Human IL-1ra/IL-1F3 DuoSet ELISA, 15 Plate

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
Biotin

Order Details

Human IL-1ra/IL-1F3 DuoSet ELISA, 15 Plate Summary

Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
IL1RN

Applications/Dilutions

Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using DY280.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-1ra/IL-1F3 DuoSet ELISA, 15 Plate

  • DIRA
  • ICIL-1ra
  • IL1F3
  • IL-1F3
  • IL1ra
  • IL-1ra
  • IL-1ra3
  • IL1RN
  • IL-1RN
  • interleukin 1 receptor antagonist
  • IRAP
  • MVCD4

Background

Interleukin-1 receptor antagonist (IL-1ra; also known as IL-1F3) is a 22-25 kDa member of the IL-1 family of cytokines. Currently, there are 11 family members (IL-1F1-F11), nine of which form an IL-1 gene cluster on human Ch2 (1-3). Each IL-1 family member contains an IL-1 fold. This fold is generated by 12 packed beta -sheets that interact to form a beta -trefoil structure. Little amino acid (aa) homology is required to achieve this structure, and this explains the low aa identity among IL-1 family members. IL-1ra is a pure cytokine receptor antagonist that has no signal transduction-initiating activity (4). It is an acute phase protein that exists to dampen inflammation. IL-1( beta ) is initially produced by monocytes in response to a variety of stimuli. Circulating IL-1 then binds to widely expressed IL-1 type I receptors (IL-1 RI) and initiates a number of pro-inflammatory events. On endothelial cells (EC), IL-1 induces PGE2 and IL-6 release, generating fever, thrombocytosis, and hepatic acute phase protein production. In synovial joints, IL-1 induces chondrocyte NO production, an event that leads to reduced collagen synthesis and chondrocyte apoptosis. Finally, IL-1 increases neutrophil counts, both in blood and tissue, and thus is able to promote a pro-inflammatory environment in multiple locations (5-8). IL-1ra blocks IL-1 action through competitive inhibition. More correctly, although IL-1ra fills the IL-1 binding site in IL-1 RI, it is also unable to orchestrate the creation of a signal-transducing IL-1 RI:IL-1 R Accessory protein (IL-1 R AcP) heterodimer complex. Effective IL-1ra concentrations are generally 100-fold greater than local IL-1 concentrations. This is because the IL-1ra half-life is but 6 minutes, and very few IL-1 type I receptors need to be engaged by IL-1 to elicit a cellular response (5, 7, 9). 
Human IL-1ra is synthesized as a 177 aa precursor that contains a 25 aa signal sequence and a 152 aa mature region (10, 11). Although it contains an IL-1 cytokine fold, it apparently lacks two structural motifs that allow for activation of the IL-1 receptor heterodimer. First, and following binding to IL-1 RI, the presence of Ile 51-His 54 and Lys 145 of the mature molecule preclude recruitment of IL-1 R AcP. Second, there is no identifiable C-terminal lectin segment that is hypothesized to help recruit an accessory signaling component (1, 12, 13). Mature human IL-1ra is 77% and 82% aa identical to mouse and canine IL-1ra, respectively, and human IL-1ra inhibits IL-1 activity on mouse cells (10). A number of cell types express IL-1ra, including monocytes (11), Sertoli cells (14), hepatocytes (15), adipocytes (16), synovial fibroblasts (17), mast cells (18), pancreatic beta -cells (19), and intestinal epithelial cells (20). There are at least three intracellular IL-1ra isoforms (icIL-1ra1, 2, and 3). All show N-terminal variation, and all contain amino acids 35-177 of the secreted precursor (21-23). Intracellular IL-1ra1 is of particular interest, because it is reported to be "secreted" by endothelial cells and binds to the IL-1 RI in an antagonist fashion (23-25). Intracellular IL-1ra1 is 159 aa in length and shows a 3 aa substitution for the first 21 aa's of the signal sequence of IL-1ra (21).

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Publications for IL-1ra/IL-1F3/IL1RN (DY280)(15)

We have publications tested in 1 confirmed species: Human.


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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using DY280 Applications Species
E Kuca-Warna, U Skalska, I Janicka, U Musia?owic, K Bonek, P G?uszko, P Szcz?sny, M Olesi?ska, E Kontny The Phenotype and Secretory Activity of Adipose-Derived Mesenchymal Stem Cells (ASCs) of Patients with Rheumatic Diseases Cells, 2019;8(12):. 2019 [PMID: 31861245] (Human) Human
NE Powers, B Swartzwelt, C Marchetti, DM de Graaf, A Lerchner, M Schlapschy, R Datar, U Binder, CK Edwards, A Skerra, CA Dinarello PASylation of IL-1 Receptor antagonist (IL-1Ra) retains IL-1 blockade and extends its duration in mouse urate crystal-induced peritonitis J. Biol. Chem., 2019;0(0):. 2019 [PMID: 31819009] (Human) Human
A Lindblad, K Persson, I Demirel IL-1RA is part of the inflammasome-regulated immune response in bladder epithelial cells and influences colonization of uropathogenic E. coli Cytokine, 2019;123(0):154772. 2019 [PMID: 31299415] (Human) Human
T Tyagi, M Alarab, Y Leong, S Lye, O Shynlova Local oestrogen therapy modulates extracellular matrix and immune response in the vaginal tissue of post-menopausal women with severe pelvic organ prolapse J. Cell. Mol. Med., 2019;0(0):. 2019 [PMID: 30772947] (Human) Human
LM Scott, AH Bryant, A Rees, B Down, RH Jones, CA Thornton Production and regulation of interleukin-1 family cytokines at the materno-fetal interface Cytokine, 2017;0(0):. 2017 [PMID: 28712670] (Human) Human
Damien Dietrich Interleukin-36 potently stimulates human M2 macrophages, Langerhans cells and keratinocytes to produce pro-inflammatory cytokines Cytokine, 2016;84(0):88-98. 2016 [PMID: 27259168] (Human) Human
Schupp J, Binder H, Jager B, Cillis G, Zissel G, Muller-Quernheim J, Prasse A Macrophage activation in acute exacerbation of idiopathic pulmonary fibrosis. PLoS ONE, 2015;10(1):e0116775. 2015 [PMID: 25590613] (Human) Human
Greenlee-Wacker M, Rigby K, Kobayashi S, Porter A, DeLeo F, Nauseef W Phagocytosis of Staphylococcus aureus by human neutrophils prevents macrophage efferocytosis and induces programmed necrosis. J Immunol, 2014;192(10):4709-17. 2014 [PMID: 24729616] (Human) Human
Haddad L, Sirois M Angiopoietin-1 upregulates de novo expression of IL-1beta and Il1-Ra, and the exclusive release of Il1-Ra from human neutrophils. PLoS ONE, 2014;9(2):e88980. 2014 [PMID: 24563688] (Human) Human
Uciechowski P, Oellig E, Mariani E, Malavolta M, Mocchegiani E, Rink L Effects of human Toll-like receptor 1 polymorphisms on ageing. Immun Ageing, 2013;10(1):4. 2013 [PMID: 23391127] (Human) Human
Show All 15 Publications.

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Product General Protocols

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FAQs for IL-1ra/IL-1F3/IL1RN (DY280). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

Biotin DY280
Biotin DY280

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Bioinformatics

Gene Symbol IL1RN