Human ICAM-2/CD102 ELISA Kit (Colorimetric) Summary
This antibody targets the soluble protein. The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into ICAM-2 positive serum. There was no crossreactivity detected, notably not with ICAM-1 and ICAM-3.
|Standard Curve Range
0.75 - 24.0 U/mL
100 uL (1:20 prediluted)
ELISA Kit (Colorimetric)
This kit is an enzyme-linked immunosorbent assay for quantitative detection.
Packaging, Storage & Formulations
Storage of components varies. See protocol for specific instructions.
- Biotin-Conjugate anti-human sICAM-2 monoclonal antibody
- Biotin Conjugate Diluent
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sICAM-2
- Adhesive Films
- Stop Solution (1.8N H2SO4)
- Streptavidin-HRP Diluent
- Standard Buffer Diluent (10x)
- Human sICAM-2 Standard lyophilized, 24.00 U/ml upon reconstitution
- Substrate Solution (tetramethyl-benzidine and buffered hydrogen peroxide)
- Wash Buffer Concentrate 200x
Alternate Names for Human ICAM-2/CD102 ELISA Kit (Colorimetric)
- CD102 antigen
- intercellular adhesion molecule 2
Intercellular Adhesion Molecule-2 (ICAM-2) is a 60 kDa surface glycoprotein that promotes adhesion between immune cells and the vascular endothelium. ICAM-2 is a member of the Ig-superfamily with two immunoglobulin-like extracellular domains. ICAM-2 is much more closely related to the two N-terminal domains of ICAM-1 (34% identity) than either ICAM-1 or ICAM-2 is to other members of the Ig-superfamily, demonstrating the existence of a subfamily of Ig-like ligands that bind the same integrin receptor. ICAM-2 is broadly distributed on hematopoietic cells. On resting lymphocytes, the ICAM-2 expression is several-fold higher than that seen with ICAM-1, while monocytes express equivalent levels of ICAM-1 and ICAM-2. In contrast to ICAM-1, neutrophils stain negative for ICAM-2. Just little or no ICAM-2 staining can be demonstrated on other cell lines, the sole exception being resting vascular endothelial cells, which possess high levels of ICAM-2, and small clusters of cells in lymphoid tissue germinal centres. ICAM-2 expression is unaffected by a variety of inflammatory cytokines.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed
for 6 months from date of receipt.
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