Human IL-1ra/IL-1F3 DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human IL-1ra/IL-1F3 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL1RN

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-1ra/IL-1F3 DuoSet ELISA, 15 Plate

• DIRA
• ICIL-1ra
• IL1F3
• IL-1F3
• IL1ra
• IL-1ra
• IL-1ra3
• IL1RN
• IL-1RN
• interleukin 1 receptor antagonist
• IRAP
• MVCD4

Background

Interleukin-1 receptor antagonist (IL-1ra; also known as IL-1F3) is a 22-25 kDa member of the IL-1 family of cytokines. Currently, there are 11 family members (IL-1F1-F11), nine of which form an IL-1 gene cluster on human Ch2 (1-3). Each IL-1 family member contains an IL-1 fold. This fold is generated by 12 packed beta -sheets that interact to form a beta -trefoil structure. Little amino acid (aa) homology is required to achieve this structure, and this explains the low aa identity among IL-1 family members. IL-1ra is a pure cytokine receptor antagonist that has no signal transduction-initiating activity (4). It is an acute phase protein that exists to dampen inflammation. IL-1( beta ) is initially produced by monocytes in response to a variety of stimuli. Circulating IL-1 then binds to widely expressed IL-1 type I receptors (IL-1 RI) and initiates a number of pro-inflammatory events. On endothelial cells (EC), IL-1 induces PGE2 and IL-6 release, generating fever, thrombocytosis, and hepatic acute phase protein production. In synovial joints, IL-1 induces chondrocyte NO production, an event that leads to reduced collagen synthesis and chondrocyte apoptosis. Finally, IL-1 increases neutrophil counts, both in blood and tissue, and thus is able to promote a pro-inflammatory environment in multiple locations (5-8). IL-1ra blocks IL-1 action through competitive inhibition. More correctly, although IL-1ra fills the IL-1 binding site in IL-1 RI, it is also unable to orchestrate the creation of a signal-transducing IL-1 RI:IL-1 R Accessory protein (IL-1 R AcP) heterodimer complex. Effective IL-1ra concentrations are generally 100-fold greater than local IL-1 concentrations. This is because the IL-1ra half-life is but 6 minutes, and very few IL-1 type I receptors need to be engaged by IL-1 to elicit a cellular response (5, 7, 9). 

Human IL-1ra is synthesized as a 177 aa precursor that contains a 25 aa signal sequence and a 152 aa mature region (10, 11). Although it contains an IL-1 cytokine fold, it apparently lacks two structural motifs that allow for activation of the IL-1 receptor heterodimer. First, and following binding to IL-1 RI, the presence of Ile 51-His 54 and Lys 145 of the mature molecule preclude recruitment of IL-1 R AcP. Second, there is no identifiable C-terminal lectin segment that is hypothesized to help recruit an accessory signaling component (1, 12, 13). Mature human IL-1ra is 77% and 82% aa identical to mouse and canine IL-1ra, respectively, and human IL-1ra inhibits IL-1 activity on mouse cells (10). A number of cell types express IL-1ra, including monocytes (11), Sertoli cells (14), hepatocytes (15), adipocytes (16), synovial fibroblasts (17), mast cells (18), pancreatic beta -cells (19), and intestinal epithelial cells (20). There are at least three intracellular IL-1ra isoforms (icIL-1ra1, 2, and 3). All show N-terminal variation, and all contain amino acids 35-177 of the secreted precursor (21-23). Intracellular IL-1ra1 is of particular interest, because it is reported to be "secreted" by endothelial cells and binds to the IL-1 RI in an antagonist fashion (23-25). Intracellular IL-1ra1 is 159 aa in length and shows a 3 aa substitution for the first 21 aa's of the signal sequence of IL-1ra (21).

Publications for IL-1ra/IL-1F3/IL1RN (DY280)(37)

Publications using DY280

• El-Baz, N;Kyser, A;Mahmoud, MY;Farrell, CZ;Ginocchio, S;Frieboes, HB;Doster, RS; Modulation of group B Streptococcus infection and vaginal cell inflammatory signaling in vitro by Lactobacillus crispatus-loaded electrospun fibers Infection and immunity 2025-08-27 [PMID: 40863109] (Human)
• Ghaffarpour, S;Ghazanfari, T;Ardestani, SK;Naghizadeh, MM;Vaez Mahdavi, MR;Salehi, M;Majd, AMM;Rashidi, A;Chenary, MR;Mostafazadeh, A;Rezaei, A;Khodadadi, A;Iranparast, S;Khazaei, HA; Cytokine profiles dynamics in COVID-19 patients: a longitudinal analysis of disease severity and outcomes Scientific reports 2025-04-23 [PMID: 40269030] (Human)
• Pourfarzam, S;Ardestani, SK;Jamali, T;Ghazanfari, H;Naghizadeh, MM;Faghihzadeh, S;Yaraee, R;Ghazanfari, Z;Ghazanfari, T; Distinct inflammatory profiles in mustard lung: A study of sulfur mustard-exposed patients with serious pulmonary complications International immunopharmacology 2024-12-16 [PMID: 39689604] (Human)
• Snuggs, JW;Senter, RK;Whitt, JP;Jackson, JD;Le Maitre, CL; PCRX-201, a novel IL-1Ra gene therapy treatment approach for low back pain resulting from intervertebral disc degeneration Gene therapy 2024-11-21 [PMID: 39572769] (Human)
• Duijzer, R;Dalloyaux, D;Boerrigter, MM;Lemmers, H;Dijkstra, H;van Emst, L;Te Morsche, RHM;Jaeger, M;Joosten, LAB;Drenth, JPH; Exploring the innate immune response in polycystic liver disease Cytokine 2024-12-01 [PMID: 39541862] (Human)
• Flores-Gomez, D;Hobo, W;van Ens, D;Kessler, EL;Novakovic, B;Schaap, NPM;Rijnen, WHC;Joosten, LAB;Netea, MG;Riksen, NP;Bekkering, S; Interleukin-1? induces trained innate immunity in human hematopoietic progenitor cells in vitro Stem cell reports 2024-09-25 [PMID: 39515317] (Human)
• Cab?u, G;Badii, M;Mirea, AM;Gaal, OI;van Emst, L;Popp, RA;Cri?an, TO;Joosten, LAB; Long-Lasting Enhanced Cytokine Responses Following SARS-CoV-2 BNT162b2 mRNA Vaccination Vaccines 2024-07-03 [PMID: 39066374] (Human)
• Dos Santos, JC;Moreno, M;Teufel, LU;Chilibroste, S;Keating, ST;Groh, L;Domínguez-Andrés, J;Williams, DL;Ma, Z;Lowman, DW;Ensley, HE;Novakovic, B;Ribeiro-Dias, F;Netea, MG;Chabalgoity, JA;Joosten, LAB; Leishmania braziliensis enhances monocyte responses to promote anti-tumor activity Cell reports 2024-03-07 [PMID: 38457336] (Human)
• Gysan, MR;Milacek, C;Bal, C;Zech, A;Brugger, J;Milos, RI;Antoniewicz, L;Idzko, M;Gompelmann, D; Ventilatory support and inflammatory peptides in hospitalised patients with COVID-19: A prospective cohort trial PloS one 2023-11-02 [PMID: 37917760] (Human)
• Jayaprakash Demirel, K;Wu, R;Neves Guimaraes, A;Demirel, I; The role of NLRP3 in regulating gingival epithelial cell responses evoked by Aggregatibacter actinomycetemcomitans Cytokine 2023-09-01 [PMID: 37541072] (Human)
• Scherr, BF;Reiner, MF;Baumann, F;Höhne, K;Müller, T;Ayata, K;Müller-Quernheim, J;Idzko, M;Zissel, G; Prevention of M2 polarization and temporal limitation of differentiation in monocytes by extracellular ATP BMC immunology 2023-06-23 [PMID: 37353774] (Human)
• Ferreira, AV;Kostidis, S;Groh, LA;Koeken, VACM;Bruno, M;Baydemir, I;Kilic, G;Bulut, Ö;Andriopoulou, T;Spanou, V;Synodinou, KD;Gkavogianni, T;Moorlag, SJCFM;Charlotte de Bree, L;Mourits, VP;Matzaraki, V;Koopman, WJH;van de Veerdonk, FL;Renieris, G;Giera, M;Giamarellos-Bourboulis, EJ;Novakovic, B;Domínguez-Andrés, J; Dimethyl itaconate induces long-term innate immune responses and confers protection against infection Cell reports 2023-06-16 [PMID: 37330914] ( Human)
• V Klück, G Cab?u, L Mies, F Bukkems, L van Emst, R Bakker, A van Caam, HINT conso, TO Cri?an, LAB Joosten TGF-beta is elevated in hyperuricemic individuals and mediates urate-induced hyperinflammatory phenotype in human mononuclear cells Arthritis Research & Therapy, 2023-02-27;25(1):30. 2023-02-27 [PMID: 36850003] (Human)
• LU Teufel, CI van der Ma, V Klück, A Simons, A Hoischen, V Vernimmen, LAB Joosten, RJW Arts Effect of exogenous IL-37 on immune cells from a patient carrying a potential IL37 loss-of-function variant: A case study Cytokine, 2022-12-05;162(0):156102. 2022-12-05 [PMID: 36476991] (Human)
• FE Rossi, AJ Maldonado, JM Cholewa, SLG Ribeiro, CA de Araújo, C Figueiredo, T Reichel, K Krüger, FS Lira, LG Minuzzi Exercise training-induced changes in immunometabolic markers in youth badminton athletes Scientific Reports, 2022-09-15;12(1):15539. 2022-09-15 [PMID: 36109571] (Human)
• C Aussel, E Busson, H Vantomme, J Peltzer, C Martinaud Quality assessment of a serum and xenofree medium for the expansion of human GMP-grade mesenchymal stromal cells PeerJ, 2022-05-30;10(0):e13391. 2022-05-30 [PMID: 35663525] (Human)
• J Villar, R Herrán-Mon, E González-H, M Prieto-Gon, A Ambrós, A Rodríguez-, A Muriel-Bom, R Solano, C Cuenca-Rub, A Vidal, C Flores, JM González-M, MI García-Lao, Genetics o Clinical and biological markers for predicting ARDS and outcome in septic patients Scientific Reports, 2021-11-22;11(1):22702. 2021-11-22 [PMID: 34811434] (Human)
• SC Lean, RL Jones, SA Roberts, AEP Heazell A prospective cohort study providing insights for markers of adverse pregnancy outcome in older mothers BMC pregnancy and childbirth, 2021-10-20;21(1):706. 2021-10-20 [PMID: 34670515] (Human)
• MAR Brandao-Ra, R Moraes-Fer, MC Oliveira-J, A Santos-Dia, ALL Bachi, G Gabriela-P, S de Oliveir, AC Araújo-Ros, LVF Oliveira, CR Frison, WL do Prado, RP Raju, PB Balagopal, RP Vieira Pulmonary function changes in older adults with and without metabolic syndrome Scientific Reports, 2021-08-30;11(1):17337. 2021-08-30 [PMID: 34462482] (Human)
• R Björkenhei, E Jämsen, E Eriksson, P Uppstu, L Aalto-Setä, L Hupa, KK Eklund, M Ainola, NC Lindfors, J Pajarinen Sintered S53P4 bioactive glass scaffolds have anti-inflammatory properties and stimulate osteogenesis in vitro European cells & materials, 2021-01-03;41(0):15-30. 2021-01-03 [PMID: 33389745] (Human)
• I Patlan, MP Jeffrey, D Lewis, J Green-John, S Dogra Salivary IL-8 and IL-1RA changes induced by exercise in cold-dry and normal conditions Cytokine, 2020-10-28;137(0):155347. 2020-10-28 [PMID: 33128925] (Human)
• GJ Britton, A Chen-Liaw, F Cossarini, AE Livanos, MP Spindler, T Plitt, J Eggers, I Mogno, A Gonzalez-R, S Siu, M Tankelevic, L Grinspan, RE Dixon, D Jha, G Martinez-D, F Amanat, DA Hoagland, B tenOever, MC Dubinsky, M Merad, H van Bakel, F Krammer, G Bongers, S Mehandru, JJ Faith SARS-CoV-2-specific IgA and limited inflammatory cytokines are present in the stool of select patients with acute COVID-19 medRxiv : the preprint server for health sciences, 2020-12-09;0(0):. 2020-12-09 [PMID: 32909002] (Human)
• E Kuca-Warna, U Skalska, I Janicka, U Musia?owic, K Bonek, P G?uszko, P Szcz?sny, M Olesi?ska, E Kontny The Phenotype and Secretory Activity of Adipose-Derived Mesenchymal Stem Cells (ASCs) of Patients with Rheumatic Diseases Cells, 2019-12-17;8(12):. 2019-12-17 [PMID: 31861245] (Human)
• NE Powers, B Swartzwelt, C Marchetti, DM de Graaf, A Lerchner, M Schlapschy, R Datar, U Binder, CK Edwards, A Skerra, CA Dinarello PASylation of IL-1 Receptor antagonist (IL-1Ra) retains IL-1 blockade and extends its duration in mouse urate crystal-induced peritonitis J. Biol. Chem., 2019-12-09;0(0):. 2019-12-09 [PMID: 31819009] (Human)
• A Lindblad, K Persson, I Demirel IL-1RA is part of the inflammasome-regulated immune response in bladder epithelial cells and influences colonization of uropathogenic E. coli Cytokine, 2019-07-09;123(0):154772. 2019-07-09 [PMID: 31299415] (Human)
• T Tyagi, M Alarab, Y Leong, S Lye, O Shynlova Local oestrogen therapy modulates extracellular matrix and immune response in the vaginal tissue of post-menopausal women with severe pelvic organ prolapse J. Cell. Mol. Med., 2019-02-17;0(0):. 2019-02-17 [PMID: 30772947] (Human)
• LM Scott, AH Bryant, A Rees, B Down, RH Jones, CA Thornton Production and regulation of interleukin-1 family cytokines at the materno-fetal interface Cytokine, 2017-07-13;0(0):. 2017-07-13 [PMID: 28712670] (Human)
• Damien Dietrich Interleukin-36 potently stimulates human M2 macrophages, Langerhans cells and keratinocytes to produce pro-inflammatory cytokines Cytokine, 2016-05-31;84(0):88-98. 2016-05-31 [PMID: 27259168] (Human)
• Schupp J, Binder H, Jager B, Cillis G, Zissel G, Muller-Quernheim J, Prasse A Macrophage activation in acute exacerbation of idiopathic pulmonary fibrosis. PLoS ONE, 2015-01-15;10(1):e0116775. 2015-01-15 [PMID: 25590613] (Human)
• Greenlee-Wacker M, Rigby K, Kobayashi S, Porter A, DeLeo F, Nauseef W Phagocytosis of Staphylococcus aureus by human neutrophils prevents macrophage efferocytosis and induces programmed necrosis. J Immunol, 2014-04-11;192(10):4709-17. 2014-04-11 [PMID: 24729616] (Human)
• Haddad L, Sirois M Angiopoietin-1 upregulates de novo expression of IL-1beta and Il1-Ra, and the exclusive release of Il1-Ra from human neutrophils. PLoS ONE, 2014-02-12;9(2):e88980. 2014-02-12 [PMID: 24563688] (Human)
• Uciechowski P, Oellig E, Mariani E, Malavolta M, Mocchegiani E, Rink L Effects of human Toll-like receptor 1 polymorphisms on ageing. Immun Ageing, 2013-02-07;10(1):4. 2013-02-07 [PMID: 23391127] (Human)
• Suh HS, Choi N, Tarassishin L, Lee SC Regulation of progranulin expression in human microglia and proteolysis of progranulin by matrix metalloproteinase-12 (MMP-12). PLoS ONE, 2012-04-11;7(4):e35115. 2012-04-11 [PMID: 22509390] (Human)
• Chakravarti A, Rusu D, Flamand N, Borgeat P, Poubelle PE Reprogramming of a subpopulation of human blood neutrophils by prolonged exposure to cytokines. Lab. Invest., 2009-07-27;89(10):1084-99. 2009-07-27 [PMID: 19636293] (Human)
• Janes KA, Gaudet S, Albeck JG, Nielsen UB, Lauffenburger DA, Sorger PK The response of human epithelial cells to TNF involves an inducible autocrine cascade. Cell, 2006-03-24;124(6):1225-39. 2006-03-24 [PMID: 16564013] (Human)
• Palmer G, Trolliet S, Talabot-Ayer D, Mezin F, Magne D, Gabay C Pre-interleukin-1alpha expression reduces cell growth and increases interleukin-6 production in SaOS-2 osteosarcoma cells: Differential inhibitory effect of interleukin-1 receptor antagonist (icIL-1Ra1). Cytokine, 2005-07-21;31(2):153-60. 2005-07-21 [PMID: 15923123] (Human)
• Palmer G, Talabot-Ayer D, Szalay-Quinodoz I, Maret M, Arend WP, Gabay C Mice transgenic for intracellular interleukin-1 receptor antagonist type 1 are protected from collagen-induced arthritis. Eur. J. Immunol., 2003-02-01;33(2):434-40. 2003-02-01 [PMID: 12645941] (Human)

FAQs for IL-1ra/IL-1F3/IL1RN (DY280). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: IL1RN