Human IL-27 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human IL-27 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL27

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-27 DuoSet ELISA, 15 Plate

• IL-27 p28 subunit
• IL-27 subunit alpha
• IL27
• IL-27
• IL27A
• IL-27-A
• IL27-A
• IL27p28
• IL30
• interleukin 27
• interleukin 30
• interleukin-27 subunit alpha
• MGC71873
• p28IL-27A

Background

IL-27 is a heterodimeric cytokine that is composed of EBI3 (which is related to the p40 subunit of IL-12 and IL-23) and p28 (which is related to the p35 chain of IL-12). IL-27 is expressed by monocytes, endothelial cells, and dendritic cells, and it signals through a receptor complex composed of IL-27 R alpha/WSX-1/TCCR and gp130. IL-27 induces IL-12 receptor expression on naïve CD4+ T cells, sensitizing them to subsequent IL-12 induced Th1 development. It also functions as an anti-inflammatory cytokine by limiting T and NKT cell activity.

Publications for IL-27 (DY2526)(38)

Publications using DY2526

• Balan, S;O'Brien, L;Peros, A;Wang, X;Leal-Rojas, I;Mcclain, C;Radford, KJ;Bhardwaj, N; Harnessing Notch Signaling to Enhance the Generation and Functionality of Human Conventional Type I Dendritic Cells for Cancer Immunotherapy Applications Cancer immunology research 2025-07-02 [PMID: 40600891] (Human)
• Zhu, T;Shen, Q;Shen, L;Wang, Y;Zhu, B;Ma, L;Feng, S;Wang, C;Yan, S;Li, J;Chen, Z;Zhou, J;Huang, H;Li, B;Shen, Z;Wang, Q;Wang, J;Gwinner, W;Scheffner, I;Rong, S;Yang, B;Wang, J;Haller, H;Han, X;Guo, G;Yin, Z;Jin, J;Lan, HY;Chen, J;Jiang, H; Senescence-induced p21high macrophages contributed to CD8+ T cells-related immune hyporesponsiveness in kidney transplantation via Zfp36/IL-27 axis Cell discovery 2025-04-15 [PMID: 40234384] (Human)
• Zhu, HZ;Niu, Y;Wen, JX;Yan, C;Cha, SN;Gao, Y;Hao, XL;Hou, WJ;Yan, L;Jiang, TW;Hu, ZD;Zheng, WQ; Accuracy of interleukin-27 in diagnosing tuberculous pleural effusion: Age should be considered Cytokine 2024-12-31 [PMID: 39742701] (Human)
• Pourfarzam, S;Ardestani, SK;Jamali, T;Ghazanfari, H;Naghizadeh, MM;Faghihzadeh, S;Yaraee, R;Ghazanfari, Z;Ghazanfari, T; Distinct inflammatory profiles in mustard lung: A study of sulfur mustard-exposed patients with serious pulmonary complications International immunopharmacology 2024-12-16 [PMID: 39689604] (Human)
• Martin, E;Winter, S;Garcin, C;Tanita, K;Hoshino, A;Lenoir, C;Fournier, B;Migaud, M;Boutboul, D;Simonin, M;Fernandes, A;Bastard, P;Le Voyer, T;Roupie, AL;Ben Ahmed, Y;Leruez-Ville, M;Burgard, M;Rao, G;Ma, CS;Masson, C;Soudais, C;Picard, C;Bustamante, J;Tangye, SG;Cheikh, N;Seppänen, M;Puel, A;Daly, M;Casanova, JL;Neven, B;Fischer, A;Latour, S; Role of IL-27 in Epstein-Barr virus infection revealed by IL-27RA deficiency Nature 2024-03-20 [PMID: 38509369] (Human)
• Pons, S;Trouillet-Assant, S;Subtil, F;Abbas-Chorfa, F;Cornaton, E;Berthiot, A;Galletti, S;Plat, A;Rapin, S;Trapes, L;Generenaz, L;Brengel-Pesce, K;Callies, A;Plaisant, F;Claris, O;Portefaix, A;Flamant, C;Butin, M; Performance of 11 Host Biomarkers Alone or in Combination in the Diagnosis of Late-Onset Sepsis in Hospitalized Neonates: The Prospective EMERAUDE Study Biomedicines 2023-06-13 [PMID: 37371798] (Human)
• YY Peng, SL Guo, XF Yan, LL Zhang, J Wang, GD Yuan, G Qing, LL Xu, Q Zhan Collective outbreak of severe acute histoplasmosis in immunocompetent Chinese in South America: the clinical characteristics and continuous monitoring of serum cytokines/chemokines BMC primary care, 2022-08-08;23(1):197. 2022-08-08 [PMID: 35934702] (Human)
• WJ Zhou, HL Yang, J Mei, KK Chang, H Lu, ZZ Lai, JW Shi, XH Wang, K Wu, T Zhang, J Wang, JS Sun, JF Ye, DJ Li, JY Zhao, LP Jin, MQ Li Fructose-1,6-bisphosphate prevents pregnancy loss by inducing decidual COX-2+ macrophage differentiation Science Advances, 2022-02-23;8(8):eabj2488. 2022-02-23 [PMID: 35196096] (Human)
• Y Qi, C Liu, X Zhong, X Ma, J Zhou, Y Shi, Y Yin, X Zhang, Y He, W Xu IL-27 as a potential biomarker for distinguishing between necrotising enterocolitis and highly suspected early-onset food protein-induced enterocolitis syndrome with abdominal gas signs EBioMedicine, 2021-10-07;72(0):103607. 2021-10-07 [PMID: 34628355] (Human)
• E Jover, L Matilla, M Garaikoetx, A Fernández-, P Muntendam, F Jaisser, P Rossignol, N López-Andr Beneficial Effects of Mineralocorticoid Receptor Pathway Blockade against Endothelial Inflammation Induced by SARS-CoV-2 Spike Protein Biomedicines, 2021-06-03;9(6):. 2021-06-03 [PMID: 34204890] (Human)
• TM Yendo, MN Sato, ACCC Branco, AJ Pietrobon, FME Teixeira, YÁL Ramos, RW Alberca, CG Valêncio, VN Arruda, R Romiti, M Arnone, ALDS Hirayama, AJDS Duarte, V Aoki, RL Orfali Impact of Inflammatory Immune Dysfunction in Psoriasis Patients at Risk for COVID-19 Vaccines, 2021-05-10;9(5):. 2021-05-10 [PMID: 34068473] (Human)
• B Min, D Kim, MJ Feige IL-30� (IL-27A): a familiar stranger in immunity, inflammation, and cancer Experimental & Molecular Medicine, 2021-05-28;53(5):823-834. 2021-05-28 [PMID: 34045653] (Human)
• N Dalil Roof, M Marjani, NK Dezfuli, P Tabarsi, A Moniri, M Varahram, IM Adcock, E Mortaz Potential diagnostic value of pleural fluid cytokines levels for tuberculous pleural effusion Scientific Reports, 2021-01-12;11(1):660. 2021-01-12 [PMID: 33436672] (Human)
• J Jeon, Y Lee, H Yu, UH Ha HSP70-Homolog DnaK of Pseudomonas aeruginosa Increases the Production of IL-27 through Expression of EBI3 via TLR4-Dependent NF-&kappaB and TLR4-Independent Akt Signaling International Journal of Molecular Sciences, 2020-12-02;21(23):. 2020-12-02 [PMID: 33276561] (Human)
• A Bobhate, V Viswanatha, V Aravindhan Anti-inflammatory cytokines IL-27, IL-10, IL-1Ra and TGF-&beta in subjects with increasing grades of glucose intolerence (DM-LTB-2) Cytokine, 2020-10-09;137(0):155333. 2020-10-09 [PMID: 33045524] (Human)
• L Moreira Ge, L de Oliveir, A da Silva J, L Furquim de, AK Felício Ba, AE Rizzato, P Trabasso, RL Mamoni, R Mendes Per, ML Cintra, L Nunes Sant, M de Carvalh, L Pereira Ru, MH de Souza L Association between IL-27 and Tr1 cells in severe form of paracoccidioidomycosis Cytokine, 2020-01-02;127(0):154962. 2020-01-02 [PMID: 31901599] (Human)
• K Moideen, NP Kumar, R Bethunaick, VV Banurekha, D Nair, S Babu Heightened systemic levels of anti-inflammatory cytokines in pulmonary tuberculosis and alterations following anti-tuberculosis treatment Cytokine, 2019-12-04;127(0):154929. 2019-12-04 [PMID: 31811994] (Human)
• M Ma, CM Percopo, DE Sturdevant, AC Sek, HD Komarow, HF Rosenberg Cytokine Diversity in Human Peripheral Blood Eosinophils: Profound Variability of IL-16 J. Immunol., 2019-06-10;0(0):. 2019-06-10 [PMID: 31182481] (Human)
• L Zhang, J Zhang, S Su, S Luo Changes in interleukin-27 levels in patients with acute coronary syndrome and their clinical significance PeerJ, 2019-01-04;7(0):e5652. 2019-01-04 [PMID: 30631648] (Human)
• ST Loughran, PA Power, PT Maguire, SL McQuaid, PJ Buchanan, I Jonsdottir, RW Newman, R Harvey, PA Johnson Influenza infection directly alters innate IL-23 and IL-12p70 and subsequent IL-17A and IFN-? responses to pneumococcus in vitro in human monocytes PLoS ONE, 2018-09-07;13(9):e0203521. 2018-09-07 [PMID: 30192848] (Human)
• A Busca, Y Konarski, N Gajanayaka, S O'Hara, J Angel, M Kozlowski, A Kumar cIAP1/2-TRAF2-SHP-1-Src-MyD88 Complex Regulates Lipopolysaccharide-Induced IL-27 Production through NF-?B Activation in Human Macrophages J. Immunol., 2018-01-22;0(0):. 2018-01-22 [PMID: 29358273] (Human)
• SY Nuñez, A Ziblat, F Secchiari, NI Torres, JM Sierra, XL Raffo Irao, RE Araya, CI Domaica, MB Fuertes, NW Zwirner Human M2 Macrophages Limit NK Cell Effector Functions through Secretion of TGF-? and Engagement of CD85j J. Immunol., 2017-12-27;0(0):. 2017-12-27 [PMID: 29282306] (Human)
• N Goli?, K Veljovi?, N Popovi?, J Djoki?, I Strahini?, I Mrvaljevi?, A Terzi?-Vid In vitro and in vivo antagonistic activity of new probiotic culture against Clostridium difficile and Clostridium perfringens BMC Microbiol., 2017-05-06;17(1):108. 2017-05-06 [PMID: 28477627] (Human)
• A Garg, R Trout, SA Spector Human Immunodeficiency Virus Type-1 Myeloid Derived Suppressor Cells Inhibit Cytomegalovirus Inflammation through Interleukin-27 and B7-H4 Sci Rep, 2017-03-24;7(0):44485. 2017-03-24 [PMID: 28338007] (Human)
• NS Hojo-Souza, DB Pereira, FS de Souza, TA de Oliveir, MS Cardoso, MS Tada, GM Zanini, DC Bartholome, RT Fujiwara, LL Bueno On the cytokine/chemokine network during Plasmodium vivax malaria: new insights to understand the disease Malar. J, 2017-01-24;16(1):42. 2017-01-24 [PMID: 28118834] (Human)
• IL-27 suppresses SKOV3 cells proliferation by enhancing STAT3 and inhibiting the Akt signal pathway Mol Immunol, 2016-09-15;78(0):155-163. 2016-09-15 [PMID: 27639718] (Human)
• A Malecka, Q Wang, S Shah, RV Sutavani, I Spendlove, JM Ramage, J Greensmith, HA Franks, MJ Gough, A Saalbach, PM Patel, AM Jackson Stromal fibroblasts support dendritic cells to maintain IL-23/Th17 responses after exposure to ionizing radiation J Leukoc Biol, 2016-04-05;0(0):. 2016-04-05 [PMID: 27049023] (Human)
• Teles R, Kelly-Scumpia K, Sarno E, Rea T, Ochoa M, Cheng G, Modlin R IL-27 Suppresses Antimicrobial Activity in Human Leprosy. J Invest Dermatol, 2015-06-01;135(10):2410-7. 2015-06-01 [PMID: 26030183] (Human)
• Guha R, Das S, Ghosh J, Sundar S, Dujardin J, Roy S Antimony resistant Leishmania donovani but not sensitive ones drives greater frequency of potent T-regulatory cells upon interaction with human PBMCs: role of IL-10 and TGF-beta in early immune response. PLoS Negl Trop Dis, 2014-07-17;8(7):e2995. 2014-07-17 [PMID: 25032977] (Human)
• Dietrich C, Candon S, Ruemmele F, Devergne O A soluble form of IL-27Ralpha is a natural IL-27 antagonist. J Immunol, 2014-04-25;192(11):5382-9. 2014-04-25 [PMID: 24771852] (Human)
• Zhang X, Tao Y, Chopra M, Ahn M, Marcus K, Choudhary N, Zhu H, Markovic-Plese S Differential reconstitution of T cell subsets following immunodepleting treatment with alemtuzumab (anti-CD52 monoclonal antibody) in patients with relapsing-remitting multiple sclerosis. J Immunol, 2013-11-06;191(12):5867-74. 2013-11-06 [PMID: 24198283] (Human)
• Lechner C, Komander K, Hegewald J, Huang X, Gantin R, Soboslay P, Agossou A, Banla M, Kohler C Cytokine and chemokine responses to helminth and protozoan parasites and to fungus and mite allergens in neonates, children, adults, and the elderly. Immun Ageing, 2013-07-15;10(1):29. 2013-07-15 [PMID: 23855879] (Human)
• Fitzgerald D, Fonseca-Kelly Z, Cullimore M, Safabakhsh P, Saris C, Zhang G, Rostami A Independent and interdependent immunoregulatory effects of IL-27, IFN-beta, and IL-10 in the suppression of human Th17 cells and murine experimental autoimmune encephalomyelitis. J Immunol, 2013-03-01;190(7):3225-34. 2013-03-01 [PMID: 23455508] (Human)
• Jiang S, Liu X, Luo L, Qu B, Huang X, Lin Y, Ye S, Liu Y Serum levels of Th17-related cytokines in Behcet disease patients after cataract surgery. Mol. Vis., 2011-05-31;17(0):1425-30. 2011-05-31 [PMID: 21655356] (Human)
• Sweeney CM, Lonergan R, Basdeo SA, Kinsella K, Dungan LS, Higgins SC, Kelly PJ, Costelloe L, Tubridy N, Mills KH, Fletcher JM IL-27 mediates the response to IFN-beta therapy in multiple sclerosis patients by inhibiting Th17 cells. Brain Behav. Immun., 2011-03-21;0(0):. 2011-03-21 [PMID: 21420486] (Human)
• Ramgolam VS, Sha Y, Marcus KL, Choudhary N, Troiani L, Chopra M, Markovic-Plese S B cells as a therapeutic target for IFN-beta in relapsing-remitting multiple sclerosis. J. Immunol., 2011-03-02;186(0):4518. 2011-03-02 [PMID: 21368231] (Human)
• Guzzo C, Hopman WM, Mat NF Impact of HIV infection, highly active antiretroviral therapy, and hepatitis C coinfection on serum interleukin-27. AIDS, 2010-06-01;24(9):1371-4. 2010-06-01 [PMID: 20375875] (Human)
• Correale J, Farez M Helminth antigens modulate immune responses in cells from multiple sclerosis patients through TLR2-dependent mechanisms. J. Immunol., 2009-10-07;183(9):5999-6012. 2009-10-07 [PMID: 19812189] (Human)

FAQs for IL-27 (DY2526). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL27