Human MICA DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human MICA DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: MICA

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human MICA DuoSet ELISA, 15 Plate

• FLJ60820
• MGC111087
• MICA
• PERB11.1

Background

MHC class I chain-related genes MICA and MICB are transmembrane glycoproteins that function as ligands for human NKG2D. The two proteins are highly related, sharing 85% amino acid identity, but are also polymorphic. Recognition of MICA or MICB by NKG2D results in the activation of NK cell cytolytic activity and/or cytokine production. MICA/B are minimally expressed on normal cells, but are frequently expressed on or shed from epithelial tumors and can be induced by bacterial and viral infections. MICA and MICB recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.

Publications for MICA (DY1300)(34)

Publications using DY1300

• Pogoda-Weso?owska, A;?wi?tek, A;Synowiec, A;Zdanowski, R;Stachura, I;Szewczuk, A;Pietras, E;St?pie?, A; The role of selected NKG2DLs such as MICA, MICB and ULBP4 as potential markers in multiple sclerosis Scientific reports 2025-07-29 [PMID: 40730869] (Human)
• Kowash, RR;Sabnani, M;Gray, LT;Deng, Q;Girard, L;Naito, Y;Masuhiro, K;Minna, JD;Gerber, DE;Koyama, S;Liu, ZL;Baruah, H;Akbay, EA; A novel and potent MICA/B antibody is therapeutically effective in KRAS LKB1 mutant lung cancer models bioRxiv : the preprint server for biology 2024-07-31 [PMID: 39211152] (Human)
• Seller, A;Tegeler, CM;Mauermann, J;Schreiber, T;Hagelstein, I;Liebel, K;Koch, A;Heitmann, JS;Greiner, SM;Hayn, C;Dannehl, D;Engler, T;Hartkopf, AD;Hahn, M;Brucker, SY;Salih, HR;Märklin, M; Soluble NKG2DLs Are Elevated in Breast Cancer Patients and Associate with Disease Outcome International journal of molecular sciences 2024-04-08 [PMID: 38612935] (Human)
• Wang, CM;Tan, KP;Wu, YJ;Zheng, JW;Wu, J;Chen, JY; Functional MICA Variants Are Differentially Associated with Immune-Mediated Inflammatory Diseases International journal of molecular sciences 2024-03-06 [PMID: 38474281] (Human)
• MJ Lee, MW Leong, A Rustagi, A Beck, L Zeng, S Holmes, LS Qi, CA Blish SARS-CoV-2 escapes direct NK cell killing through Nsp1-mediated downregulation of ligands for NKG2D Cell Reports, 2022-12-12;41(13):111892. 2022-12-12 [PMID: 36543165] (Human)
• K Masuhiro, M Tamiya, K Fujimoto, S Koyama, Y Naito, A Osa, T Hirai, H Suzuki, N Okamoto, T Shiroyama, K Nishino, Y Adachi, T Nii, Y Kinugasa-K, A Kajihara, T Morita, S Imoto, S Uematsu, T Irie, D Okuzaki, T Aoshi, Y Takeda, T Kumagai, T Hirashima, A Kumanogoh Bronchoalveolar lavage fluid reveals factors contributing to the efficacy of PD-1 blockade in lung cancer JCI Insight, 2022-05-09;7(9):. 2022-05-09 [PMID: 35389889] (Human)
• D Samarkanov, S Cox, D Hernandez, L Rodriguez, ML Pérez, A Madrigal, A Vilarrodon, S Querol, RP Casaroli-M Cord blood and amniotic membrane extract eye drop preparations display immune-suppressive and regenerative properties Scientific Reports, 2021-07-02;11(1):13754. 2021-07-02 [PMID: 34215804] (Human)
• CM Wang, KP Tan, YJ Jan Wu, JC Lin, JW Zheng, AL Yu, JM Wu, JY Chen MICA*019 Allele and Soluble MICA as Biomarkers for Ankylosing Spondylitis in Taiwanese Journal of personalized medicine, 2021-06-16;11(6):. 2021-06-16 [PMID: 34208618] (Human)
• X Wu, Y Zhang, Y Li, IGH Schmidt-Wo Increase of Antitumoral Effects of Cytokine-Induced Killer Cells by Antibody-Mediated Inhibition of MICA Shedding Cancers (Basel), 2020-07-07;12(7):. 2020-07-07 [PMID: 32645836] (Human)
• DP Muñoz, SM Yannone, A Daemen, Y Sun, F Vakar-Lope, M Kawahara, AM Freund, F Rodier, JD Wu, PY Desprez, DH Raulet, PS Nelson, LJ van 't Vee, J Campisi, JP Coppé Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging JCI Insight, 2019-06-11;5(0):. 2019-06-11 [PMID: 31184599] (Human)
• MJ Sakiyama, I Espinoza, A Reddy, F de Carlo, A Kumar, AS Levenson, S Bae, X Zhou, PP Claudio, J Lewin, V Manucha, CR Pound, S Vijayakuma, CR Gomez Race-associated expression of MHC class I polypeptide-related sequence A (MICA) in prostate cancer Exp. Mol. Pathol., 2019-04-17;0(0):. 2019-04-17 [PMID: 31004600] (Human)
• JL Chen, CC Chang, YS Huang, HY Kuo, TY Chen, CW Wang, SH Kuo, YL Lin Persistently elevated soluble MHC class I polypeptide-related sequence A and transforming growth factor-?1 levels are poor prognostic factors in head and neck squamous cell carcinoma after definitive chemoradiotherapy PLoS ONE, 2018-08-10;13(8):e0202224. 2018-08-10 [PMID: 30096190] (Human)
• XY Fan, PY Wang, C Zhang, YL Zhang, Y Fu, C Zhang, QX Li, JN Zhou, BE Shan, DW He All-trans retinoic acid enhances cytotoxicity of CIK cells against human lung adenocarcinoma by upregulating MICA and IL-2 secretion Sci Rep, 2017-11-28;7(1):16481. 2017-11-28 [PMID: 29184163] (Human)
• H Ghadially, L Brown, C Lloyd, L Lewis, A Lewis, J Dillon, R Sainson, J Jovanovic, NJ Tigue, D Bannister, L Bamber, V Valge-Arch, RW Wilkinson MHC class I chain-related protein A and B (MICA and MICB) are predominantly expressed intracellularly in tumour and normal tissue Br. J. Cancer, 2017-03-23;0(0):. 2017-03-23 [PMID: 28334733] (Human)
• Genetics Variants and Serum Levels of MHC Class I Chain-related A in Predicting Hepatocellular Carcinoma Development in Chronic Hepatitis C Patients Post Antiviral Treatment EBioMedicine, 2016-12-01;0(0):. 2016-12-01 [PMID: 27998720] (Human)
• M Ke, H Wang, Y Zhou, J Li, Y Liu, M Zhang, J Dou, T Xi, B Shen, C Zhou SEP enhanced the antitumor activity of 5-fluorouracil by up-regulating NKG2D/MICA and reversed immune suppression via inhibiting ROS and caspase-3 in mice Oncotarget, 2016-08-02;7(31):49509-49526. 2016-08-02 [PMID: 27385218] (Human)
• The MICA-129 dimorphism affects NKG2D signaling and outcome of hematopoietic stem cell transplantation EMBO Mol Med, 2015-11-01;7(11):1480-502. 2015-11-01 [PMID: 26483398] (Human)
• Zingoni A, Cecere F, Vulpis E, Fionda C, Molfetta R, Soriani A, Petrucci M, Ricciardi M, Fuerst D, Amendola M, Mytilineos J, Cerboni C, Paolini R, Cippitelli M, Santoni A Genotoxic Stress Induces Senescence-Associated ADAM10-Dependent Release of NKG2D MIC Ligands in Multiple Myeloma Cells. J Immunol, 2015-06-12;195(2):736-48. 2015-06-12 [PMID: 26071561] (Human)
• Cox S, Laza-Briviesca R, Pearson H, Soria B, Gibson D, Gomez S, Madrigal J, Saudemont A Umbilical cord blood plasma contains soluble NKG2D ligands that mediate loss of natural killer cell function and cytotoxicity. Eur J Immunol, 2015-06-09;45(8):2324-34. 2015-06-09 [PMID: 25991034] (Human)
• Gonzalez-Foruria I, Santulli P, Chouzenoux S, Carmona F, Batteux F, Chapron C Soluble ligands for the NKG2D receptor are released during endometriosis and correlate with disease severity. PLoS ONE, 2015-03-16;10(3):e0119961. 2015-03-16 [PMID: 25775242] (Human)
• Ullrich N, Heinemann A, Nilewski E, Scheffrahn I, Klode J, Scherag A, Schadendorf D, Singer B, Helfrich I CEACAM1-3S Drives Melanoma Cells into NK Cell-Mediated Cytolysis and Enhances Patient Survival. Cancer Res, 2015-03-05;75(9):1897-907. 2015-03-05 [PMID: 25744717] (Human)
• Dambrauskas Z, Svensson H, Joshi M, Hyltander A, Naredi P, Iresjo B Expression of major histocompatibility complex class I-related chain A/B (MICA/B) in pancreatic carcinoma. Int J Oncol, 2013-10-31;44(1):99-104. 2013-10-31 [PMID: 24173243] (Human)
• Giuliani M, Bennaceur-Griscelli A, Nanbakhsh A, Oudrhiri N, Chouaib S, Azzarone B, Durrbach A, Lataillade J TLR ligands stimulation protects MSC from NK killing. Stem Cells, 2014-01-01;32(1):290-300. 2014-01-01 [PMID: 24123639] (Human)
• Reiners K, Kessler J, Sauer M, Rothe A, Hansen H, Reusch U, Hucke C, Kohl U, Durkop H, Engert A, von Strandmann E Rescue of impaired NK cell activity in hodgkin lymphoma with bispecific antibodies in vitro and in patients. Mol Ther, 2013-03-05;21(4):895-903. 2013-03-05 [PMID: 23459515] (Human)
• Kumar V, Yi Lo P, Sawai H, Kato N, Takahashi A, Deng Z, Urabe Y, Mbarek H, Tokunaga K, Tanaka Y, Sugiyama M, Mizokami M, Muroyama R, Tateishi R, Omata M, Koike K, Tanikawa C, Kamatani N, Kubo M, Nakamura Y, Matsuda K Soluble MICA and a MICA variation as possible prognostic biomarkers for HBV-induced hepatocellular carcinoma. PLoS ONE, 2012-09-14;7(9):e44743. 2012-09-14 [PMID: 23024757] (Human)
• Yamanegi K, Yamane J, Kobayashi K Valproic acid cooperates with hydralazine to augment the susceptibility of human osteosarcoma cells to Fas- and NK cell-mediated cell death. Int. J. Oncol., 2012-04-19;41(1):83-91. 2012-04-19 [PMID: 22576685] (Human)
• Mamessier E, Sylvain A, Thibult ML, Houvenaeghel G, Jacquemier J, Castellano R, Goncalves A, Andre P, Romagne F, Thibault G, Viens P, Birnbaum D, Bertucci F, Moretta A, Olive D Human breast cancer cells enhance self tolerance by promoting evasion from NK cell antitumor immunity. J. Clin. Invest., 2011-08-15;121(9):3609-22. 2011-08-15 [PMID: 21841316] (Human)
• Kumar V, Kato N, Urabe Y, Takahashi A, Muroyama R, Hosono N, Otsuka M, Tateishi R, Omata M, Nakagawa H, Koike K, Kamatani N, Kubo M, Nakamura Y, Matsuda K Genome-wide association study identifies a susceptibility locus for HCV-induced hepatocellular carcinoma. Nat. Genet., 2011-04-17;43(5):455-8. 2011-04-17 [PMID: 21499248] (Human)
• Muntasell A, Magri G, Pende D, Angulo A, Lopez-Botet M Inhibition of NKG2D expression in NK cells by cytokines secreted in response to human cytomegalovirus infection. Blood, 2010-04-14;115(25):5170-9. 2010-04-14 [PMID: 20393128] (Human)
• Stanzer S, Janesch B, Resel M, Augustin T, Samonigg H, Bauernhofer T The role of activation-induced cell death in the higher onset of spontaneous apoptosis of NK cell subsets in patients with metastatic epithelial cancer. Cell. Immunol., 2009-11-26;261(2):99-104. 2009-11-26 [PMID: 20035932] (Human)
• Ho VT, Vanneman M, Kim H, Sasada T, Kang YJ, Pasek M, Cutler C, Koreth J, Alyea E, Sarantopoulos S, Antin JH, Ritz J, Canning C, Kutok J, Mihm MC, Dranoff G, Soiffer R Biologic activity of irradiated, autologous, GM-CSF-secreting leukemia cell vaccines early after allogeneic stem cell transplantation. Proc. Natl. Acad. Sci. U.S.A., 2009-08-26;106(37):15825-30. 2009-08-26 [PMID: 19717467] (Human)
• Abe Y, Muto M, Nieda M, Nakagawa Y, Nicol A, Kaneko T, Goto S, Yokokawa K, Suzuki K Clinical and immunological evaluation of zoledronate-activated Vgamma9gammadelta T-cell-based immunotherapy for patients with multiple myeloma. Exp. Hematol., 2009-05-04;37(8):956-68. 2009-05-04 [PMID: 19409955] (Human)
• Cerboni C, Ardolino M, Santoni A, Zingoni A Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells. Blood, 2009-01-05;113(13):2955-64. 2009-01-05 [PMID: 19124832] (Human)
• Siemens DR, Hu N, Sheikhi AK, Chung E, Frederiksen LJ, Pross H, Graham CH Hypoxia increases tumor cell shedding of MHC class I chain-related molecule: role of nitric oxide. Cancer Res., 2008-06-15;68(12):4746-53. 2008-06-15 [PMID: 18559521] (Human)

FAQs for MICA (DY1300). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: MICA