Human ICAM-2/CD102 ELISA Kit (Colorimetric)

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Human ICAM-2/CD102 ELISA Kit [NBP1-91181] - Data for Human sICAM-2 Platinum ELISA

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), amniotic fluid, urine, whole blood
Standard Curve Range
0.75 - 24.0 U/mL
Sensitivity
0.30 U/mL

Order Details

Human ICAM-2/CD102 ELISA Kit (Colorimetric) Summary

Specificity
This antibody targets the soluble protein. The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into ICAM-2 positive serum. There was no crossreactivity detected, notably not with ICAM-1 and ICAM-3.
Standard Curve Range
0.75 - 24.0 U/mL
Sensitivity
0.30 U/mL
Assay Type
Sandwich ELISA
Inter-Assay
7.00%
Intra-Assay
6.70%
Sample Volume
100 uL (1:20 prediluted)
Kit Type
ELISA Kit (Colorimetric)
Gene
ICAM2

Applications/Dilutions

Application Notes
This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotin Conjugate Diluent
  2. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sICAM-2
  3. Adhesive Films
  4. Biotin-Conjugate anti-human sICAM-2 monoclonal antibody
  5. Human sICAM-2 Standard lyophilized, 24.00 U/ml upon reconstitution
  6. Stop Solution (1.8N H2SO4)
  7. Standard Buffer Diluent (10x)
  8. Wash Buffer Concentrate 200x
  9. Streptavidin-HRP Diluent
  10. Streptavidin-HRP-Concentrate
  11. Substrate Solution (tetramethyl-benzidine and buffered hydrogen peroxide)

Alternate Names for Human ICAM-2/CD102 ELISA Kit (Colorimetric)

  • CD102 antigen
  • CD102
  • ICAM2
  • ICAM-2
  • intercellular adhesion molecule 2

Background

Intercellular Adhesion Molecule-2 (ICAM-2) is a 60 kDa surface glycoprotein that promotes adhesion between immune cells and the vascular endothelium. ICAM-2 is a member of the Ig-superfamily with two immunoglobulin-like extracellular domains. ICAM-2 is much more closely related to the two N-terminal domains of ICAM-1 (34% identity) than either ICAM-1 or ICAM-2 is to other members of the Ig-superfamily, demonstrating the existence of a subfamily of Ig-like ligands that bind the same integrin receptor. ICAM-2 is broadly distributed on hematopoietic cells. On resting lymphocytes, the ICAM-2 expression is several-fold higher than that seen with ICAM-1, while monocytes express equivalent levels of ICAM-1 and ICAM-2. In contrast to ICAM-1, neutrophils stain negative for ICAM-2. Just little or no ICAM-2 staining can be demonstrated on other cell lines, the sole exception being resting vascular endothelial cells, which possess high levels of ICAM-2, and small clusters of cells in lymphoid tissue germinal centres. ICAM-2 expression is unaffected by a variety of inflammatory cytokines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol ICAM2
Entrez