Human TIMP-1 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human TIMP-1 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: TIMP1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human TIMP-1 DuoSet ELISA, 15 Plate

• CLGI
• Collagenase inhibitor
• collagenase inhibitor)
• EPATIMP-1
• EPO
• erythroid potentiating activity
• Erythroid-potentiating activity
• Fibroblast collagenase inhibitor
• FLJ90373
• HCI
• metalloproteinase inhibitor 1
• TIMP metallopeptidase inhibitor 1
• TIMP1
• TIMP-1
• TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity
• Tissue inhibitor of metalloproteinases 1

Background

TIMPs-1 through -4 regulate the activity of zinc metalloproteases known as MMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin- like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation of MMP-2 by MMP-14.

Publications for TIMP-1 (DY970)(47)

Publications using DY970

• Fallahi, F;Askari, N;Jamali, T;Parsapour, S;Ghasemi, H;Shams, J;Yaraee, R;Ghazanfari, Z;Ghazanfari, T; MMP-9 and TIMPs profiles in sulfur mustard-exposed individuals with serious lung complications International immunopharmacology 2024-12-09 [PMID: 39657535] (Human)
• Almuntashiri, S;Dutta, S;Zhu, Y;Gamare, S;Ramírez, G;Irineo-Moreno, V;Camarena, A;Regino, N;Campero, P;Hernández-Cardenas, CM;Rodriguez-Reyna, TS;Zuñiga, J;Owen, CA;Wang, X;Zhang, D; Estrogen-dependent gene regulation: Molecular basis of TIMP-1 as a sex-specific biomarker for acute lung injury Physiological reports 2024-09-01 [PMID: 39267201] (Human)
• Zisman, D;Sabtan, H;Rahat, MM;Simanovich, E;Haddad, A;Gazitt, T;Feld, J;Slobodin, G;Kibari, A;Elias, M;Rahat, MA; Tofacitinib Regulates Endostatin via Effects on CD147 and Cathepsin S International journal of molecular sciences 2024-07-02 [PMID: 39000375] (Human)
• Cherpaz, M;Meugnier, E;Seillier, G;Pozzi, M;Pierrard, R;Leboube, S;Farhat, F;Vola, M;Obadia, JF;Amaz, C;Chalabreysse, L;May, C;Chanon, S;Brun, C;Givre, L;Bidaux, G;Mewton, N;Derumeaux, G;Bergerot, C;Paillard, M;Thibault, H; Myocardial transcriptomic analysis of diabetic patients with aortic stenosis: key role for mitochondrial calcium signaling Cardiovascular diabetology 2024-07-08 [PMID: 38978010] (Human)
• Calver, J;Parmar, N;Harris, G;Lithgo, R;Stylianou, P;Zetterberg, F;Gooptu, B;MacKinnon, A;Carr, S;Borthwick, L;Scott, D;Stewart, I;Slack, R;Jenkins, G;John, A; Defining the mechanism of galectin-3-mediated TGF-?1 activation and its role in lung fibrosis Journal of Biological Chemistry 2024-04-18 [PMID: 38641066] (Human)
• Wierzbicka-Ruci?ska, A;Kubiszewska, I;Grzywa-Czuba, R;Gackowska, L;Szalecki, M;Micha?kiewicz, J;Trojanek, JB; Implications of the Matrix Metalloproteinases, Their Tissue Inhibitors and Some Other Inflammatory Mediators Expression Levels in Children Obesity-Related Phenotypes Journal of personalized medicine 2024-03-19 [PMID: 38541059] (Human)
• Coufal, S;Kverka, M;Kreisinger, J;Thon, T;Rob, F;Kolar, M;Reiss, Z;Schierova, D;Kostovcikova, K;Roubalova, R;Bajer, L;Jackova, Z;Mihula, M;Drastich, P;Tresnak Hercogova, J;Novakova, M;Vasatko, M;Lukas, M;Tlaskalova-Hogenova, H;Jiraskova Zakostelska, Z; Serum TGF-?1 and CD14 Predicts Response to Anti-TNF-? Therapy in IBD Journal of immunology research 2023-06-20 [PMID: 37383609] (Human)
• Buchheit, T;Huh, Y;Breglio, A;Bang, S;Xu, J;Matsuoka, Y;Guo, R;Bortsov, A;Reinecke, J;Wehling, P;Jun Huang, T;Ji, RR; Intrathecal Administration of Conditioned Serum from Different Species Resolves Chemotherapy-Induced Neuropathic Pain in Mice via Secretory Exosomes Brain, behavior, and immunity 2023-05-05 [PMID: 37150265] (Human)
• D Abasheva, MM Dolcet-Neg, MA Fernández-, JM Mora-Gutié, J Orbe, FJ Escalada, N Garcia-Fer Association between Circulating Levels of 25-Hydroxyvitamin D3 and Matrix Metalloproteinase-10 (MMP-10) in Patients with Type 2 Diabetes Nutrients, 2022-08-24;14(17):. 2022-08-24 [PMID: 36079742] (Human)
• Kartasheva-Ebertz D, Gaston J, Lair-Mehiri L, Mottez E, Buivan T, Massault P, Scatton O, Gaujoux S, Vaillant J, Pol S, Lagaye S IL-17A in Human Liver: Significant Source of Inflammation and Trigger of Liver Fibrosis Initiation. Int J Mol Sci, 2022-08-29;23(17):. 2022-08-29 [PMID: 36077175] (Human)
• S Rutecki, P Szulc, M Paku?a, P Uruski, A Radziemski, E Naumowicz, R Moszy?ski, A Tykarski, J Miku?a-Pie, K Ksi??ek Pro-cancerogenic effects of spontaneous and drug-induced senescence of ovarian cancer cells in vitro and in vivo: a comparative analysis Journal of ovarian research, 2022-07-26;15(1):87. 2022-07-26 [PMID: 35883110] (Human)
• JP Lapuente, G Gómez, J Marco-Brua, P Fernández, P Desportes, J Sanz, M García-Gil, F Bermejo, JV San Martín, A Algaba, JC De Gregori, D Lapuente, A De Gregori, B Lapuente, S Gómez, MLV Andrés, A Anel Evaluation in a Cytokine Storm Model In Vivo of the Safety and Efficacy of Intravenous Administration of PRS CK STORM (Standardized Conditioned Medium Obtained by Coculture of Monocytes and Mesenchymal Stromal Cells) Biomedicines, 2022-05-08;10(5):. 2022-05-08 [PMID: 35625831] (Human)
• P Globig, R Madurawala, R Willumeit-, F Martini, E Mazzoni, BJC Luthringer Mg-based materials diminish tumor spreading and cancer metastases Bioactive materials, 2022-05-10;19(0):594-610. 2022-05-10 [PMID: 35600975] (Human)
• S Cambier, M Metzemaeke, AC Carvalho, A Nooyens, C Jacobs, L Vanderbeke, B Malengier-, M Gouwy, E Heylen, P Meersseman, G Hermans, E Wauters, A Wilmer, C Consortium, D Schols, P Matthys, G Opdenakker, RE Marques, J Wauters, J Vandooren, P Proost Atypical response to bacterial co-infection and persistent neutrophilic broncho-alveolar inflammation distinguish critical COVID-19 from influenza JCI Insight, 2022-01-11;0(0):. 2022-01-11 [PMID: 34793331] (Human)
• S Boumiza, K Chahed, Z Tabka, MP Jacob, X Norel, G Ozen MMPs and TIMPs levels are correlated with anthropometric parameters, blood pressure, and endothelial function in obesity Scientific Reports, 2021-10-08;11(1):20052. 2021-10-08 [PMID: 34625635] (Human)
• Chris D. Hermann, Benjamin Schoeps, Celina Eckfeld, Enkhtsetseg Munkhbaatar, Lukas Kniep, Olga Prokopchuk et al. TIMP1 expression underlies sex disparity in liver metastasis and survival in pancreatic cancer Journal of Experimental Medicine 2021-11-01 [PMID: 34533565] (Human)
• K Noda, B Dufner, H Ito, K Yoshida, G Balboni, RH Straub Differential inflammation-mediated function of prokineticin 2 in the synovial fibroblasts of patients with rheumatoid arthritis compared with osteoarthritis Scientific Reports, 2021-09-15;11(1):18399. 2021-09-15 [PMID: 34526577] (Human)
• Y Hosokawa, I Hosokawa, K Ozaki Nobiletin Decreases Inflammatory Mediator Expression in Tumor Necrosis Factor-Stimulated Human Periodontal Ligament Cells Mediators of Inflammation, 2021-07-10;2021(0):5535844. 2021-07-10 [PMID: 34335088] (Human)
• S Hong, KH Park, YE Lee, S Shin, HJ Kim, YM Kim Antibody microarray analysis of the amniotic fluid proteome�for predicting the outcome of rescue cerclage in patients with cervical insufficiency Bioscience Reports, 2021-07-30;41(7):. 2021-07-30 [PMID: 34195783] (Human)
• R Urbanavi?i, R Zabitait?, A Kriš?iukai, VP Deltuva, D Skiriut? Serum protein triplet TGF-&beta1, TIMP-1, and YKL-40 serve as diagnostic and prognostic profile for astrocytoma Scientific Reports, 2021-06-23;11(1):13100. 2021-06-23 [PMID: 34162919] (Human)
• MM Brunton-O', AS Holley, KE Hally, GA Kristono, SA Harding, PD Larsen A combined biomarker approach for characterising extracellular matrix profiles in acute myocardial infarction Scientific Reports, 2021-06-16;11(1):12705. 2021-06-16 [PMID: 34135421] (Human)
• C Veraar, J Kliman, A Benazzo, F Oberndorfe, M Laggner, P Hacker, T Raunegger, S Janik, P Jaksch, W Klepetko, HJ Ankersmit, B Moser Potential novel biomarkers for chronic lung allograft dysfunction and azithromycin responsive allograft dysfunction Scientific Reports, 2021-03-24;11(1):6799. 2021-03-24 [PMID: 33762606] (Human)
• Y Hosokawa, I Hosokawa, K Ozaki, T Matsuo The Polymethoxy Flavonoid Sudachitin Inhibits Interleukin-1&beta-Induced Inflammatory Mediator Production in Human Periodontal Ligament Cells BioMed Research International, 2021-01-28;2021(0):8826586. 2021-01-28 [PMID: 33575345] (Human)
• HK Pihlstrøm, T Ueland, AE Michelsen, P Aukrust, F Gatti, C Hammarströ, M Kasprzycka, J Wang, G Haraldsen, G Mjøen, DO Dahle, K Midtvedt, IA Eide, A Hartmann, H Holdaas Exploring the potential effect of paricalcitol on markers of inflammation in de novo renal transplant recipients PLoS ONE, 2020-12-16;15(12):e0243759. 2020-12-16 [PMID: 33326471] (Human)
• JB Trojanek, J Micha?kiew, R Grzywa-Czu, W Ja?czyk, L Gackowska, I Kubiszewsk, A Helmin-Bas, A Wierzbicka, M Szalecki, P Socha Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in Peripheral Blood Leukocytes and Plasma of Children with Nonalcoholic Fatty Liver Disease Mediators Inflamm., 2020-09-10;2020(0):8327945. 2020-09-10 [PMID: 32963496] (Human)
• A Abbes, Y Zayani, W Zidi, MB Hammami, A Mebazaa, D El Euch, A Ben Ammar, H Sanhaji, MV El May, M Mokni, M Feki, M Allal-Elas Matrix metalloproteinase-7 could be a predictor for acute inflammation in psoriatic patients Cytokine, 2020-07-11;134(0):155195. 2020-07-11 [PMID: 32663776] (Human)
• CC Wu, YR Chen, DH Lu, LH Hsu, KC Yang, S Sumi Evaluation of the post-treatment anti-inflammatory capacity of osteoarthritic chondrocytes: An in�vitro study using baicalein Regen Ther, 2020-02-24;14(0):177-183. 2020-02-24 [PMID: 32128354] (Human)
• JM Mora-Gutié, JA Rodríguez, MA Fernández-, J Orbe, FJ Escalada, MJ Soler, MF Slon Roble, M Riera, JA Páramo, N Garcia-Fer MMP-10 is Increased in Early Stage Diabetic Kidney Disease and can be Reduced by Renin-Angiotensin System Blockade Sci Rep, 2020-01-08;10(1):26. 2020-01-08 [PMID: 31913319] (Human)
• YJ Park, SJ Woo, YM Kim, S Hong, YE Lee, KH Park Immune and Inflammatory Proteins in Cord Blood as Predictive Biomarkers of Retinopathy of Prematurity in Preterm Infants Invest. Ophthalmol. Vis. Sci., 2019-09-03;60(12):3813-3820. 2019-09-03 [PMID: 31525777] (Human)
• S Coufal, N Galanova, L Bajer, Z Gajdarova, D Schierova, Z Jiraskova, K Kostovciko, Z Jackova, Z Stehlikova, P Drastich, H Tlaskalova, M Kverka Inflammatory Bowel Disease Types Differ in Markers of Inflammation, Gut Barrier and in Specific Anti-Bacterial Response Cells, 2019-07-13;8(7):. 2019-07-13 [PMID: 31337064] (Human)
• R Linder, E Rönmark, J Pourazar, AF Behndig, A Blomberg, A Lindberg Proteolytic biomarkers are related to prognosis in COPD- report from a population-based cohort Respir. Res., 2018-04-12;19(1):64. 2018-04-12 [PMID: 29650051] (Human)
• O Prokopchuk, B Grünwald, U Nitsche, C Jäger, OL Prokopchuk, EC Schubert, H Friess, ME Martignoni, A Krüger Elevated systemic levels of the matrix metalloproteinase inhibitor TIMP-1 correlate with clinical markers of cachexia in patients with chronic pancreatitis and pancreatic cancer BMC Cancer, 2018-02-02;18(1):128. 2018-02-02 [PMID: 29394913] (Human)
• S Brilha, CWM Ong, B Weksler, N Romero, PO Couraud, JS Friedland Matrix metalloproteinase-9 activity and a downregulated Hedgehog pathway impair blood-brain barrier function in an in vitro model of CNS tuberculosis Sci Rep, 2017-11-22;7(1):16031. 2017-11-22 [PMID: 29167512] (Human)
• CW Ong, PJ Pabisiak, S Brilha, P Singh, F Roncaroli, PT Elkington, JS Friedland Complex regulation of neutrophil-derived MMP-9 secretion in central nervous system tuberculosis J Neuroinflammation, 2017-02-07;14(1):31. 2017-02-07 [PMID: 28173836] (Human)
• Shams-Un-Nisa Naveed MMP-1 Activation Contributes to Airway Smooth Muscle Growth and Asthma Severity Am. J. Respir. Crit. Care Med, 2017-04-15;0(0):. 2017-04-15 [PMID: 27967204] (Human)
• Association of Matrix Metalloproteinases -7, -8 and -9 and TIMP -1 with Disease Severity in Acute Pancreatitis. A Cohort Study PLoS ONE, 2016-08-25;11(8):e0161480. 2016-08-25 [PMID: 27561093] (Human)
• Linder R, Ronmark E, Pourazar J, Behndig A, Blomberg A, Lindberg A Serum metalloproteinase-9 is related to COPD severity and symptoms - cross-sectional data from a population based cohort-study. Respir Res, 2015-02-21;16(0):28. 2015-02-21 [PMID: 25849664] (Human)
• Rath T, Zwaschka L, Hage L, Kugler M, Menendez K, Naehrlich L, Schulz R, Roderfeld M, Roeb E Identification of neutrophil activation markers as novel surrogate markers of CF lung disease. PLoS ONE, 2014-12-29;9(12):e115847. 2014-12-29 [PMID: 25545245] (Human)
• Ji J, von Scheele I, Bergstrom J, Billing B, Dahlen B, Lantz A, Larsson K, Palmberg L Compartment differences of inflammatory activity in chronic obstructive pulmonary disease. Respir Res, 2014-08-26;15(0):104. 2014-08-26 [PMID: 25155252] (Human)
• Gomez I, Benyahia C, Louedec L, Leseche G, Jacob M, Longrois D, Norel X Decreased PGE(2) content reduces MMP-1 activity and consequently increases collagen density in human varicose vein. PLoS ONE, 2014-02-05;9(2):e88021. 2014-02-05 [PMID: 24505358] (Human)
• Tsai HC, Ye SY, Kunin CM, Lee SS, Wann SR, Tai MH, Shi MH, Liu YC, Chen YS Expression of matrix metalloproteinases and their tissue inhibitors in the serum and cerebrospinal fluid of patients with HIV-1 infection and syphilis or neurosyphilis. Cytokine, 2011-02-26;54(0):109-16. 2011-02-26 [PMID: 21354815] (Human)
• O'Kane CM, Elkington PT, Jones MD, Caviedes L, Tovar M, Gilman RH, Stamp G, Friedland JS STAT3, p38 MAPK, and NF-kappaB drive unopposed monocyte-dependent fibroblast MMP-1 secretion in tuberculosis. Am. J. Respir. Cell Mol. Biol., 2009-11-13;43(0):465. 2009-11-13 [PMID: 19915152] (Human)
• Shyamsundar M, McKeown ST, O'Kane CM, O'Kane CM, O'Kane CM, O'Kane CM, O'Kane CM, Craig TR, Brown V, Thickett DR, Matthay MA, Taggart CC, Backman JT, Elborn JS, McAuley DF Simvastatin decreases lipopolysaccharide-induced pulmonary inflammation in healthy volunteers. Am. J. Respir. Crit. Care Med., 2009-03-26;179(12):1107-14. 2009-03-26 [PMID: 19324974] (Human)
• Dietmann A, Helbok R, Lackner P, Issifou S, Lell B, Matsiegui PB, Reindl M, Schmutzhard E, Kremsner PG Matrix metalloproteinases and their tissue inhibitors (TIMPs) in Plasmodium falciparum malaria: serum levels of TIMP-1 are associated with disease severity. J. Infect. Dis., 2008-06-01;197(11):1614-20. 2008-06-01 [PMID: 18700258] (Human)
• Tsai HC, Chung LY, Chen ER, Sy CL Association of matrix metalloproteinase-9 and tissue inhibitors of metalloproteinase-4 in cerebrospinal fluid with blood-brain barrier dysfunction in patients with eosinophilic meningitis caused by Angiostrongylus cantonensis. Am. J. Trop. Med. Hyg., 2008-01-01;78(1):20-7. 2008-01-01 [PMID: 18187780] (Human)
• Lauder SN, Carty SM, Carpenter CE Interleukin-1beta induced activation of nuclear factor-kappab can be inhibited by novel pharmacological agents in osteoarthritis. Rheumatology (Oxford), 2007-01-11;46(5):752-8. 2007-01-11 [PMID: 17218328] (Human)
• Bergin PJ, Anders E, Sicheng W, Erik J, Jennie A, Hans L, Pierre M, Qiang PH, Marianne QJ Increased production of matrix metalloproteinases in Helicobacter pylori-associated human gastritis. Helicobacter, 2004-06-01;9(0):201. 2004-06-01 [PMID: 15165255] (Human)

FAQs for TIMP-1 (DY970). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TIMP1