Human IGF-II/IGF2 Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human IGF-II/IGF2 Quantikine ELISA Kit Summary

• Background: The Quantikine® Human IGF-II Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human IGF-II in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human IGF-II and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IGF-II showed linear curves that were parallel to the standard curves obt...ained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human IGF-II.
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• Specificity: Natural and recombinant human IGF-II.
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IGF-II/IGF2 Quantikine ELISA Kit

• C11orf43
• chromosome 11 open reading frame 43
• FLJ22066
• FLJ44734
• GRDF
• IGF2
• IGF-2
• IGFII
• IGF-II
• insulin-like growth factor 2 (somatomedin A)
• insulin-like growth factor II
• insulin-like growth factor type 2
• MSA
• PEG2
• PP9974
• somatomedin-A

Background

Insulin-like growth factor (IGF)-I (also known as somatomedin C and somatomedin A) and IGF-II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved between the human, bovine and porcine proteins (100% identity), and exhibit cross-species activity.

The IGFL (insulin-like growth factor-like) family includes four small (~11 kDa), probably secreted family members in humans and one in mouse. This family shares A and B chain cysteine motifs with the IGF superfamily, and has an additional cysteine motif within an uncleaved region corresponding to the C peptide of the IGF family.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IGF-II/IGF2 (DG200)(8)

Publications using DG200

• Courtney, Y;Head, JP;Dani, N;Chechneva, OV;Shipley, FB;Zhang, Y;Holtzman, MJ;Sadegh, C;Libermann, TA;Lehtinen, MK; Choroid plexus apocrine secretion shapes CSF proteome during mouse brain development Nature neuroscience 2025-05-28 [PMID: 40437054] (Human)
• JJ Tan, JP Guyette, K Miki, L Xiao, G Kaur, T Wu, L Zhu, KJ Hansen, KH Ling, DJ Milan, HC Ott Human iPS-derived pre-epicardial cells direct cardiomyocyte aggregation expansion and organization in vitro Nature Communications, 2021-08-17;12(1):4997. 2021-08-17 [PMID: 34404774] (Human)
• N Requena-Oc, P Araos, M Flores, N García-Mar, D Silva-Peña, J Aranda, P Rivera, JJ Ruiz, A Serrano, FJ Pavón, J Suárez, F Rodríguez Evaluation of neurotrophic factors and education level as predictors of cognitive decline in alcohol use disorder Scientific Reports, 2021-08-02;11(1):15583. 2021-08-02 [PMID: 34341419] (Human)
• N García-Mar, N Pizarro, FJ Pavón, M Martínez-H, M Flores-Lóp, N Requena-Oc, P Araos, D Silva-Peña, J Suárez, LJ Santín, R de la Torr, F Rodríguez, A Serrano Potential association of plasma lysophosphatidic acid (LPA) species with cognitive impairment in abstinent alcohol use disorders outpatients Sci Rep, 2020-10-13;10(1):17163. 2020-10-13 [PMID: 33051508] (Human)
• T Manabe, H Yasuda, H Terai, H Kagiwada, J Hamamoto, T Ebisudani, K Kobayashi, K Masuzawa, S Ikemura, I Kawada, Y Hayashi, K Fukui, K Horimoto, K Fukunaga, K Soejima IGF2 autocrine-mediated IGF1R activation is a clinically relevant mechanism of osimertinib resistance in lung cancer Mol. Cancer Res., 2020-01-15;0(0):. 2020-01-15 [PMID: 31941753] (Human)
• A Federico, M Dallio, M Masarone, AG Gravina, R Di Sarno, C Tuccillo, V Cossiga, S Lama, P Stiuso, F Morisco, M Persico, C Loguercio Evaluation of the Effect Derived from Silybin with Vitamin D and Vitamin E Administration on Clinical, Metabolic, Endothelial Dysfunction, Oxidative Stress Parameters, and Serological Worsening Markers in Nonalcoholic Fatty Liver Disease Patients Oxid Med Cell Longev, 2019;2019(0):8742075. 2019 [PMID: 31737175] (Human)
• N Dai, F Ji, J Wright, L Minichiell, RI Sadreyev, J Avruch IGF2 mRNA binding protein-2 is a tumor promoter that drives cancer proliferation through its client mRNAs IGF2 and HMGA1 Elife, 2017-07-28;6(0):. 2017-07-28 [PMID: 28753127] (Human)
• Howard H Bailey Phase IIa, randomized placebo-controlled trial of single high dose cholecalciferol (vitamin D3) and daily Genistein (G-2535) versus double placebo in men with early stage prostate cancer undergoing prostatectomy Am J Clin Exp Urol, 2016-09-20;4(2):17-27. 2016-09-20 [PMID: 27766277] (Human)

FAQs for IGF-II/IGF2 (DG200). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.