Human IL-3 Quantikine ELISA Kit

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

Human IL-3 Quantikine ELISA Kit Summary

Background
The Quantikine Human IL-3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-3 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-3 and antibodies raised against the recombinant factor. It has been shown to quantitate the recombinant factor accurately.
Specificity
Natural and recombinant human IL-3
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
IL3

Applications/Dilutions

Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using D3000.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-3 Quantikine ELISA Kit

  • Hematopoietic growth factor
  • IL3
  • IL-3
  • IL-3MGC79398
  • interleukin 3 (colony-stimulating factor, multiple)
  • interleukin-3
  • Mast cell growth factor
  • mast-cell growth factor
  • MCGF
  • MCGFMGC79399
  • MULTI-CSF
  • multilineage-colony-stimulating factor
  • Multipotential colony-stimulating factor
  • P-cell stimulating factor
  • P-cell-stimulating factor

Background

M-CSF, also known as CSF-1, is a four-alpha-helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. M-CSF is also essential for the survival and proliferation of osteoclast progenitors. M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis. M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta. Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells. The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur. Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen. Shorter transcripts encode M-CSF that lack cleavage and PG sites and produce an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer. Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor. The first 223 aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of dog, cow, mouse and rat M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-3 (D3000)(17)

We have publications tested in 1 confirmed species: Human.


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Human
(16)
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Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using D3000 Applications Species
KP Loh, JA Tooze, BJ Nicklas, SB Kritchevsk, JD Williamson, LR Ellis, BL Powell, TS Pardee, NG Goyal, HD Klepin Inflammatory biomarkers, geriatric assessment, and treatment outcomes in acute myeloid leukemia J Geriatr Oncol, 2019;0(0):. 2019 [PMID: 30962090] (Human) Human
Almut Meyer-Bahl Human Basophils Modulate Plasma Cell Differentiation and Maturation J. Immunol., 2016;0(0):. 2016 [PMID: 27852746] (Human) Human
Szygula Z, Lubkowska A, Giemza C, Skrzek A, Bryczkowska I, Dolegowska B Hematological parameters, and hematopoietic growth factors: EPO and IL-3 in response to whole-body cryostimulation (WBC) in military academy students. PLoS ONE, 2014;9(4):e93096. 2014 [PMID: 24695100] (Human) Human
Zheng M, Zhang Z, Bemis K, Belch A, Pilarski L, Shively J, Kirshner J The systemic cytokine environment is permanently altered in multiple myeloma. PLoS ONE, 2013;8(3):e58504. 2013 [PMID: 23544044] (Human) Human
Donhauser N, Pritschet K, Helm M, Harrer T, Schuster P, Ries M, Bischof G, Vollmer J, Smola S, Schmidt B Chronic immune activation in HIV-1 infection contributes to reduced interferon alpha production via enhanced CD40:CD40 ligand interaction. PLoS ONE, 2012;7(3):e33925. 2012 [PMID: 22470494] (Human) Human
Sadeghi M, Daniel V, Schnitzler P, Lahdou I, Naujokat C, Zeier M, Opelz G Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria. Transplantation, 2009;88(9):1109-16. 2009 [PMID: 19898207] (Human) Human
Chauhan D, Singh AV, Brahmandam M, Carrasco R, Bandi M, Hideshima T, Bianchi G, Podar K, Tai YT, Mitsiades C, Raje N, Jaye DL, Kumar SK, Richardson P, Munshi N, Anderson KC Functional interaction of plasmacytoid dendritic cells with multiple myeloma cells: a therapeutic target. Cancer Cell, 2009;16(4):309-23. 2009 [PMID: 19800576] (Human) Human
Schuster C, Vaculik C, Fiala C, Meindl S, Brandt O, Imhof M, Stingl G, Eppel W, Elbe-Burger A HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells. J. Exp. Med., 2009;206(1):169-81. 2009 [PMID: 19139172] (Human) Human
Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M Isolation and characterization of CD146+ multipotent mesenchymal stromal cells. Exp. Hematol., 2008;36(8):1035-46. 2008 [PMID: 18504067] (Human) Human
Rudolph T, Schaps KP, Steven D, Koester R, Rudolph V, Berger J, Terres W, Meinertz T, Kaehler J Interleukin-3 is elevated in patients with coronary artery disease and predicts restenosis after percutaneous coronary intervention. Int. J. Cardiol., 2009;132(3):392-7. 2009 [PMID: 18378332] (Human) Human
Show All 17 Publications.

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FAQs for IL-3 (D3000). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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Bioinformatics

Gene Symbol IL3