IFT74 Antibody

Western Blot: IFT74 Antibody [NB300-822] - Staining of HEK293 cell lysate (35 ug protein in RIPA buffer). Antinbody at 0.2 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

IFT74 Antibody Summary

• Description: Novus Biologicals Goat IFT74 Antibody (NB300-822) is a polyclonal antibody validated for use in WB and ELISA. Anti-IFT74 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KTIVDALHSTSGN corresponding to C-Terminus according to NP_001092692.1, NP_001092693.1, NP_079379.2, AAK77221.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IFT74
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.2 - 0.6 ug/mL
• Application Notes: IF successful usage for stained rat cortical neurons is reported in scientific literature (PMID: 17166276). WB: Approx. 70-75 kDa band observed in HEK293 lysates is reported in scientific literature (PMID: 17166276).
• Control:

  293 Whole Cell Lysate

Reactivity Notes

Expected species reactivity based on sequence similarity: Rat

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IFT74 Antibody

• Capillary morphogenesis gene 1 protein
• CCDC2capillary morphogenesis protein 1
• CMG1Coiled-coil domain-containing protein 2
• CMG-1MGC111562
• coiled-coil domain containing 2
• FLJ22621
• intraflagellar transport 74 homolog (Chlamydomonas)
• intraflagellar transport protein 74 homolog

Background

The IFT74 gene codes for an intraflagellar transport protein 74 homolog. Isoform 1 is 600 amino acids long at 69 kDA while isoform 2 exists at 409 amino acids long at 46 kDA. IFT74 has been linked to dementia, lateral sclerosis, neuronitis, amyotrphic lateral sclerosis, and frontotemporal dementia. It is known to interact with other genes such as: IFT27, IFT52, IFT81, IFT88, and DYNC2LI1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFT74 Antibody (NB300-822)(3)

Publications using NB300-822

• kanie T, Abbott kL, MooneY NA et al. The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base. Dev. Cell. 2017-07-10 [PMID: 28625565]
• Momeni P, Schymick J, Jain S et al. Analysis of IFT74 as a candidate gene for chromosome 9p-linked ALS-FTD. BMC Neurol 2006-12-01 [PMID: 17166276]
• Bell SE, Mavila A, Salazar R et al. Differential gene expression during capillary morphogenesis in 3D collagen matrices: regulated expression of genes involved in basement membrane matrix assembly, cell cycle progression, cellular differentiation and G-protein signaling. J Cell Sci 2001-08-01 [PMID: 11683410]

Bioinformatics

• Gene Symbol: IFT74
• Entrez:
  • 80173(Human)
  • 313365(Rat)
• Uniprot:
  • AAK77221.1(Human)
  • NP_001092692.1(Human)
  • NP_001092693.1(Human)
  • NP_079379.2(Human)