ICAM-2/CD102 Antibody (B-T1) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IP
• Clone: B-T1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

ICAM-2/CD102 Antibody (B-T1) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Additional Information: Clone B-T1 was used by HLDA to establish CD designation.
• Immunogen: ICAM-2 transfected CHO cells
• Specificity: Recognizes the human CD102 cell surface molecule, a 55-65kD glycoprotein also known as ICAM-2. NB100-66624 inhibits cell adhesion and T cell activation and also recognizes soluble ICAM-2.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ICAM2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for ICAM-2/CD102 Antibody (B-T1) [CoraFluor™ 1]

• CD102 antigen
• CD102
• ICAM2
• ICAM-2
• intercellular adhesion molecule 2

Background

The 3C4 (mIC2/4) monoclonal antibody reacts with mouse ICAM-2 (CD102) cell surface glycoprotein, a ligand for the leucocyte integrin CD11a/CD18 (LFA-1).1 CD102 is constitutively expressed on T cells, B cells, and at high levels on vascular endothelial cells. (2) It is also expressed on a variety of leukocyte cell lines. (1) The 3C4 antibody blocks the interaction between ICAM-2 and LFA-1. (1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ICAM2