IBRDC2 Antibody (4F1) - Azide and BSA Free

Western Blot: IBRDC2 Antibody (4F1) [H00255488-M02] - IBRDC2 monoclonal antibody (M02), clone 4F1 Analysis of IBRDC2 expression in IMR-32.

Immunocytochemistry/Immunofluorescence: IBRDC2 Antibody (4F1) [H00255488-M02] - Analysis of monoclonal antibody to IBRDC2 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: IBRDC2 Antibody (4F1) [H00255488-M02] - Analysis of monoclonal antibody to IBRDC2 on formalin-fixed paraffin-embedded human cerebellum. Antibody concentration 1.2 ug/ml.

Western Blot: IBRDC2 Antibody (4F1) [H00255488-M02] - Analysis of RNF144B expression in transfected 293T cell line by IBRDC2 monoclonal antibody (M02), clone 4F1.Lane 1: RNF144B transfected lysate(33.6 KDa).Lane 2: Non-transfected lysate.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 4F1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

IBRDC2 Antibody (4F1) - Azide and BSA Free Summary

• Immunogen: IBRDC2 (NP_877434, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGSAGRLHYLAMTAENPTPGDLAPAPLITCKLCLCEQSLDKMTTLQECQCIFCTACLKQYMQLAIREGCGSPITCPDMVCLNHGTLQEAEIACLVPVDQF
• Specificity: IBRDC2 (4F1)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: RNF144B
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate for Western Blot. Has also been used for immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for IBRDC2 Antibody (4F1) - Azide and BSA Free

• bA528A10.3
• E3 ubiquitin-protein ligase RNF144B
• EC 6.3.2
• EC 6.3.2.-
• IBR domain containing 2
• IBR domain-containing protein 2
• IBRDC2
• KIAA0161
• p53-inducible RING finger protein
• p53RFP
• PIR2
• ring finger protein 144BMGC71786

Background

E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, therebypromoting their degradation. Induces apoptosis via a TP53/p53-dependent but caspase-independent mechanism

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RNF144B
• Entrez:
  • 255488(Human)
• Uniprot:
  • NP_877434(Human)