Human IL-36 alpha/IL-1F6 DuoSet ELISA, 5 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human IL-36 alpha/IL-1F6 DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL36A

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-36 alpha/IL-1F6 DuoSet ELISA, 5 Plate

• FIL1 epsilon
• FIL1
• FIL1(EPSILON)
• FIL1E
• IL-1 epsilon
• IL1(EPSILON)
• IL1E
• IL-1E
• IL-1F6 (FIL-1-epsilon)
• IL1F6
• IL-1F6
• IL36 alpha
• IL-36 alpha
• IL36A
• interleukin 1 family, member 6 (epsilon)
• interleukin 1, epsilon
• Interleukin 36, Alpha
• Interleukin-1 epsilon
• interleukin-1 family member 6
• Interleukin-36 Alpha

Background

Interleukin 36 alpha (IL-36 alpha), also known as IL-1F6 and FIL1 epsilon, is a member of the IL-1 family of proteins. Cells reported to express IL-36 alpha include monocytes, B cells, and T cells. Notably, IL-36 alpha is the only novel IL-1 family member expressed on T-cells. The receptor for IL-36 alpha is reported to be a combination of IL-1 R6/IL-1 R rp2 and IL-1 R3/IL-1 R AcP. Recombinant IL-36 alpha has been shown to activate pathways involving NF-kappa B and MAPK in an IL-1 R6/IL-1 R rp2-dependent manner.

Publications for IL-36 alpha/IL-1F6 (DY1078-05)(8)

Publications using DY1078-05

• S O'Reilly Interleukin-36alpha is elevated in diffuse systemic sclerosis and may potentiate fibrosis Cytokine, 2022-06-03;156(0):155921. 2022-06-03 [PMID: 35667282] (Human)
• JM Li, R Lu, Y Zhang, J Lin, X Hua, SC Pflugfelde, DQ Li IL-36alpha/IL-36RA/IL-38 signaling mediates inflammation and barrier disruption in human corneal epithelial cells under hyperosmotic stress The ocular surface, 2021-08-21;22(0):163-171. 2021-08-21 [PMID: 34428579] (Human)
• X Xie, H Hu, J He, Y Liu, F Guo, F Luo, M Jiang, L Wang Interleukin-36&alpha suppresses growth of non-small cell lung cancer in vitro by reducing angiogenesis FEBS Open Bio, 2021-05-02;11(5):1353-1363. 2021-05-02 [PMID: 33713575] (Human)
• X Gao, PKS Chan, GCY Lui, DSC Hui, IM Chu, X Sun, MS Tsang, BCL Chan, CW Lam, CK Wong Interleukin-38 ameliorates poly(I:C) induced lung inflammation: therapeutic implications in respiratory viral infections Cell Death & Disease, 2021-01-07;12(1):53. 2021-01-07 [PMID: 33414457] (Human)
• QX Zhang, Y Li, SM Jiang, LJ Zhang, M Yi, J Wang, Y Qi, L Yang, CS Yang Increased serum IL-36gamma levels are associated with disease severity in myasthenia gravis patients BMC Neurol, 2020-08-19;20(1):307. 2020-08-19 [PMID: 32814555] (Human)
• L Mercurio, CM Failla, L Capriotti, C Scarponi, F Facchiano, M Morelli, S Rossi, G Pagnanelli, C Albanesi, A Cavani, S Madonna Interleukin (IL)-17/IL-36 axis participates to the crosstalk between endothelial cells and keratinocytes during inflammatory skin responses PLoS ONE, 2020-04-30;15(4):e0222969. 2020-04-30 [PMID: 32352958] (Human)
• E Giannoudak, YE Hernandez-, K Mulfaul, SL Doyle, E Hams, PG Fallon, A Mat, D O'Shea, M Kopf, AE Hogan, PT Walsh Interleukin-36 cytokines alter the intestinal microbiome and can protect against obesity and metabolic dysfunction Nat Commun, 2019-09-05;10(1):4003. 2019-09-05 [PMID: 31488830] (Human)
• X Qin, M Liu, S Zhang, C Wang, T Zhang The role of IL-36? and its regulation in eosinophilic inflammation in allergic rhinitis Cytokine, 2019-03-01;117(0):84-90. 2019-03-01 [PMID: 30831444] (Human)

FAQs for IL-36 alpha/IL-1F6 (DY1078-05). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL36A