Human ICAM-2/CD102 ELISA Kit (Colorimetric)

ELISA: Human ICAM-2/CD102 ELISA Kit (Colorimetric) [NBP1-91181] - man ICAM-2/CD102 ELISA Kit [NBP1-91181] - Data for Human sICAM-2 Platinum ELISA

Product Discontinued

Human ICAM-2/CD102 ELISA Kit (Colorimetric) Summary

• Specificity: This antibody targets the soluble protein. The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into ICAM-2 positive serum. There was no crossreactivity detected, notably not with ICAM-1 and ICAM-3.
• Standard Curve Range: 0.75 - 24.0 U/mL
• Sensitivity: 0.30 U/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 7.00%
• Intra-Assay: CV% < 6.70%
• Sample Volume: 100 uL (1:20 prediluted)
• Kit Type: ELISA Kit (Colorimetric)
• Gene: ICAM2

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human ICAM-2/CD102 ELISA Kit (Colorimetric)

• CD102 antigen
• CD102
• ICAM2
• ICAM-2
• intercellular adhesion molecule 2

Background

Intercellular Adhesion Molecule-2 (ICAM-2) is a 60 kDa surface glycoprotein that promotes adhesion between immune cells and the vascular endothelium. ICAM-2 is a member of the Ig-superfamily with two immunoglobulin-like extracellular domains. ICAM-2 is much more closely related to the two N-terminal domains of ICAM-1 (34% identity) than either ICAM-1 or ICAM-2 is to other members of the Ig-superfamily, demonstrating the existence of a subfamily of Ig-like ligands that bind the same integrin receptor. ICAM-2 is broadly distributed on hematopoietic cells. On resting lymphocytes, the ICAM-2 expression is several-fold higher than that seen with ICAM-1, while monocytes express equivalent levels of ICAM-1 and ICAM-2. In contrast to ICAM-1, neutrophils stain negative for ICAM-2. Just little or no ICAM-2 staining can be demonstrated on other cell lines, the sole exception being resting vascular endothelial cells, which possess high levels of ICAM-2, and small clusters of cells in lymphoid tissue germinal centres. ICAM-2 expression is unaffected by a variety of inflammatory cytokines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for ICAM-2/CD102 ELISA Kit (NBP1-91181). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ICAM2