Human IL-27 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human IL-27 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL27

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-27 DuoSet ELISA, 15 Plate

• IL-27 p28 subunit
• IL-27 subunit alpha
• IL27
• IL-27
• IL27A
• IL-27-A
• IL27-A
• IL27p28
• IL30
• interleukin 27
• interleukin 30
• interleukin-27 subunit alpha
• MGC71873
• p28IL-27A

Background

IL-27 is a heterodimeric cytokine that is composed of EBI3 (which is related to the p40 subunit of IL-12 and IL-23) and p28 (which is related to the p35 chain of IL-12). IL-27 is expressed by monocytes, endothelial cells, and dendritic cells, and it signals through a receptor complex composed of IL-27 R alpha/WSX-1/TCCR and gp130. IL-27 induces IL-12 receptor expression on naïve CD4+ T cells, sensitizing them to subsequent IL-12 induced Th1 development. It also functions as an anti-inflammatory cytokine by limiting T and NKT cell activity.

Publications for IL-27 (DY2526)(38)

Publications using DY2526

• Balan, S;O'Brien, L;Peros, A;Wang, X;Leal-Rojas, I;Mcclain, C;Radford, KJ;Bhardwaj, N; Harnessing Notch Signaling to Enhance the Generation and Functionality of Human Conventional Type I Dendritic Cells for Cancer Immunotherapy Applications Cancer immunology research 2025-07-02 [PMID: 40600891] (Human)
• Zhu, T;Shen, Q;Shen, L;Wang, Y;Zhu, B;Ma, L;Feng, S;Wang, C;Yan, S;Li, J;Chen, Z;Zhou, J;Huang, H;Li, B;Shen, Z;Wang, Q;Wang, J;Gwinner, W;Scheffner, I;Rong, S;Yang, B;Wang, J;Haller, H;Han, X;Guo, G;Yin, Z;Jin, J;Lan, HY;Chen, J;Jiang, H; Senescence-induced p21high macrophages contributed to CD8+ T cells-related immune hyporesponsiveness in kidney transplantation via Zfp36/IL-27 axis Cell discovery 2025-04-15 [PMID: 40234384] (Human)
• Zhu, HZ;Niu, Y;Wen, JX;Yan, C;Cha, SN;Gao, Y;Hao, XL;Hou, WJ;Yan, L;Jiang, TW;Hu, ZD;Zheng, WQ; Accuracy of interleukin-27 in diagnosing tuberculous pleural effusion: Age should be considered Cytokine 2024-12-31 [PMID: 39742701] (Human)
• Pourfarzam, S;Ardestani, SK;Jamali, T;Ghazanfari, H;Naghizadeh, MM;Faghihzadeh, S;Yaraee, R;Ghazanfari, Z;Ghazanfari, T; Distinct inflammatory profiles in mustard lung: A study of sulfur mustard-exposed patients with serious pulmonary complications International immunopharmacology 2024-12-16 [PMID: 39689604] (Human)
• Martin, E;Winter, S;Garcin, C;Tanita, K;Hoshino, A;Lenoir, C;Fournier, B;Migaud, M;Boutboul, D;Simonin, M;Fernandes, A;Bastard, P;Le Voyer, T;Roupie, AL;Ben Ahmed, Y;Leruez-Ville, M;Burgard, M;Rao, G;Ma, CS;Masson, C;Soudais, C;Picard, C;Bustamante, J;Tangye, SG;Cheikh, N;Seppänen, M;Puel, A;Daly, M;Casanova, JL;Neven, B;Fischer, A;Latour, S; Role of IL-27 in Epstein-Barr virus infection revealed by IL-27RA deficiency Nature 2024-03-20 [PMID: 38509369] (Human)
• Pons, S;Trouillet-Assant, S;Subtil, F;Abbas-Chorfa, F;Cornaton, E;Berthiot, A;Galletti, S;Plat, A;Rapin, S;Trapes, L;Generenaz, L;Brengel-Pesce, K;Callies, A;Plaisant, F;Claris, O;Portefaix, A;Flamant, C;Butin, M; Performance of 11 Host Biomarkers Alone or in Combination in the Diagnosis of Late-Onset Sepsis in Hospitalized Neonates: The Prospective EMERAUDE Study Biomedicines 2023-06-13 [PMID: 37371798] (Human)
• YY Peng, SL Guo, XF Yan, LL Zhang, J Wang, GD Yuan, G Qing, LL Xu, Q Zhan Collective outbreak of severe acute histoplasmosis in immunocompetent Chinese in South America: the clinical characteristics and continuous monitoring of serum cytokines/chemokines BMC primary care, 2022-08-08;23(1):197. 2022-08-08 [PMID: 35934702] (Human)
• WJ Zhou, HL Yang, J Mei, KK Chang, H Lu, ZZ Lai, JW Shi, XH Wang, K Wu, T Zhang, J Wang, JS Sun, JF Ye, DJ Li, JY Zhao, LP Jin, MQ Li Fructose-1,6-bisphosphate prevents pregnancy loss by inducing decidual COX-2+ macrophage differentiation Science Advances, 2022-02-23;8(8):eabj2488. 2022-02-23 [PMID: 35196096] (Human)
• Y Qi, C Liu, X Zhong, X Ma, J Zhou, Y Shi, Y Yin, X Zhang, Y He, W Xu IL-27 as a potential biomarker for distinguishing between necrotising enterocolitis and highly suspected early-onset food protein-induced enterocolitis syndrome with abdominal gas signs EBioMedicine, 2021-10-07;72(0):103607. 2021-10-07 [PMID: 34628355] (Human)
• E Jover, L Matilla, M Garaikoetx, A Fernández-, P Muntendam, F Jaisser, P Rossignol, N López-Andr Beneficial Effects of Mineralocorticoid Receptor Pathway Blockade against Endothelial Inflammation Induced by SARS-CoV-2 Spike Protein Biomedicines, 2021-06-03;9(6):. 2021-06-03 [PMID: 34204890] (Human)
• TM Yendo, MN Sato, ACCC Branco, AJ Pietrobon, FME Teixeira, YÁL Ramos, RW Alberca, CG Valêncio, VN Arruda, R Romiti, M Arnone, ALDS Hirayama, AJDS Duarte, V Aoki, RL Orfali Impact of Inflammatory Immune Dysfunction in Psoriasis Patients at Risk for COVID-19 Vaccines, 2021-05-10;9(5):. 2021-05-10 [PMID: 34068473] (Human)
• B Min, D Kim, MJ Feige IL-30� (IL-27A): a familiar stranger in immunity, inflammation, and cancer Experimental & Molecular Medicine, 2021-05-28;53(5):823-834. 2021-05-28 [PMID: 34045653] (Human)
• N Dalil Roof, M Marjani, NK Dezfuli, P Tabarsi, A Moniri, M Varahram, IM Adcock, E Mortaz Potential diagnostic value of pleural fluid cytokines levels for tuberculous pleural effusion Scientific Reports, 2021-01-12;11(1):660. 2021-01-12 [PMID: 33436672] (Human)
• J Jeon, Y Lee, H Yu, UH Ha HSP70-Homolog DnaK of Pseudomonas aeruginosa Increases the Production of IL-27 through Expression of EBI3 via TLR4-Dependent NF-&kappaB and TLR4-Independent Akt Signaling International Journal of Molecular Sciences, 2020-12-02;21(23):. 2020-12-02 [PMID: 33276561] (Human)
• A Bobhate, V Viswanatha, V Aravindhan Anti-inflammatory cytokines IL-27, IL-10, IL-1Ra and TGF-&beta in subjects with increasing grades of glucose intolerence (DM-LTB-2) Cytokine, 2020-10-09;137(0):155333. 2020-10-09 [PMID: 33045524] (Human)
• L Moreira Ge, L de Oliveir, A da Silva J, L Furquim de, AK Felício Ba, AE Rizzato, P Trabasso, RL Mamoni, R Mendes Per, ML Cintra, L Nunes Sant, M de Carvalh, L Pereira Ru, MH de Souza L Association between IL-27 and Tr1 cells in severe form of paracoccidioidomycosis Cytokine, 2020-01-02;127(0):154962. 2020-01-02 [PMID: 31901599] (Human)
• K Moideen, NP Kumar, R Bethunaick, VV Banurekha, D Nair, S Babu Heightened systemic levels of anti-inflammatory cytokines in pulmonary tuberculosis and alterations following anti-tuberculosis treatment Cytokine, 2019-12-04;127(0):154929. 2019-12-04 [PMID: 31811994] (Human)
• M Ma, CM Percopo, DE Sturdevant, AC Sek, HD Komarow, HF Rosenberg Cytokine Diversity in Human Peripheral Blood Eosinophils: Profound Variability of IL-16 J. Immunol., 2019-06-10;0(0):. 2019-06-10 [PMID: 31182481] (Human)
• L Zhang, J Zhang, S Su, S Luo Changes in interleukin-27 levels in patients with acute coronary syndrome and their clinical significance PeerJ, 2019-01-04;7(0):e5652. 2019-01-04 [PMID: 30631648] (Human)
• ST Loughran, PA Power, PT Maguire, SL McQuaid, PJ Buchanan, I Jonsdottir, RW Newman, R Harvey, PA Johnson Influenza infection directly alters innate IL-23 and IL-12p70 and subsequent IL-17A and IFN-? responses to pneumococcus in vitro in human monocytes PLoS ONE, 2018-09-07;13(9):e0203521. 2018-09-07 [PMID: 30192848] (Human)
• A Busca, Y Konarski, N Gajanayaka, S O'Hara, J Angel, M Kozlowski, A Kumar cIAP1/2-TRAF2-SHP-1-Src-MyD88 Complex Regulates Lipopolysaccharide-Induced IL-27 Production through NF-?B Activation in Human Macrophages J. Immunol., 2018-01-22;0(0):. 2018-01-22 [PMID: 29358273] (Human)
• SY Nuñez, A Ziblat, F Secchiari, NI Torres, JM Sierra, XL Raffo Irao, RE Araya, CI Domaica, MB Fuertes, NW Zwirner Human M2 Macrophages Limit NK Cell Effector Functions through Secretion of TGF-? and Engagement of CD85j J. Immunol., 2017-12-27;0(0):. 2017-12-27 [PMID: 29282306] (Human)
• N Goli?, K Veljovi?, N Popovi?, J Djoki?, I Strahini?, I Mrvaljevi?, A Terzi?-Vid In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens BMC Microbiol., 2017-05-06;17(1):108. 2017-05-06 [PMID: 28477627] (Human)
• A Garg, R Trout, SA Spector Human Immunodeficiency Virus Type-1 Myeloid Derived Suppressor Cells Inhibit Cytomegalovirus Inflammation through Interleukin-27 and B7-H4 Sci Rep, 2017-03-24;7(0):44485. 2017-03-24 [PMID: 28338007] (Human)
• NS Hojo-Souza, DB Pereira, FS de Souza, TA de Oliveir, MS Cardoso, MS Tada, GM Zanini, DC Bartholome, RT Fujiwara, LL Bueno On the cytokine/chemokine network during Plasmodium vivax malaria: new insights to understand the disease Malar. J, 2017-01-24;16(1):42. 2017-01-24 [PMID: 28118834] (Human)
• IL-27 suppresses SKOV3 cells proliferation by enhancing STAT3 and inhibiting the Akt signal pathway Mol Immunol, 2016-09-15;78(0):155-163. 2016-09-15 [PMID: 27639718] (Human)
• A Malecka, Q Wang, S Shah, RV Sutavani, I Spendlove, JM Ramage, J Greensmith, HA Franks, MJ Gough, A Saalbach, PM Patel, AM Jackson Stromal fibroblasts support dendritic cells to maintain IL-23/Th17 responses after exposure to ionizing radiation J Leukoc Biol, 2016-04-05;0(0):. 2016-04-05 [PMID: 27049023] (Human)
• Teles R, Kelly-Scumpia K, Sarno E, Rea T, Ochoa M, Cheng G, Modlin R IL-27 Suppresses Antimicrobial Activity in Human Leprosy. J Invest Dermatol, 2015-06-01;135(10):2410-7. 2015-06-01 [PMID: 26030183] (Human)
• Guha R, Das S, Ghosh J, Sundar S, Dujardin J, Roy S Antimony resistant Leishmania donovani but not sensitive ones drives greater frequency of potent T-regulatory cells upon interaction with human PBMCs: role of IL-10 and TGF-beta in early immune response. PLoS Negl Trop Dis, 2014-07-17;8(7):e2995. 2014-07-17 [PMID: 25032977] (Human)
• Dietrich C, Candon S, Ruemmele F, Devergne O A soluble form of IL-27Ralpha is a natural IL-27 antagonist. J Immunol, 2014-04-25;192(11):5382-9. 2014-04-25 [PMID: 24771852] (Human)
• Zhang X, Tao Y, Chopra M, Ahn M, Marcus K, Choudhary N, Zhu H, Markovic-Plese S Differential reconstitution of T cell subsets following immunodepleting treatment with alemtuzumab (anti-CD52 monoclonal antibody) in patients with relapsing-remitting multiple sclerosis. J Immunol, 2013-11-06;191(12):5867-74. 2013-11-06 [PMID: 24198283] (Human)
• Lechner C, Komander K, Hegewald J, Huang X, Gantin R, Soboslay P, Agossou A, Banla M, Kohler C Cytokine and chemokine responses to helminth and protozoan parasites and to fungus and mite allergens in neonates, children, adults, and the elderly. Immun Ageing, 2013-07-15;10(1):29. 2013-07-15 [PMID: 23855879] (Human)
• Fitzgerald D, Fonseca-Kelly Z, Cullimore M, Safabakhsh P, Saris C, Zhang G, Rostami A Independent and interdependent immunoregulatory effects of IL-27, IFN-beta, and IL-10 in the suppression of human Th17 cells and murine experimental autoimmune encephalomyelitis. J Immunol, 2013-03-01;190(7):3225-34. 2013-03-01 [PMID: 23455508] (Human)
• Jiang S, Liu X, Luo L, Qu B, Huang X, Lin Y, Ye S, Liu Y Serum levels of Th17-related cytokines in Behcet disease patients after cataract surgery. Mol. Vis., 2011-05-31;17(0):1425-30. 2011-05-31 [PMID: 21655356] (Human)
• Sweeney CM, Lonergan R, Basdeo SA, Kinsella K, Dungan LS, Higgins SC, Kelly PJ, Costelloe L, Tubridy N, Mills KH, Fletcher JM IL-27 mediates the response to IFN-beta therapy in multiple sclerosis patients by inhibiting Th17 cells. Brain Behav. Immun., 2011-03-21;0(0):. 2011-03-21 [PMID: 21420486] (Human)
• Ramgolam VS, Sha Y, Marcus KL, Choudhary N, Troiani L, Chopra M, Markovic-Plese S B cells as a therapeutic target for IFN-beta in relapsing-remitting multiple sclerosis. J. Immunol., 2011-03-02;186(0):4518. 2011-03-02 [PMID: 21368231] (Human)
• Guzzo C, Hopman WM, Mat NF Impact of HIV infection, highly active antiretroviral therapy, and hepatitis C coinfection on serum interleukin-27. AIDS, 2010-06-01;24(9):1371-4. 2010-06-01 [PMID: 20375875] (Human)
• Correale J, Farez M Helminth antigens modulate immune responses in cells from multiple sclerosis patients through TLR2-dependent mechanisms. J. Immunol., 2009-10-07;183(9):5999-6012. 2009-10-07 [PMID: 19812189] (Human)

FAQs for IL-27 (DY2526). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: IL27