ID2 Antibody (A4-D4)

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clone: A4-D4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 2 mg/ml

ID2 Antibody (A4-D4) Summary

• Immunogen: Recombinant protein with Human ID2 aa 1- 80
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ID2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100
  • Western Blot 1:1000-1:10000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 40% Glycerol, 0.2% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein A purified

Alternate Names for ID2 Antibody (A4-D4)

• BHLHB26
• bHLHb26cell growth-inhibiting gene 8
• Class B basic helix-loop-helix protein 26
• DNA-binding protein inhibitor ID2
• DNA-binding protein inhibitor ID-2
• GIG8
• helix-loop-helix protein ID2
• ID2
• ID2A
• ID2H
• inhibitor of differentiation 2
• Inhibitor of DNA binding 2
• inhibitor of DNA binding 2, dominant negative helix-loop-helix protein
• MGC26389

Background

ID2 belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the ID family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. Recombinant human ID2 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ID2