IDH3A Antibody

Western Blot: IDH3A Antibody [H00003419-B01P] - Analysis of IDH3A expression in rat brain.

Immunocytochemistry/Immunofluorescence: IDH3A Antibody [H00003419-B01P] - Analysis of purified antibody to IDH3A on HeLa cell. (antibody concentration 10 ug/ml)

Western Blot: IDH3A Antibody [H00003419-B01P] - Analysis of IDH3A expression in A-431.

Western Blot: IDH3A Antibody [H00003419-B01P] - Analysis of IDH3A expression in transfected 293T cell line by IDH3A polyclonal antibody. Lane 1: IDH3A transfected lysate(40.26 KDa). Lane 2: Non-transfected lysate.

Product Discontinued

IDH3A Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: IDH3A (NP_005521.1, 1 a.a. - 366 a.a.) full-length human protein. MAGPAWISKVSRLLGAFHNPKQVTRGFTGGVQTVTLIPGDGIGPEISAAVMKIFDAAKAPIQWEERNVTAIQGPGGKWMIPSEAKESMDKNKMGLKGPLKTPIAAGHPSMNLLLRKTFDLYANVRPCVSIEGYKTPYTDVNIVTIRENTEGEYSGIEHVIVDGVVQSIKLITEGASKRIAEFAFEYARNNHRSNVTAVHKANIMRMSDGLFLQKCREVAESCKDIKFNEMYLDTVCLNMVQDPSQFDVLVMPNLYGDILSDLCAGLIGGLGVTPSGNIGANGVAIFESVHGTAPDIAGKDMANPTALLLSAVMMLRHMGLFDHAARIEAACFATIKDGKSLTKDLGGNAKCSDFTEEICRRVKDLD
• Specificity: IDH3A - isocitrate dehydrogenase 3 (NAD+) alpha,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: IDH3A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control. It is also useful for IF.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for IDH3A Antibody

• EC 1.1.1
• EC 1.1.1.41
• H-IDH alpha
• isocitrate dehydrogenase (NAD+) alpha chain
• isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
• isocitrate dehydrogenase 3 (NAD+) alpha
• Isocitric dehydrogenase subunit alpha
• NAD(+)-specific ICDH subunit alpha
• NAD(H)-specific isocitrate dehydrogenase alpha subunit
• NAD+-specific ICDH

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for IDH3A Antibody (H00003419-B01P) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Hongxia Hu WB Human 08/05/2010

Summary

• Application: Western Blot
• Sample Tested: HCT116 whole cell lysate, Sample Amount: 70ug
• Species: Human

Bioinformatics

• Gene Symbol: IDH3A
• Entrez:
  • 3419(Human)
• Uniprot:
  • NP_005521.1(Human)