IBSP/Sialoprotein II Antibody

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

IBSP/Sialoprotein II Antibody Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human IBSP/Sialoprotein II
Phe17-Gln317
Accession # AAC95490
Specificity
Detects human IBSP/Sialoprotein II in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IBSP
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
Publications
Read Publication using
AF4014 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IBSP/Sialoprotein II Antibody

  • BNSP
  • Bone sialoprotein 2
  • Bone sialoprotein
  • BSP 2
  • BSP II
  • BSP
  • BSP2
  • BSPII
  • BSP-II
  • Cell binding sialoprotein
  • IBSP
  • Integrin binding sialoprotein
  • SP II
  • SPII
  • SP-II

Background

IBSP (integrin-binding sialoprotein; also BSP or bone sialoprotein (II)) is a 55‑75 kDa, secreted, variably glycosylated, monomeric noncollagenous member of the SIBLING family of extracellular matrix (ECM) proteins (1‑3). It is principally associated with the early stages of bone mineralization. BSP is synthesized as a 317 amino acid (aa) precursor that contains a 16 aa signal sequence and a 301 aa mature region (4‑6). The mature segment is divided into a basic N-terminus (aa 17‑62), a central region (aa 63‑233), and an acidic C‑terminus (aa 234‑317) (7).

Functional segments associated with the mature molecule include a type I collagen binding domain (aa 19‑46), two non‑RGD cell binding sites (aa 30‑57 and 261‑281), an RGD alpha v beta 3 integrin‑binding site (aa 286‑288) and two potential hydroxyapatite (HAp) nucleation domains (aa 76‑83 and 151‑158) (3, 4, 8‑11). HAp formation requires a BSP nucleation site composed of at least eight consecutive glutamic acid residues and, likely, a contribution from a BSP‑associated co‑nucleator (10, 12). BSP is highly glycosylated, sulfated, and phosphorylated. Phosphorylation may impact HAp growth, while carbohydrate may regulate cell adhesion (1, 3, 13). Mature human BSP is 70%, 72%, 78%, and 72% aa identical to porcine, rat, canine, and mouse BSP, respectively. BSP is synthesized by megakaryocytes/platelets, osteoblasts, osteocytes, odontoblasts, osteoclasts, and bone marrow stromal cells (14‑17).

  1. Qin, C. et al. (2004) Crit. Rev. Oral Biol. Med. 15:126.
  2. Alford, A.I. and K.D. Hankenson (2006) Bone 38:749.
  3. Ganss, B. et al. (1999) Crit. Rev. Oral Biol. Med. 10:79.
  4. Fisher, L.W. et al. (1990) J. Biol. Chem. 265:2347.
  5. Kerr, J.M. et al. (1993) Genomics 17:408.
  6. Kim, R.H. et al. (1994) Matrix Biol. 14:31.
  7. Zaia, J. et al. (2001) Biochemistry 40:12983.
  8. Tye, C.E. et al. (2005) J. Biol. Chem. 280:13487.
  9. Stubbs, J.T. et al. (1997) J. Bone Miner. Res. 12:1210.
  10. Tye, C.E. et al. (2003) J. Biol. Chem. 278:7949.
  11. Miyauchi, A. et al. (1991) J. Biol. Chem. 266:20369.
  12. Hakki, S.S. et al. (2006) J. Periodontol. 77:167.
  13. Wazen, R.M. et al. (2007) J. Histochem. Cytochem. 55:35.
  14. Kacena, M.A. et al. (2006) Bone 39:978.
  15. Bianco, P. et al. (1991) Calcif. Tissue Int. 49:421.
  16. Chen, J. et al. (1992) J. Bone Miner. Res. 7:987.
  17. Kreke, M.R. et al. (2005) Bone 36:1047.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IBSP/Sialoprotein II Antibody (AF4014)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Binding Assay.


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Bioinformatics

Gene Symbol IBSP
Uniprot