Human TIMP-2 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human TIMP-2 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: TIMP2

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human TIMP-2 DuoSet ELISA, 15 Plate

• CSC-21Ktissue inhibitor of metalloproteinase 2
• metalloproteinase inhibitor 2
• TIMP metallopeptidase inhibitor 2
• TIMP2
• TIMP-2
• Tissue inhibitor of metalloproteinases 2

Background

TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.

Publications for TIMP-2 (DY971)(19)

Publications using DY971

• de Wijs, CJ;Streng, LWJM;Stolker, RJ;Ter Horst, M;Hoorn, EJ;Mahtab, EAF;Mik, EG;Harms, FA; Mitochondrial oxygenation monitoring and acute kidney injury risk in cardiac surgery: A prospective cohort study Journal of clinical anesthesia 2024-12-09 [PMID: 39657393] (Human)
• Xu, S;Hultström, M;Larsson, A;Lipcsey, M;Lindskog, C;Bülow, S;Frithiof, R;Venge, P; The Human Phospholipase B-II Precursor (HPLBII-P) in Urine as a Novel Biomarker of Glomerular Activity in COVID-19 and Diabetes Mellitus Journal of clinical medicine 2024-04-26 [PMID: 38731071] (Human)
• Janev, A;Ramuta, TŽ;Jerman, UD;Obradovi?, H;Kamenšek, U;?emažar, M;Kreft, ME; Human amniotic membrane inhibits migration and invasion of muscle-invasive bladder cancer urothelial cells by downregulating the FAK/PI3K/Akt/mTOR signalling pathway Scientific reports 2023-11-06 [PMID: 37932474] (Human)
• Kartasheva-Ebertz D, Gaston J, Lair-Mehiri L, Mottez E, Buivan T, Massault P, Scatton O, Gaujoux S, Vaillant J, Pol S, Lagaye S IL-17A in Human Liver: Significant Source of Inflammation and Trigger of Liver Fibrosis Initiation. Int J Mol Sci, 2022-08-29;23(17):. 2022-08-29 [PMID: 36077175] (Human)
• H Huang, Q Lin, X Dai, J Chen, Z Bai, X Li, F Fang, Y Li Derivation and validation of urinary TIMP-1 for the prediction of acute kidney injury and mortality in critically ill children Journal of Translational Medicine, 2022-02-23;20(1):102. 2022-02-23 [PMID: 35197070] (Human)
• S Boumiza, K Chahed, Z Tabka, MP Jacob, X Norel, G Ozen MMPs and TIMPs levels are correlated with anthropometric parameters, blood pressure, and endothelial function in obesity Scientific Reports, 2021-10-08;11(1):20052. 2021-10-08 [PMID: 34625635] (Human)
• A Abbes, Y Zayani, W Zidi, MB Hammami, A Mebazaa, D El Euch, A Ben Ammar, H Sanhaji, MV El May, M Mokni, M Feki, M Allal-Elas Matrix metalloproteinase-7 could be a predictor for acute inflammation in psoriatic patients Cytokine, 2020-07-11;134(0):155195. 2020-07-11 [PMID: 32663776] (Human)
• CC Wu, YR Chen, DH Lu, LH Hsu, KC Yang, S Sumi Evaluation of the post-treatment anti-inflammatory capacity of osteoarthritic chondrocytes: An in�vitro study using baicalein Regen Ther, 2020-02-24;14(0):177-183. 2020-02-24 [PMID: 32128354] (Human)
• TT Vuong, SB Rønning, TAE Ahmed, K Brathagen, V Høst, MT Hincke, HP Suso, ME Pedersen Processed eggshell membrane powder regulates cellular functions and increase MMP-activity important in early wound healing processes PLoS ONE, 2018-08-06;13(8):e0201975. 2018-08-06 [PMID: 30080894] (Human)
• S Brilha, CWM Ong, B Weksler, N Romero, PO Couraud, JS Friedland Matrix metalloproteinase-9 activity and a downregulated Hedgehog pathway impair blood-brain barrier function in an in vitro model of CNS tuberculosis Sci Rep, 2017-11-22;7(1):16031. 2017-11-22 [PMID: 29167512] (Human)
• CW Ong, PJ Pabisiak, S Brilha, P Singh, F Roncaroli, PT Elkington, JS Friedland Complex regulation of neutrophil-derived MMP-9 secretion in central nervous system tuberculosis J Neuroinflammation, 2017-02-07;14(1):31. 2017-02-07 [PMID: 28173836] (Human)
• Rath T, Zwaschka L, Hage L, Kugler M, Menendez K, Naehrlich L, Schulz R, Roderfeld M, Roeb E Identification of neutrophil activation markers as novel surrogate markers of CF lung disease. PLoS ONE, 2014-12-29;9(12):e115847. 2014-12-29 [PMID: 25545245] (Human)
• Gomez I, Benyahia C, Louedec L, Leseche G, Jacob M, Longrois D, Norel X Decreased PGE(2) content reduces MMP-1 activity and consequently increases collagen density in human varicose vein. PLoS ONE, 2014-02-05;9(2):e88021. 2014-02-05 [PMID: 24505358]
• Tsai HC, Ye SY, Kunin CM, Lee SS, Wann SR, Tai MH, Shi MH, Liu YC, Chen YS Expression of matrix metalloproteinases and their tissue inhibitors in the serum and cerebrospinal fluid of patients with HIV-1 infection and syphilis or neurosyphilis. Cytokine, 2011-02-26;54(0):109-16. 2011-02-26 [PMID: 21354815] (Human)
• Shyamsundar M, McKeown ST, O'Kane CM, O'Kane CM, O'Kane CM, O'Kane CM, O'Kane CM, Craig TR, Brown V, Thickett DR, Matthay MA, Taggart CC, Backman JT, Elborn JS, McAuley DF Simvastatin decreases lipopolysaccharide-induced pulmonary inflammation in healthy volunteers. Am. J. Respir. Crit. Care Med., 2009-03-26;179(12):1107-14. 2009-03-26 [PMID: 19324974] (Human)
• Sheen P, O'Kane CM, O'Kane CM, Chaudhary K, Tovar M, Santillan C, Sosa J, Caviedes L, Gilman RH, Stamp G, Friedland JS High MMP-9 activity characterises pleural tuberculosis correlating with granuloma formation. Eur. Respir. J., 2008-08-20;33(1):134-41. 2008-08-20 [PMID: 18715875] (Human)
• Dietmann A, Helbok R, Lackner P, Issifou S, Lell B, Matsiegui PB, Reindl M, Schmutzhard E, Kremsner PG Matrix metalloproteinases and their tissue inhibitors (TIMPs) in Plasmodium falciparum malaria: serum levels of TIMP-1 are associated with disease severity. J. Infect. Dis., 2008-06-01;197(11):1614-20. 2008-06-01 [PMID: 18700258] (Human)
• Tsai HC, Chung LY, Chen ER, Sy CL Association of matrix metalloproteinase-9 and tissue inhibitors of metalloproteinase-4 in cerebrospinal fluid with blood-brain barrier dysfunction in patients with eosinophilic meningitis caused by Angiostrongylus cantonensis. Am. J. Trop. Med. Hyg., 2008-01-01;78(1):20-7. 2008-01-01 [PMID: 18187780] (Human)
• Bergin PJ, Anders E, Sicheng W, Erik J, Jennie A, Hans L, Pierre M, Qiang PH, Marianne QJ Increased production of matrix metalloproteinases in Helicobacter pylori-associated human gastritis. Helicobacter, 2004-06-01;9(0):201. 2004-06-01 [PMID: 15165255] (Human)

FAQs for TIMP-2 (DY971). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TIMP2