Mouse IBSP/Sialoprotein II ELISA Kit (Colorimetric)

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Mouse IBSP/Sialoprotein II ELISA Kit (Colorimetric) - Standard Curve Reference

Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids.
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL

Order Details

Mouse IBSP/Sialoprotein II ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Mouse BSP in samples. No significant cross-reactivity or interference between Mouse BSP and analogues was observed.
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.3%
Intra-Assay
CV% < 4.38%
Spike Recovery
86-110%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
IBSP

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Certificate of Analysis
  3. Concentrated Biotinylated Detection Ab (100×)
  4. Concentrated HRP Conjugate (100×)
  5. Concentrated Wash Buffer (25×)
  6. HRP Conjugate Diluent
  7. Micro ELISA Plate(Dismountable)
  8. Plate Sealer
  9. Product Description
  10. Reference Standard & Sample Diluent
  11. Reference Standard
  12. Stop Solution
  13. Substrate Reagent

Alternate Names for Mouse IBSP/Sialoprotein II ELISA Kit (Colorimetric)

  • BNSP
  • Bone sialoprotein 2
  • Bone sialoprotein
  • BSP 2
  • BSP II
  • BSP
  • BSP2
  • BSPII
  • BSP-II
  • Cell binding sialoprotein
  • IBSP
  • Integrin binding sialoprotein
  • SP II
  • SPII
  • SP-II

Background

The protein encoded by the Sialoprotein II gene is a major structural protein of the bone matrix. It constitutes approximately 12% ofthe noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophicchondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is thetrophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cellattachment through an RGD sequence that recognizes the vitronectin receptor. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol IBSP