Recombinant Human IGFBP-5 Protein

Product Discontinued

Recombinant Human IGFBP-5 Protein Summary

• Description: A recombinant protein corresponding to 253 amino acids of Human IGFBP-5
• Preparation Method: >98% SDS-PAGE
• Details of Functionality: Biological Activity: The ED50 as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 is <0.3 mg/ml, in the presence of 15 ng/ml of human IGF-II.
  Results: <0.3 mg/ml
• Protein/Peptide Type: Recombinant Protein
• Gene: IGFBP5
• Purity: >98%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Theoretical MW: 28.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Sterile filtered and10 mM Sodium Citrate solution, pH 3.0.
• Concentration: Lyoph
• Purity: >98%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute with water to a final concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers or stored at 4C for 1 week or -20C for future use.

Notes

The purity of this protein is >95% pure by SDS-PAGE

Alternate Names for Recombinant Human IGFBP-5 Protein

• IBP5IBP-5
• IGF-binding protein 5
• IGFBP5
• IGFBP-5
• insulin-like growth factor binding protein 5
• insulin-like growth factor-binding protein 5

Background

Human IGF-BP5 is a 28.6 kDa cysteine-rich secreted protein produced by Vascular smooth muscle cells. It is the major IGF-binding protein present in bone tissue and helps potentiate the action of IGF-I on smooth muscle cells, fibroblasts or osteoblasts. Studies show that IGF-BP5 acts as a growth inhibitor and pro-apoptotic agent in breast cancer cells. IGF-BP5 overexpressing mice show an increase in neonatal mortality, reduced female fertility, whole body growth inhibition, and retarded muscle development. Recombinant human IGF-BP5 is a 28.6 kDa protein consisting of 253 amino acid residues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGFBP5