ICAM-2/CD102 Antibody (CBRIC2/2) - BSA Free

Flow Cytometry: ICAM-2/CD102 Antibody (CBRIC2/2) [NBP2-00320] - Analysis using the Biotin conjugate of NBP2-00320. Staining of peripheral blood monocytes with MOUSE ANTI HUMAN CD102: Biotin.

Product Discontinued

ICAM-2/CD102 Antibody (CBRIC2/2) - BSA Free Summary

• Additional Information: Clone CBRIC2/2 was used by HLDA to establish CD designation.
• Immunogen: The immunogen for this antibody is ICAM2.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ICAM2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: This CBRIC2/2 antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.125 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The CBRIC2/2 antibody has also been found useful for immunoblotting of a human peripheral blood cell lysate, recognizing a protein of approximately 55 kDa under non-reducing conditions. This CBRIC2/2 antibody has also been reported for use in immunoprecipitation, immunohistochemical staining and functional assays. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for ICAM-2/CD102 Antibody (CBRIC2/2) - BSA Free

• CD102 antigen
• CD102
• ICAM2
• ICAM-2
• intercellular adhesion molecule 2

Background

The CBRIC2/2 monoclonal antibody reacts with human ICAM-2 (CD102). ICAM-2 is a 55-65 kD transmembrane glycoprotein possessing 2 extracellular Ig domains, a single transmembrane domain, and a short 26-amino acid cytoplasmic domain. ICAM-2 is expressed on most leukocytes, and is strongly expressed on vascular endothelial cells. ICAM-2 is a ligand of integrin CD11a/CD18 (LFA-1), and also has been reported to bind integrin CD11b/CD18 (Mac-1). ICAM-2 is an intercellular adhesion molecule involved in lymphocyte homing to sites of inflammation. The CBRIC2/2 antibody has been reported to have blocking activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ICAM-2/CD102 Antibody (NBP2-00320)(1)

Publication using NBP2-00320

• Vazquez Rodriguez G, Abrahamsson A, Jensen LD, Dabrosin C. Estradiol Promotes Breast Cancer Cell Migration via Recruitment and Activation of Neutrophils. Cancer Immunol Res. 2017-02-03 [PMID: 28159748]

Bioinformatics

• Gene Symbol: ICAM2