Hyaluronidase 1/HYAL1 Antibody (1N3B1)

Western Blot: Hyaluronidase 1/HYAL1 Antibody (1N3B1) [NBP3-33459] - Western blot analysis of various lysates, using Hyaluronidase 1/HYAL1 Rabbit mAb at 1:1500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Negative control (NC): MOLT-4Exposure time: 60s.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 1N3B1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Hyaluronidase 1/HYAL1 Antibody (1N3B1) Summary

• Description: Novus Biologicals Rabbit Hyaluronidase 1/HYAL1 Antibody (1N3B1) (NBP3-33459) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-435 of human Hyaluronidase 1/HYAL1.2).
  
  Sequence:
  FRGPLLPNRPFTTVWNANTQWCLERHGVDVDVSVFDVVANPGQTFRGPDMTIFYSSQLGTYPYYTPTGEPVFGGLPQNASLIAHLARTFQDILAAIPAPDFSGLAVIDWEAWRPRWAFNWDTKDIYRQRSRALVQAQHPDWPAPQVEAVAQDQFQGAARAWMAGTLQLGRALRPRGLWGFYGFPDCYNYDFLSPNYTGQCPSGIRAQNDQLGWLWGQSRALYPSIYMPAVLEGTGKSQMYVQHRVAEAFRVAVAAGDPNLPVLPYVQIFYDTTNHFLPLDELEHSLGESAAQGAAGVVLWVSWENTRTKESCQAIKEYMDTTLGPFILNVTSGALLCSQALCSGHGRCVRRTSHPKALLLLNPASFSIQLTPGGGPLSLRGALSLEDQAQMAVEFKCRCYPGWQAPWCERKSMW
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HYAL1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 ug/mL
  • Western Blot 1:1000 - 1:5000
• Theoretical MW: 10 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for Hyaluronidase 1/HYAL1 Antibody (1N3B1)

• EC 3.2.1.35
• FUS2
• HYAL1
• HYAL-1
• Hyaluronidase 1
• Hyaluronoglucosaminidase 1
• hyaluronoglucosaminidase-1
• LUCA1
• luCa-1
• LUCA1hyaluronidase-1
• Lung carcinoma protein 1
• MGC45987
• NAT6
• plasma hyaluronidase
• tumor suppressor LUCA-1

Background

HYAL1 codes for a protein that may have a role in promoting tumor progression and blocking the TGFB1-enhanced cell growth, and is expressed highly in the liver, kidney, and heart, with weaker expression in the lung placenta, and skeletal muscle. There are seven isoforms of HYAL1, with the longest being isoform 1, which is only expressed in bladder and prostate cancer cells and is 435 amino acids long and weighs approximately 48 kDa. Current studies are being done on several diseases and disorders relating to this gene including lung carcinoma, lysosomal storage disease, diabetes mellitus, ovarian cancer, endometrial carcinoma, prostate cancer, and breast cancer. HYAL1 has also been shown to have interactions with GUSB, ARSB, IDUA, FAM107B, and COL2A1 in pathways such as the metabolic, lysosome, and Maroteaux-Lamy syndrome pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HYAL1