ID4 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: ID4 Antibody [NBP2-56322] - Staining of human cell line HEK 293 shows localization to nucleus. Antibody staining is shown in green.

ChIP-Exo-Seq composite graph for Anti-ID4 (NBP2-56322) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• Reactivity: Hu, Rt, Mu
• Applications: ICC/IF, ChIP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ID4 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: PLTALNTDPAGAVNKQGDSILCR
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ID4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation-exo-Seq 1-10ug per reaction
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
• Application Notes: ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  ID4 Recombinant Protein Antigen (NBP2-56322PEP)

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:31828317).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for ID4 Antibody - BSA Free

• bHLHb27BHLHB27
• Class B basic helix-loop-helix protein 27
• DNA-binding protein inhibitor ID-4
• IDB4
• Inhibitor of DNA binding 4
• inhibitor of DNA binding 4, dominant negative helix-loop-helix protein

Background

Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes ina number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo-and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain theHLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA andtranscriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995 (PubMed7665172)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ID4 Antibody (NBP2-56322)(1)

Publication using NBP2-56322

• Aprato J, Sock E, Weider M, et al. miR-130b is a Potent Stimulator of Hepatic VLDL Assembly and Secretion via Marked Induction of Microsomal Triglyceride Transfer Protein (MTP) Nucleic Acids Res. 2019-12-10 [PMID: 31828317] (ICC/IF, Rat)

Bioinformatics

• Gene Symbol: ID4