Human Serum Albumin Antibody (3B8-H3-G1) - Chimeric - BSA Free

Immunofluorescent staining of HepG2 cells using NBP3-52356 (green). DAPI nuclear stain (blue) shows cell nuclei. This antibody specifically stains the endogenous Human Serum Albumin protein in the cytoplasm of HepG2 cells.

Western blotting of 2 μL human serum with NBP3-52356, detected with anti-chicken-HRP.This antibody recognizes endogenous albumin protein in human serum at ~66 kDa.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 3B8-H3-G1
• Clonality: Monoclonal
• Host: Chicken
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Human Serum Albumin Antibody (3B8-H3-G1) - Chimeric - BSA Free Summary

• Description: This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone 3B8-H3-G1 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone 3B8-H3-G1 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Human Serum Albumin
• Isotype: IgY
• Clonality: Monoclonal
• Host: Chicken
• Gene: ALB
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Western Blot 1:1000-1:3000
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1X PBS (pH 7.4)
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Affinity purified

Alternate Names for Human Serum Albumin Antibody (3B8-H3-G1) - Chimeric - BSA Free

• albumin
• cell growth inhibiting protein 42
• DKFZp779N1935
• growth-inhibiting protein 20
• PRO0883
• PRO0903
• PRO1341
• serum albumin

Background

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Mutations in this gene on chromosome 4 result in various anomalous proteins. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. The human albumin gene is 16,961 nucleotides long from the putative 'cap' site to the first poly(A) addition site. It is split into 15 exons which are symmetrically placed within the 3 domains that are thought to have arisen by triplication of a single primordial domain. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ALB