Human IFN-beta Quantikine QuicKit ELISA

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human IFN-beta Quantikine QuicKit ELISA Summary

• Background:
  The Quantikine™ QuicKit™ Human IFN-beta Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human IFN-beta levels in cell culture supernates. It contains CHO cell-expressed recombinant human IFN-beta and antibodies raised against the recombinant protein. Results obtained using natural human IFN-beta showed linear curves that were parallel to the stand...ard curves obtained using the QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-beta .
  Show More
• Specificity: Natural and recombinant human IFN-beta .
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IFN-beta Quantikine QuicKit ELISA

• Fibroblast interferon
• IFB
• IFBIFNB
• IFF
• IFNB
• IFNB1
• IFNbeta
• IFN-beta
• interferon beta
• interferon, beta 1, fibroblast
• MGC96956

Background

Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.

Publications for IFN-beta (QK410)(5)

Publications using QK410

• Hasler, WA;McKay, E;Datta, G;Johnson, S;Rezagholizadeh, N;Chen, X; SARS-CoV-2 spike triggers TLR7-dependent endolysosome dysfunction and senescence in human astrocytes Journal of neuroinflammation 2025-10-31 [PMID: 41174694] (Human)
• Ren, J;Wang, S;Zong, Z;Pan, T;Liu, S;Mao, W;Huang, H;Yan, X;Yang, B;He, X;Zhou, F;Zhang, L; TRIM28-mediated nucleocapsid protein SUMOylation enhances SARS-CoV-2 virulence Nature communications 2024-01-04 [PMID: 38172120] (Mouse, Transgenic Mouse, Human)
• Ren, Y;Wang, A;Zhang, B;Ji, W;Zhu, XX;Lou, J;Huang, M;Qiu, Y;Zhou, X; Human cytomegalovirus UL36 inhibits IRF3-dependent immune signaling to counterbalance its immunoenhancement as apoptotic inhibitor Science advances 2023-10-06 [PMID: 37792941] (Human)
• M Perego, S Fu, Y Cao, A Kossenkov, M Yao, K Alicea-Tor, W Liu, Z Jiang, Z Chen, SY Fuchs, J Zhou, DI Gabrilovic Mechanisms regulating transitory suppressive activity of neutrophils in newborns: PMNs-MDSCs in newborns Oncogene, 2022-06-21;0(0):. 2022-06-21 [PMID: 35726818] (Human)
• WJ Melvin, CO Audu, FM Davis, SB Sharma, A Joshi, A DenDekker, S Wolf, E Barrett, K Mangum, X Zhou, M Bame, A Ruan, A Obi, SL Kunkel, BB Moore, KA Gallagher Coronavirus induces diabetic macrophage-mediated inflammation via SETDB2 Proceedings of the National Academy of Sciences of the United States of America, 2021-09-21;118(38):. 2021-09-21 [PMID: 34479991] (Human)

FAQs for IFN-beta (QK410). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.