Human IgA Isotype Control

SDS-Page: Human IgA Isotype Control [NBP1-97039] - SDS-PAGE of Human IgA Isotype control.Lane 1: Human IgA Isotype control, Reduced [5.0ug].Lane 2: Human IgA Isotype control, Reduced [1.0ug].Lane 3: Opal Prestained Molecular Weight Marker.Lane 4: Human IgA Isotype control, Non-Reduced [1.0ug].Lane 5: Human IgA Isotype control, Non-Reduced [5.0ug].4-20% Gel, Coomassie Blue Stained. Expected MW: ~160kDa Non-Reduced, ~55kDa, and 23kDa Reduced. Additional bands: monomer, dimer and trimer.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, PAGE
• Host: Human
• Conjugate: Unconjugated
• Format: Purified

Human IgA Isotype Control Summary

• Description: This product was prepared from human serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum and anti-Human IgA (a chain specific)
  
  Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
  
  No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.
• Specificity: No reaction was observed against anti-Human IgG F(c). Some light chain cross reactivity will occur with anti-Human IgG.
• Isotype: IgA
• Host: Human
• Gene: IGHA1
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry 1:10 - 1:500
  • SDS-Page
  • Western Blot 1:100 - 1:2000
• Application Notes: This product has been tested in SDS-Page and Western blot and can be utilized as a control or standard reagent in SDS, Western Blotting and ELISA experiments.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Alternate Names for Human IgA Isotype Control

• IgA
• Immunoglobulin A

Background

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. The most common feature of plasmacytomas, and certain non Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. This antibody is useful in the identification of leukemias, plasmacytomas, and certain non Hodgkin's lymphomas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgA Isotype Control (NBP1-97039)(1)

Publication using NBP1-97039

• Waterman HR, Dufort MJ, Posso SE et al. Lupus IgA1 autoantibodies synergize with IgG to enhance pDC responses to RNA-containing immune complexes bioRxiv 2023-09-12 [PMID: 37745328]

FAQs for IgA Isotype Control (NBP1-97039). (Showing 1 - 2 of 2 FAQ).

1. What is the source of the protein, is it native protein purified from serum or recombinant? !--EndFragment-->
   • This protein is native human IgA protein that was purified out from human serum by delipidation, selective precipitation, and tandem molecular sieve chromatography.
2. Can you provide more details on your product "Human IgA Full length Protein"?
   •  Immunoglobulin A is the primary responder in muscosal immunity, and comprises close to 75% of the total immunoglobulin produced. IgA can also be secreted and is protected from degradation by many proteolytic enzymes (which allows it to be secreted along the gastrointestinal tract). Immunoglobulin A only weakly activates the complement system and is not readily opsonized. Human IgA whole molecule was prepared from human serum by a multi-step process which includes delipidation, selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Human IgA whole molecule was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum and anti-Human IgA (a chain specific). No reaction was observed against anti-Human IgG F(c). Some light chain cross reactivity will occur with anti-Human IgG.

Bioinformatics

• Gene Symbol: IGHA1