Human IL-3 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human IL-3 Quantikine ELISA Kit Summary

• Background: The Quantikine Human IL-3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-3 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-3 and antibodies raised against the recombinant factor. It has been shown to quantitate the recombinant factor accurately.
• Specificity: Natural and recombinant human IL-3
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL3

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-3 Quantikine ELISA Kit

• Hematopoietic growth factor
• IL3
• IL-3
• IL-3MGC79398
• interleukin 3 (colony-stimulating factor, multiple)
• interleukin-3
• Mast cell growth factor
• mast-cell growth factor
• MCGF
• MCGFMGC79399
• MULTI-CSF
• multilineage-colony-stimulating factor
• Multipotential colony-stimulating factor
• P-cell stimulating factor
• P-cell-stimulating factor

Background

Interleukin-3 (IL-3) is a secreted cytokine that stimulates the proliferation and differentiation of pluripotent hematopoietic stem cells and various lineage-committed progenitors. It also regulates the activation of mature mast cells, basophils, eosinophils, and macrophages. It is produced by activated T cells, thymic epithelial cells, activated mast cells, keratinocytes, neurons, and astrocytes. IL-3 signals through a receptor complex that contains IL-3 R alpha/CD123 and the common beta chain which is also a component of the receptors for IL-5 and GM-CSF. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils, and various myeloid leukemic cells. Human IL-3 does not show activity on mouse cells.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-3 (D3000)(17)

Publications using D3000

• KP Loh, JA Tooze, BJ Nicklas, SB Kritchevsk, JD Williamson, LR Ellis, BL Powell, TS Pardee, NG Goyal, HD Klepin Inflammatory biomarkers, geriatric assessment, and treatment outcomes in acute myeloid leukemia J Geriatr Oncol, 2019-04-05;0(0):. 2019-04-05 [PMID: 30962090] (Human)
• Almut Meyer-Bahl Human Basophils Modulate Plasma Cell Differentiation and Maturation J. Immunol., 2016-11-16;0(0):. 2016-11-16 [PMID: 27852746] (Human)
• Szygula Z, Lubkowska A, Giemza C, Skrzek A, Bryczkowska I, Dolegowska B Hematological parameters, and hematopoietic growth factors: EPO and IL-3 in response to whole-body cryostimulation (WBC) in military academy students. PLoS ONE, 2014-04-02;9(4):e93096. 2014-04-02 [PMID: 24695100] (Human)
• Zheng M, Zhang Z, Bemis K, Belch A, Pilarski L, Shively J, Kirshner J The systemic cytokine environment is permanently altered in multiple myeloma. PLoS ONE, 2013-03-27;8(3):e58504. 2013-03-27 [PMID: 23544044] (Human)
• Donhauser N, Pritschet K, Helm M, Harrer T, Schuster P, Ries M, Bischof G, Vollmer J, Smola S, Schmidt B Chronic immune activation in HIV-1 infection contributes to reduced interferon alpha production via enhanced CD40:CD40 ligand interaction. PLoS ONE, 2012-03-21;7(3):e33925. 2012-03-21 [PMID: 22470494] (Human)
• Sadeghi M, Daniel V, Schnitzler P, Lahdou I, Naujokat C, Zeier M, Opelz G Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria. Transplantation, 2009-11-15;88(9):1109-16. 2009-11-15 [PMID: 19898207] (Human)
• Chauhan D, Singh AV, Brahmandam M, Carrasco R, Bandi M, Hideshima T, Bianchi G, Podar K, Tai YT, Mitsiades C, Raje N, Jaye DL, Kumar SK, Richardson P, Munshi N, Anderson KC Functional interaction of plasmacytoid dendritic cells with multiple myeloma cells: a therapeutic target. Cancer Cell, 2009-10-06;16(4):309-23. 2009-10-06 [PMID: 19800576] (Human)
• Schuster C, Vaculik C, Fiala C, Meindl S, Brandt O, Imhof M, Stingl G, Eppel W, Elbe-Burger A HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells. J. Exp. Med., 2009-01-12;206(1):169-81. 2009-01-12 [PMID: 19139172] (Human)
• Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M Isolation and characterization of CD146+ multipotent mesenchymal stromal cells. Exp. Hematol., 2008-05-27;36(8):1035-46. 2008-05-27 [PMID: 18504067] (Human)
• Rudolph T, Schaps KP, Steven D, Koester R, Rudolph V, Berger J, Terres W, Meinertz T, Kaehler J Interleukin-3 is elevated in patients with coronary artery disease and predicts restenosis after percutaneous coronary intervention. Int. J. Cardiol., 2008-04-18;132(3):392-7. 2008-04-18 [PMID: 18378332] (Human)
• Sadeghi M, Susal C, Daniel V, Naujokat C, Zimmermann R, Huth-Kuhne A, Opelz G Short communication: decreasing soluble CD30 and increasing IFN-gamma plasma levels are indicators of effective highly active antiretroviral therapy. AIDS Res. Hum. Retroviruses, 2007-07-01;23(7):886-90. 2007-07-01 [PMID: 17678471] (Human)
• Hatfield K, Ryningen A, Corbascio M, Bruserud O Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts. Int. J. Cancer, 2006-11-15;119(10):2313-21. 2006-11-15 [PMID: 16921487] (Human)
• Vassina EM, Yousefi S, Simon D, Zwicky C, Conus S, Simon HU cIAP-2 and survivin contribute to cytokine-mediated delayed eosinophil apoptosis. Eur. J. Immunol., 2006-07-01;36(7):1975-84. 2006-07-01 [PMID: 16761316] (Human)
• Sadeghi M, Daniel V, Naujokat C, Wiesel M, Hergesell O, Opelz G Strong inflammatory cytokine response in male and strong anti-inflammatory response in female kidney transplant recipients with urinary tract infection. Transpl. Int., 2005-02-01;18(2):177-85. 2005-02-01 [PMID: 15691270] (Human)
• Drouet M, Mourcin F, Grenier N, Leroux V, Denis J, Mayol JF, Thullier P, Lataillade JJ, Herodin F Single administration of stem cell factor, FLT-3 ligand, megakaryocyte growth and development factor, and interleukin-3 in combination soon after irradiation prevents nonhuman primates from myelosuppression: long-term follow-up of hematopoiesis. Blood, 2003-10-02;103(3):878-85. 2003-10-02 [PMID: 14525791] (Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque))
• Bovolenta C, Pilotti E, Mauri M, Turci M, Ciancianaini P, Fisicaro P, Bertazzoni U, Poli G, Casoli C Human T-cell leukemia virus type 2 induces survival and proliferation of CD34(+) TF-1 cells through activation of STAT1 and STAT5 by secretion of interferon-gamma and granulocyte macrophage-colony-stimulating factor. Blood, 2002-01-01;99(1):224-31. 2002-01-01 [PMID: 11756175] (Human)
• Kadowaki N, Antonenko S, Lau JY, Liu YJ Natural interferon alpha/beta-producing cells link innate and adaptive immunity. J. Exp. Med., 2000-07-17;192(2):219-26. 2000-07-17 [PMID: 10899908] (Human)

FAQs for IL-3 (D3000). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL3