IBSP/Sialoprotein II Overexpression Lysate

Images

 
Western Blot: Sialoprotein II Overexpression Lysate (Adult Normal) [NBP2-10516] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Sialoprotein II.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

IBSP/Sialoprotein II Overexpression Lysate Summary

Description

IBSP/Sialoprotein II Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC222901

Accession#: NM_004967

Protein Tag: C-MYC/DDK
Gene
IBSP

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
33.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for IBSP/Sialoprotein II Overexpression Lysate

  • BNSP
  • Bone sialoprotein 2
  • Bone sialoprotein
  • BSP 2
  • BSP II
  • BSP
  • BSP2
  • BSPII
  • BSP-II
  • Cell binding sialoprotein
  • IBSP
  • Integrin binding sialoprotein
  • SP II
  • SPII
  • SP-II

Background

The protein encoded by the Sialoprotein II gene is a major structural protein of the bone matrix. It constitutes approximately 12% ofthe noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophicchondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is thetrophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cellattachment through an RGD sequence that recognizes the vitronectin receptor. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol IBSP