CACNA2D2 Antibody (4E3) - Azide and BSA Free

Western Blot: CACNA2D2 Antibody (4E3) [H00009254-M12] - Analysis of CACNA2D2 expression in HeLa(Cat # L013V1).

Immunocytochemistry/Immunofluorescence: CACNA2D2 Antibody (4E3) [H00009254-M12] - Analysis of monoclonal antibody to CACNA2D2 on HeLa cell. Antibody concentration 10 ug/ml

Sandwich ELISA: CACNA2D2 Antibody (4E3) [H00009254-M12] - Detection limit for recombinant GST tagged CACNA2D2 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 4E3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CACNA2D2 Antibody (4E3) - Azide and BSA Free Summary

• Immunogen: CACNA2D2 (NP_006021, 65 a.a. ~ 162 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PQQHTMQHWARRLEQEVDGVMRIFGGVQQLREIYKDNRNLFEVQENEPQKLVEKVAGDIESLLDRKVQALKRLADAAENFQKAHRWQDNIKEEDIVYY
• Specificity: Reacts with calcium channel, voltage-dependent, alpha 2/delta subunit 2.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CACNA2D2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot
• Application Notes: This antibody is reactive against cell lysate in western blot and recombinant protein in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CACNA2D2 Antibody (4E3) - Azide and BSA Free

• alpha 2 delta calcium channel subunit
• CACNA2D
• CACNA2D2
• calcium channel, voltage-dependent, alpha 2/delta subunit 2
• gene 26
• KIAA0558
• KIAA0558Voltage-gated calcium channel subunit alpha-2/delta-2
• LUAC11.1
• voltage-dependent calcium channel subunit alpha-2/delta-2

Background

This gene encodes a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CACNA2D2 Antibody (H00009254-M12)(2)

Publications using H00009254-M12

• Warnier M, Roudbaraki M, Derouiche S et al. CACNA2D2 promotes tumorigenesis by stimulating cell proliferation and angiogenesis. Oncogene 2015-10-16 [PMID: 25619833]
• Pippucci T, Parmeggiani A, Palombo F et al. A Novel Null Homozygous Mutation ConfirmsCACNA2D2 as a Gene Mutated in Epileptic Encephalopathy. PLoS One. 2013-12-16 [PMID: 24358150]

Bioinformatics

• Gene Symbol: CACNA2D2
• Entrez:
  • 9254(Human)
• Uniprot:
  • NP_006021(Human)