ICAM-2/CD102 Antibody (CBRIC2/2)

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Flow Cytometry: ICAM-2/CD102 Antibody (CBRIC2/2) [NBP2-00320] - Analysis using the Biotin conjugate of NBP2-00320. Staining of peripheral blood monocytes with MOUSE ANTI HUMAN CD102: Biotin.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, Func, IHC, IHC-P, IP
Clone
CBRIC2/2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

ICAM-2/CD102 Antibody (CBRIC2/2) Summary

Immunogen
The immunogen for this antibody is ICAM2.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ICAM2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Functional
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
This CBRIC2/2 antibody has been tested by flow cytometric analysis of human peripheral blood cells. This can be used at less than or equal to 0.125 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The CBRIC2/2 antibody has also been found useful for immunoblotting of a human peripheral blood cell lysate, recognizing a protein of approximately 55 kDa under non-reducing conditions. This CBRIC2/2 antibody has also been reported for use in immunoprecipitation, immunohistochemical staining and functional assays. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Publications
Read Publication using NBP2-00320.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for ICAM-2/CD102 Antibody (CBRIC2/2)

  • CD102 antigen
  • CD102
  • ICAM2
  • ICAM-2
  • intercellular adhesion molecule 2

Background

The CBRIC2/2 monoclonal antibody reacts with human ICAM-2 (CD102). ICAM-2 is a 55-65 kD transmembrane glycoprotein possessing 2 extracellular Ig domains, a single transmembrane domain, and a short 26-amino acid cytoplasmic domain. ICAM-2 is expressed on most leukocytes, and is strongly expressed on vascular endothelial cells. ICAM-2 is a ligand of integrin CD11a/CD18 (LFA-1), and also has been reported to bind integrin CD11b/CD18 (Mac-1). ICAM-2 is an intercellular adhesion molecule involved in lymphocyte homing to sites of inflammation. The CBRIC2/2 antibody has been reported to have blocking activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ICAM-2/CD102 Antibody (NBP2-00320)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP2-00320AF405
Alexa Fluor 488 Labeled NBP2-00320AF488
Alexa Fluor 647 Labeled NBP2-00320AF647
Alexa Fluor 700 Labeled NBP2-00320AF700
Allophycocyanin Labeled NBP2-00320APC
DyLight 350 Labeled NBP2-00320UV
DyLight 405 Labeled NBP2-00320V
DyLight 488 Labeled NBP2-00320G
DyLight 680 Labeled NBP2-00320FR
DyLight 755 Labeled NBP2-00320IR
PE Labeled NBP2-00320PE
PerCP Labeled NBP2-00320PCP

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Bioinformatics Tool for ICAM-2/CD102 Antibody (NBP2-00320)

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Bioinformatics

Gene Symbol ICAM2
Entrez