IGF-II/IGF2 Antibody (75015) [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: B/N
• Clone: 75015
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

IGF-II/IGF2 Antibody (75015) [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human IGF-II/IGF2
  Ala25-Glu91
  Accession # P01344.1
• Specificity: Detects human IGF-II/IGF2 in direct ELISA and Western blots. This antibody shows 100% cross-reactivity with recombinant mouse (rm) IGF‑II and no cross-reactivity with recombinant human IGF-I or rmIGF-I.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Neutralization

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGF-II/IGF2 Antibody (75015) [Alexa Fluor® 405]

• C11orf43
• chromosome 11 open reading frame 43
• FLJ22066
• FLJ44734
• GRDF
• IGF2
• IGF-2
• IGFII
• IGF-II
• insulin-like growth factor 2 (somatomedin A)
• insulin-like growth factor II
• insulin-like growth factor type 2
• MSA
• PEG2
• PP9974
• somatomedin-A

Background

Insulin-like growth factor I (also known as somatomedin C and somatomedin A) and insulin-like growth factor II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved (100% identity between human, bovine, and porcine proteins) and exhibit cross-species activity.

IGF-II is a potent mitogenic growth factor. However, unlike IGF-I which has important postnatal roles, the growth-promoting function of IGF-II is limited to embryonic development.

Two specific cell surface receptors that bind IGF-I and IGF-II have been identified. The type I IGF receptor that participates in IGF signaling is structurally related to the insulin receptor. It is a disulfide-linked heterotetrameric transmembrane glycoprotein with an intracellular tyrosine kinase domain. Type I IGF receptor binds IGF-I with higher affinity than IGF-II. The type II IGF receptor which binds IGF-II with much higher affinity than IGF-I is also the cation-independent mannose 6-phosphate receptor. At the present time, it is not known if the type II IGF receptor participates in the IGF signaling pathway. An additional unknown receptor which mediates IGF‑II signaling has also been proposed. Circulating IGFs exist in complexes bound to IGF binding proteins. Currently, at least six high affinity binding proteins have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.