IFN-beta Antibody (944233) [Unconjugated]

Recombinant Mouse IFN‑beta protein was serially diluted 2-fold and captured by Rat Anti-Mouse IFN‑beta Monoclonal Antibody (Catalog # MAB82342) coated on a Clear Polystyrene Microplate (DY990). Rat Anti-Mouse IFN‑beta Monoclonal Antibody (MAB82341) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

• Reactivity: Mu
• Applications: ELISA
• Clone: 944233
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

IFN-beta Antibody (944233) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell HEK293-derived Mouse IFN‑ beta
  Ile22-Asn182
  Accession # P01575
• Specificity: Detects mouse IFN‑ beta in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IFN-beta Antibody (944233) [Unconjugated]

• Fibroblast interferon
• IFB
• IFBIFNB
• IFF
• IFNB
• IFNB1
• IFNbeta
• IFN-beta
• interferon beta
• interferon, beta 1, fibroblast
• MGC96956

Background

Interferon beta (IFN-beta ), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules (1). Mature mouse IFN-beta shares 75% and 47% amino acid sequence identity with the rat and human proteins, respectively. Fibroblasts are the major producers of IFN-beta , but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogens (2). It is transcriptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B (3, 4). IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) (5). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS (6-10). IFN-beta can additionally induce the expression of the anti-inflammatory cytokine IL-10 (11).

1. González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.
2. Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.
3. Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.
4. Häcker, H. et al. (2006) Nature 439:204.
5. Teige, I. et al. (2003) J. Immunol. 170:4776.
6. Shinohara, M.L. et al. (2008) Immunity 29:68.
7. Guo, B. et al. (2008) J. Clin. Invest. 118:1680.
8. Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab. Immune Disord. Drug Targets 10:161.
9. Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.
10. Inoue, M. and M.L. Shinohara (2013) Immunology 139:11.
11. Wang, H. et al. (2011) J. Immunol 186:675.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P01575()