Mouse IgA ELISA Kit (Colorimetric) Summary
| Description |
IgA (Mouse) ELISA Kit is a highly sensitive two-site enzyme-linked immunoassay for the quantitative determination of IgA in mouse biological samples. |
| Standard Curve Range |
12.5 to 400 ng/mL |
| Sensitivity |
1.167 |
| Assay Type |
Two-site enzyme-linked immunoassay |
| Sample Volume |
100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
IGHA1 |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Useful for quantification. |
Packaging, Storage & Formulations
Kit Components
|
Components
|
- Anti-Mouse IgA ELISA Micro Plate: Twelve removable eight (8) well micro well strips in wellholder frame. Each well is coated with affinity purified anti-Mouse IgA
- Chromogen-Substrate Solution: One vial containing 3,3’,5,5’-tetramethybenzidine (TMB)and hydrogen peroxide in citric acid buffer at pH 3.3.
- Diluent Concentrate (Running Buffer): One bottle containing 5X concentrated diluentrunning buffer.
- Enzyme-Antibody Conjugate 100X: One vial containing affinity purified anti-Mouse IgAantibody conjugated with horseradish peroxidase in a stabilizing buffer.
- Mouse IgA Calibrator: One vial containing a lyophilized Mouse IgA calibrator.
- Stop Solution: One vial containing 0.3 M sulfuric acid. WARNING: Avoid contact with skin
- Wash Solution Concentrate: One bottle containing a 20X concentrated wash solution
|
Notes
This product is produced by and distributed for Abnova, a company based in Taiwan.
Alternate Names for Mouse IgA ELISA Kit (Colorimetric)
Background
Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. The most common feature of plasmacytomas, and certain non Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. This antibody is useful in the identification of leukemias, plasmacytomas, and certain non Hodgkin's lymphomas.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for IgA ELISA Kit (KA1943). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.