Goat anti-Mouse IgG Secondary Antibody [Unconjugated]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Goat anti-Mouse IgG Secondary Antibody [Unconjugated] Summary

• Immunogen: Mouse IgG
• Specificity: Detects mouse IgG₁, IgG_2A, IgG_2B, IgG₃ heavy and light chains. In direct ELISAs, less than 15% cross-reactivity with human IgG and rabbit IgG is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IGHG1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:5000 dilution

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Goat anti-Mouse IgG Secondary Antibody [Unconjugated]

• IgG
• Immunoglobulin G
• ImmunoglobulinG

Background

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Antibody (AF007)(17)

Publications using AF007

• N Fakatava, H Mitarai, A Yuda, A Haraguchi, H Wada, D Hasegawa, H Maeda, N Wada Actin alpha 2, smooth muscle, a transforming growth factor-beta1-induced factor, regulates collagen production in human periodontal ligament cells via Smad2/3 pathway Journal of dental sciences, 2023;18(2):567-576. 2023 [PMID: 37021273] (Western Blot, Human)
• N Fakatava, H Mitarai, A Yuda, A Haraguchi, H Wada, D Hasegawa, H Maeda, N Wada Actin alpha 2, smooth muscle, a transforming growth factor-beta1-induced factor, regulates collagen production in human periodontal ligament cells via Smad2/3 pathway Journal of dental sciences, 2022-09-16;18(2):567-576. 2022-09-16 [PMID: 37021273] (Western Blot, Human)
• Mansky RH, Greguske EA, Yu D et al. Tumor suppressor p53 regulates heat shock factor 1 protein degradation in Huntington's disease Cell reports 3/2/2023 [PMID: 36867535]
• N Kurgan, B Baranowski, J Stoikos, AJ MacNeil, VA Fajardo, REK MacPherson, P Klentrou Characterization of sclerostin's response within white adipose tissue to an obesogenic diet at rest and in response to acute exercise in male mice Frontiers in Physiology, 2023-01-04;13(0):1061715. 2023-01-04 [PMID: 36685192] (Western Blot, Mouse, N/A)
• ShengPeng Wang, Bianbian Wang, Yue Shi, Tanja Möller, Rebekka I. Stegmeyer, Boris Strilic et al. Mechanosensation by endothelial PIEZO1 is required for leukocyte diapedesis Blood 7/21/2022 [PMID: 35443048]
• Mohammad Rahbar, Yuling Wu, J. Anand Subramony, Guozhen Liu Sensitive Colorimetric Detection of Interleukin-6 via Lateral Flow Assay Incorporated Silver Amplification Method Frontiers in Bioengineering and Biotechnology 11/26/2021 [PMID: 34900966]
• J Wang, Y Zhang, Y Xiao, X Yuan, P Li, X Wang, Y Duan, VL Seewaldt, D Yu Boosting immune surveillance by low-dose PI3K inhibitor facilitates early intervention of breast cancer American journal of cancer research, 2021-05-15;11(5):2005-2024. 2021-05-15 [PMID: 34094666] (Neutralization Control, Mouse)
• S Wu, M Luo, KKW To, J Zhang, C Su, H Zhang, S An, F Wang, D Chen, L Fu Intercellular transfer of exosomal wild type EGFR triggers osimertinib resistance in non-small cell lung cancer Molecular Cancer, 2021-01-18;20(1):17. 2021-01-18 [PMID: 33461557] (ICC, Western Blot, Human)
• RO Bello, MA Abdullah, R Abd Majid, VK Chin, MF Abd Rachma, ZO Ibraheem, MK Hussain, MG Magaji, R Basir IL35 modulation altered survival, cytokine environment and histopathological consequences during malaria infection in mice Malar. J., 2019-12-19;18(1):434. 12/19/2019 [PMID: 31856836]
• HA Himburg, M Roos, T Fang, Y Zhang, CM Termini, L Schlussel, MM Kim, A Pang, J Kan, L Zhao, H Suh, JP Sasine, G Sapparapu, PM Bowers, G Schiller, JP Chute Chronic myelogenous leukemia stem cells require cell-autonomous pleiotrophin signaling J. Clin. Invest., 2020-01-02;0(0):. 2020-01-02 [PMID: 31613796] (Bioassay, Human)
• Peijun J. W. He, Ioannis N. Katis, Robert W. Eason, Collin L. Sones Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique Biosensors (Basel) 10/20/2018 [PMID: 30347807]
• Chen D, Ireland S, Davis L, Kong X, Stowe A, Wang Y, White W, Herbst R, Monson N Autoreactive CD19+CD20- Plasma Cells Contribute to Disease Severity of Experimental Autoimmune Encephalomyelitis. J Immunol, 2016-01-13;196(4):1541-9. 2016-01-13 [PMID: 26764035] (ELISpot Development, Mouse)
• Bonapace L, Coissieux M, Wyckoff J, Mertz K, Varga Z, Junt T, Bentires-Alj M Cessation of CCL2 inhibition accelerates breast cancer metastasis by promoting angiogenesis. Nature, 2014-10-22;515(7525):130-3. 2014-10-22 [PMID: 25337873] (Bioassay, Mouse)
• Jaiswal M, Katara G, Mallers T, Chaouat G, Gilman-Sachs A, Beaman K Vacuolar-ATPase isoform a2 regulates macrophages and cytokine profile necessary for normal spermatogenesis in testis. J Leukoc Biol, 2014-03-12;96(2):337-47. 2014-03-12 [PMID: 24623332] (Neutralization, Mouse)
• Biancheri P, Di Sabatino A, Ammoscato F, Facciotti F, Caprioli F, Curciarello R, Hoque S, Ghanbari A, Joe-Njoku I, Giuffrida P, Rovedatti L, Geginat J, Corazza G, MacDonald T Absence of a role for interleukin-13 in inflammatory bowel disease. Eur J Immunol, 2014-02-01;44(2):370-85. 2014-02-01 [PMID: 24338958] (Western Blot, Human)
• Li N, Mao D, Lu S, Tong C, Zhang Y, Long M Distinct binding affinities of Mac-1 and LFA-1 in neutrophil activation. J Immunol, 2013-03-20;190(8):4371-81. 2013-03-20 [PMID: 23514737] (Neutralization, Human)
• Westenbrink BD, Ruifrok WP, Voors AA Vascular endothelial growth factor is crucial for erythropoietin-induced improvement of cardiac function in heart failure. Cardiovasc. Res., 2010-02-05;87(1):30-9. 2010-02-05 [PMID: 20139114] (Control, Rat)

Bioinformatics

• Gene Symbol: IGHG1