Human Total Legumain DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Total Legumain DuoSet ELISA, 15 Plate Summary

• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: LGMN

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Total Legumain DuoSet ELISA, 15 Plate

• AEP
• Asparaginyl Endopeptidase
• cysteine protease 1
• Legumain
• LGMN
• LGMN1
• Protease, cysteine 1
• protease, cysteine, 1 (legumain)
• PRSC1EC 3.4.22.34

Background

Legumain is a lysosomal cysteine protease that plays a pivotal role in the endosomal/lysosomal degradation system. Legumain deficiency causes the accumulation of pro-Cathepsins B, H and L, another group of lysosomal cysteine proteases. Overexpression of Legumain in tumors is significant for invasion/metastasis. Also known as Asparaginyl Endopeptidase, it specifically cleaves peptide bonds with Asn or Asp at the P1 position.

Publications for Legumain/Asparaginyl Endopeptidase (DY4769)(5)

Publications using DY4769

• AS Clees, V Stolp, B Häupl, DC Fuhrmann, F Wempe, M Seibert, S Weber, A Banning, R Tikkanen, R Williams, B Brüne, H Serve, F Schnütgen, I von Metzle, N Kurrle Identification of the Cysteine Protease Legumain as a Potential Chronic Hypoxia-Specific Multiple Myeloma Target Gene Cells, 2022-01-15;11(2):. 2022-01-15 [PMID: 35053409] (Human)
• NN Lunde, MH Haugen, KB Bodin Lars, I Damgaard, SJ Pettersen, R Kasem, W Rut, M Drag, M Poreba, HT Johansen, R Solberg Glycosylation is important for legumain localization and processing to active forms but not for cystatin E/M inhibitory functions Biochimie, 2017-05-17;0(0):. 2017-05-17 [PMID: 28528272] (Human)
• Y Cui, Q Li, H Li, Y Wang, H Wang, W Chen, S Zhang, J Cao, T Liu Asparaginyl endopeptidase improves the resistance of microtubule-targeting drugs in gastric cancer through IQGAP1 modulating the EGFR/JNK/ERK signaling pathway Onco Targets Ther, 2017-02-03;10(0):627-643. 2017-02-03 [PMID: 28223821] (Human)
• A Dutta, DN Potier, MJ Walker, OJ Gray, C Parker, M Holland, AJ Williamson, A Pierce, RD Unwin, S Krishnan, V Saha, AD Whetton Development of a selected reaction monitoring mass spectrometry-based assay to detect asparaginyl endopeptidase activity in biological fluids Oncotarget, 2016-10-25;7(43):70822-70831. 2016-10-25 [PMID: 27683124] (Human)
• Pitteri SJ, JeBailey L, Faca VM, Thorpe JD, Silva MA, Ireton RC, Horton MB, Wang H, Pruitt LC, Zhang Q, Cheng KH, Urban N, Hanash SM, Dinulescu DM Integrated proteomic analysis of human cancer cells and plasma from tumor bearing mice for ovarian cancer biomarker discovery. PLoS ONE, 2009-11-19;4(11):e7916. 2009-11-19 [PMID: 19936259] (Human)

Bioinformatics

• Gene Symbol: LGMN