IBSP/Sialoprotein II Antibody

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IBSP/Sialoprotein II Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human IBSP/Sialoprotein II
  Phe17-Gln317
  Accession # AAC95490
• Specificity: Detects human IBSP/Sialoprotein II in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IBSP
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IBSP/Sialoprotein II Antibody

• BNSP
• Bone sialoprotein 2
• Bone sialoprotein
• BSP 2
• BSP II
• BSP
• BSP2
• BSPII
• BSP-II
• Cell binding sialoprotein
• IBSP
• Integrin binding sialoprotein
• SP II
• SPII
• SP-II

Background

IBSP (integrin-binding sialoprotein; also BSP or bone sialoprotein (II)) is a 55‑75 kDa, secreted, variably glycosylated, monomeric noncollagenous member of the SIBLING family of extracellular matrix (ECM) proteins (1‑3). It is principally associated with the early stages of bone mineralization. BSP is synthesized as a 317 amino acid (aa) precursor that contains a 16 aa signal sequence and a 301 aa mature region (4‑6). The mature segment is divided into a basic N-terminus (aa 17‑62), a central region (aa 63‑233), and an acidic C‑terminus (aa 234‑317) (7).

Functional segments associated with the mature molecule include a type I collagen binding domain (aa 19‑46), two non‑RGD cell binding sites (aa 30‑57 and 261‑281), an RGD alpha _v beta ₃ integrin‑binding site (aa 286‑288) and two potential hydroxyapatite (HAp) nucleation domains (aa 76‑83 and 151‑158) (3, 4, 8‑11). HAp formation requires a BSP nucleation site composed of at least eight consecutive glutamic acid residues and, likely, a contribution from a BSP‑associated co‑nucleator (10, 12). BSP is highly glycosylated, sulfated, and phosphorylated. Phosphorylation may impact HAp growth, while carbohydrate may regulate cell adhesion (1, 3, 13). Mature human BSP is 70%, 72%, 78%, and 72% aa identical to porcine, rat, canine, and mouse BSP, respectively. BSP is synthesized by megakaryocytes/platelets, osteoblasts, osteocytes, odontoblasts, osteoclasts, and bone marrow stromal cells (14‑17).

1. Qin, C. et al. (2004) Crit. Rev. Oral Biol. Med. 15:126.
2. Alford, A.I. and K.D. Hankenson (2006) Bone 38:749.
3. Ganss, B. et al. (1999) Crit. Rev. Oral Biol. Med. 10:79.
4. Fisher, L.W. et al. (1990) J. Biol. Chem. 265:2347.
5. Kerr, J.M. et al. (1993) Genomics 17:408.
6. Kim, R.H. et al. (1994) Matrix Biol. 14:31.
7. Zaia, J. et al. (2001) Biochemistry 40:12983.
8. Tye, C.E. et al. (2005) J. Biol. Chem. 280:13487.
9. Stubbs, J.T. et al. (1997) J. Bone Miner. Res. 12:1210.
10. Tye, C.E. et al. (2003) J. Biol. Chem. 278:7949.
11. Miyauchi, A. et al. (1991) J. Biol. Chem. 266:20369.
12. Hakki, S.S. et al. (2006) J. Periodontol. 77:167.
13. Wazen, R.M. et al. (2007) J. Histochem. Cytochem. 55:35.
14. Kacena, M.A. et al. (2006) Bone 39:978.
15. Bianco, P. et al. (1991) Calcif. Tissue Int. 49:421.
16. Chen, J. et al. (1992) J. Bone Miner. Res. 7:987.
17. Kreke, M.R. et al. (2005) Bone 36:1047.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IBSP/Sialoprotein II Antibody (AF4014)(1)

Publication using AF4014

• K Wu, J Feng, F Lyu, F Xing, S Sharma, Y Liu, SY Wu, D Zhao, A Tyagi, RP Deshpande, X Pei, MG Ruiz, H Takahashi, S Tsuzuki, T Kimura, YY Mo, Y Shiozawa, R Singh, K Watabe Exosomal miR-19a and IBSP cooperate to induce osteolytic bone metastasis of estrogen receptor-positive breast cancer Nature Communications, 2021;12(1):5196. 2021 [PMID: 34465793] (Binding Assay, Human)

Bioinformatics

• Gene Symbol: IBSP
• Uniprot:
  • AAC95490()