IBRDC2 Antibody (OTI9H10) [CoraFluor™ 1]

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI9H10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

IBRDC2 Antibody (OTI9H10) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-256 of human RNF144B (NP_877434) produced in E. coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: RNF144B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for IBRDC2 Antibody (OTI9H10) [CoraFluor™ 1]

• bA528A10.3
• E3 ubiquitin-protein ligase RNF144B
• EC 6.3.2
• EC 6.3.2.-
• IBR domain containing 2
• IBR domain-containing protein 2
• IBRDC2
• KIAA0161
• p53-inducible RING finger protein
• p53RFP
• PIR2
• ring finger protein 144BMGC71786

Background

E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, therebypromoting their degradation. Induces apoptosis via a TP53/p53-dependent but caspase-independent mechanism

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RNF144B