Human Flt-3 Ligand/FLT3L DuoSet ELISA, 15 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human Flt-3 Ligand/FLT3L DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: FLT3LG

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Flt-3 Ligand/FLT3L DuoSet ELISA, 15 Plate

• FL
• FLG3L
• Flt3 ligand
• Flt-3 Ligand
• Flt3L
• FLT3LG
• fms-related tyrosine kinase 3 ligand
• SL cytokine

Background

Flt-3 Ligand, also known as FL, is an alpha-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. Mature human Flt-3 Ligand consists of a 158 amino acid (aa) extracellular domain (ECD) with a cytokine-like domain and a juxtamembrane tether region, a 21 aa transmembrane segment, and a 30 aa cytoplasmic tail. Within the ECD, human Flt-3 Ligand shares 71% and 65% aa sequence identity with mouse and rat Flt-3 Ligand, respectively. Human and mouse Flt-3 Ligand show cross-species activity. Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts. Each 36 kDa chain carries approximately 12 kDa of N- and O-linked carbohydrates. Alternate splicing and proteolytic cleavage of the transmembrane form can generate a soluble 30 kDa fragment that includes the cytokine domain. Alternate splicing of human Flt-3 Ligand also generates membrane-associated isoforms that contain either a truncated cytoplasmic tail or an 85 aa substitution following the cytokine domain. Both transmembrane and soluble Flt-3 Ligand signal through the tyrosine kinase receptor Flt-3/Flk-2. Flt-3 Ligand induces the expansion of monocytes and immature dendritic cells as well as early B cell lineage differentiation. It synergizes with IL-3, GM-CSF, and SCF to promote the mobilization and myeloid differentiation of hematopoietic stem cells. It cooperates with IL-2, -6, -7, and -15 to induce NK cell development and with IL-3, -7, and -11 to induce terminal B cell maturation. Animal studies also show Flt-3 Ligand to reduce the severity of experimentally induced allergic inflammation.

Publications for Flt-3 Ligand/FLT3L (DY308)(5)

Publications using DY308

• P Peterlin, J Gaschet, T Guillaume, A Garnier, M Eveillard, A Le Bourgeo, M Cherel, C Debord, Y Le Bris, O Theisen, C Godon, B Mahé, V Dubruille, S Wuilleme, C Touzeau, T Gastinne, N Blin, A Lok, B Tessoulin, S Le Gouill, P Moreau, MC Béné, P Chevallier A new cytokine-based dynamic stratification during induction is highly predictive of survivals in acute myeloid leukemia Cancer Medicine, 2020-12-25;0(0):. 2020-12-25 [PMID: 33369136] (Human)
• P Peterlin, J Gaschet, T Guillaume, A Garnier, M Eveillard, A Le Bourgeo, M Cherel, C Debord, Y Le Bris, O Theisen, B Mahé, V Dubruille, C Godon, N Robillard, S Wuilleme, C Touzeau, T Gastinne, N Blin, A Lok, A Bonnet, S Le Gouill, P Moreau, MC Béné, P Chevallier FLT3 ligand plasma levels have no impact on outcomes after allotransplant in acute leukemia Cytokine, 2019-04-28;120(0):85-87. 2019-04-28 [PMID: 31035172] (Human)
• P Peterlin, J Gaschet, T Guillaume, A Garnier, M Eveillard, A Le Bourgeo, M Cherel, C Debord, Y Le Bris, O Theisen, B Mahé, V Dubruille, C Godon, N Robillard, S Wuillem, C Touzeau, T Gastinne, N Blin, A Lok, A Bonnet, S Le Gouill, P Moreau, MC Béné, P Chevallier FLT3 ligand plasma levels in acute myeloid leukemia Haematologica, 2019-01-10;0(0):. 2019-01-10 [PMID: 30630980] (Human)
• Andersson S, Svensson M, Erlandsson M, Dehlin M, Andersson K, Bokarewa M Activation of Fms-like tyrosine kinase 3 signaling enhances survivin expression in a mouse model of rheumatoid arthritis. PLoS ONE, 2012-10-17;7(10):e47668. 2012-10-17 [PMID: 23082191] (Human)
• Erlendsson LS, Muench MO, Hellman U, Hrafnkelsdottir SM, Jonsson A, Balmer Y, Mantyla E, Orvar BL Barley as a green factory for the production of functional Flt3 ligand. Biotechnol J, 2010-02-01;5(2):163-71. 2010-02-01 [PMID: 19844912] (Human)

Bioinformatics

• Gene Symbol: FLT3LG