IFT74 Antibody

Images

 
Western Blot: IFT74 Antibody [NB300-822] - (0.3ug/ml) of 293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: IFT74 Antibody [NB300-822] - A) (2.5mg/ml) staining of primary rat cortical neurons showed localization of IFT74 to vesicles in the cell body and along the neuronal processes. B) ...read more
Immunohistochemistry-Paraffin: IFT74 Antibody [NB300-822] - IHC analysis of IFT74 in paraffin embedded Human Liver using NB300-822 at 5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

IFT74 Antibody Summary

Immunogen
Peptide with sequence C-KTIVDALHSTSGN corresponding to C-Terminus according to NP_001092692.1, NP_001092693.1, NP_079379.2, AAK77221.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IFT74
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 5 - 10 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
IF successful usage for stained rat cortical neurons is reported in scientific literature (PMID: 17166276). WB: Approx. 70-75 kDa band observed in HEK293 lysates is reported in scientific literature (PMID: 17166276). IHC-P: Human liver shows vesicular staining in cytoplasm of hepatocytes.
Positive Control
293 Lysate (NB800-PC6)
Publications
Read Publications using NB300-822.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IFT74 Antibody

  • Capillary morphogenesis gene 1 protein
  • CCDC2capillary morphogenesis protein 1
  • CMG1Coiled-coil domain-containing protein 2
  • CMG-1MGC111562
  • coiled-coil domain containing 2
  • FLJ22621
  • intraflagellar transport 74 homolog (Chlamydomonas)
  • intraflagellar transport protein 74 homolog

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IFT74 Antibody (NB300-822)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IFT74
Entrez
Uniprot