flow Cytometry Human
Mikulak J, Teichberg S, Faust T, Schmidtmayerova H, Singhal PC HIV-1 harboring renal tubular epithelial cell interaction with T cells results in T cell trans-infection. Virology, 2008-12-17;385(1):105-14. 2008-12-17 [PMID: 19095275] (ICC, Human) ICC Human

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Understanding the Importance of Human IgG
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Gene Symbol IGHG1