ICT Antibody

Immunoprecipitation: ICT Antibody [NBP2-76358] - Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ICT1 antibody NBP2-76358 used for IP at 15 ul per reaction.

Product Discontinued

ICT Antibody Summary

• Immunogen: Immunogen sequence between amino acids 156 and 206
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MRPL58
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 15-20 ul/mg lysate

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Tris-buffered Saline, 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.4 mg/ml
• Purity: Antigen Affinity-purified

Alternate Names for ICT Antibody

• Digestion substraction 1
• DS-1DS1
• EC 3.1.1.29
• Immature colon carcinoma transcript 1 protein
• immature colon carcinoma transcript 1
• peptidyl-tRNA hydrolase ICT1, mitochondrial

Background

ICT1 is an essential peptidyl-tRNA hydrolase component of the mitochondrial large ribosomal subunit. Acts as a codon-independent translation release factor that has lost all stop codon specificity and directs the termination of translation in mitochondrion, possibly in case of abortive elongation. May be involved in the hydrolysis of peptidyl-tRNAs that have been prematurely terminated and thus in the recycling of stalled mitochondrial ribosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MRPL58