Human Fas/TNFRSF6/CD95 Quantikine ELISA Kit

Product Discontinued

Human Fas/TNFRSF6/CD95 Quantikine ELISA Kit Summary

• Background: The Quantikine Human sFas Immunoassay is a 4.5 hour solid phase ELISA designed to measure human sFas in cell culture supernates, serum, and plasma. It contains recombinant human Fas/Fc chimera expressed from NS0 cells and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained measuring natural human ...sFas showed dose-response curves that were parallel to the standard curves obtained using the recombinant Fas/Fc chimera. These results indicate that this kit can be used to determine relative mass values for natural human sFas.
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• Specificity: Natural and recombinant human sFas
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: FAS

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Fas/TNFRSF6/CD95 Quantikine ELISA Kit

• Apo-1 antigen
• Apo-1
• apoptosis antigen 1
• Apoptosis-mediating surface antigen FAS
• APT1
• APT1FASTM
• CD95 antigen
• CD95
• CD95ALPS1A
• Fas (TNF receptor superfamily, member 6)
• Fas AMA
• Fas antigen
• Fas
• FAS1
• FASLG receptor
• TNFRSF6
• TNFRSF6member 6
• tumor necrosis factor receptor superfamily member 6

Background

Fas (Fibroblast-associated), also known as Apo-1, CD95, and TNFRSF6, was originally identified as a cell-surface protein that binds to monoclonal antibodies that are cytolytic for various human cell lines. Alternatively spliced cDNAs encoding multiple Fas isoforms, including a soluble form of Fas, have been identified. Fas is highly expressed in epithelial cells, hepatocyes, activated mature lymphocytes, virus-transformed lymphocytes and other tumor cells. Fas expression has also been detected in mouse thymus, liver, heart, lung, kidney and ovary.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Fas/TNFRSF6/CD95 (DFS00)(17)

Publications using DFS00

• R Grzanka, A Damasiewic, A Kasperska- Tumor necrosis factor-alpha and Fas/Fas ligand signaling pathways in chronic spontaneous urticaria Allergy Asthma Clin Immunol, 2019-03-14;15(0):15. 2019-03-14 [PMID: 30911316] (Human)
• A Karowicz-B, U Kowalska-K, D Estemberg, A Sikora-Szu Evaluation of soluble concentration Fas and Fas ligand in maternal and cord blood 3rd trimester of pregnancy Ginekol. Pol., 2018-01-01;89(3):142-146. 2018-01-01 [PMID: 29664549] (Human)
• Sehgal , L, Mathur , R, Braun , F K, Wise , J F, Berkova , Z, Neelapu , S, Kwak , L W, Samaniego , F FAS-antisense 1 lncRNA and production of soluble versus membrane Fas in B-cell lymphoma. Leukemia, 2014-04-03;28(12):2376-87. 2014-04-03 [PMID: 24811343] (Human)
• Chang J, Zhang G, Zhang L, Hou Y, Liu X, Zhang L High admission glucose levels increase Fas apoptosis and mortality in patients with acute ST-elevation myocardial infarction: a prospective cohort study. Cardiovasc Diabetol, 2013-11-15;12(1):171. 2013-11-15 [PMID: 24229414] (Human)
• Deftereos S, Giannopoulos G, Kossyvakis C, Kaoukis A, Raisakis K, Driva M, Panagopoulou V, Lappos S, Rentoukas I, Pyrgakis V, Alpert MA Effect of quinapril on in-stent restenosis and relation to plasma apoptosis signaling molecules. Am. J. Cardiol., 2009-11-14;105(1):54-8. 2009-11-14 [PMID: 20102890] (Human)
• Zhao WS, Xu L, Wang LF, Zhang L, Zhang ZY, Liu Y, Liu XL, Yang XC, Cui L, Zhang L A 60-s postconditioning protocol by percutaneous coronary intervention inhibits myocardial apoptosis in patients with acute myocardial infarction. Apoptosis, 2009-10-01;14(10):1204-11. 2009-10-01 [PMID: 19693675] (Human)
• Fas expression in conjunctival epithelial cells of patients with cystic fibrosis. J. Interferon Cytokine Res., 2009-11-01;29(11):735-40. 2009-11-01 [PMID: 19642903] (Human)
• Niessner A, Hohensinner PJ, Rychli K, Neuhold S, Zorn G, Richter B, Hulsmann M, Berger R, Mortl D, Huber K, Wojta J, Pacher R Prognostic value of apoptosis markers in advanced heart failure patients. Eur. Heart J., 2009-02-04;30(7):789-96. 2009-02-04 [PMID: 19196721] (Human)
• Perez-Andres M, Almeida J, Martin-Ayuso M, De Las Heras N, Moro MJ, Martin-Nunez G, Galende J, Cuello R, Abuin I, Moreno I, Dominguez M, Hernandez J, Mateo G, San Miguel JF, Orfao A Soluble and membrane levels of molecules involved in the interaction between clonal plasma cells and the immunological microenvironment in multiple myeloma and their association with the characteristics of the disease. Int. J. Cancer, 2009-01-15;124(2):367-75. 2009-01-15 [PMID: 19003959] (Human)
• Kourea K, Parissis JT, Farmakis D, Panou F, Paraskevaidis I, Venetsanou K, Filippatos G, Kremastinos DT Effects of darbepoetin-alpha on plasma pro-inflammatory cytokines, anti-inflammatory cytokine interleukin-10 and soluble Fas/Fas ligand system in anemic patients with chronic heart failure. Atherosclerosis, 2007-11-07;199(1):215-21. 2007-11-07 [PMID: 17991471] (Human)
• Svatek RS, Herman MP, Lotan Y, Casella R, Hsieh JT, Sagalowsky AI, Shariat SF Soluble Fas--a promising novel urinary marker for the detection of recurrent superficial bladder cancer. Cancer, 2006-04-15;106(8):1701-7. 2006-04-15 [PMID: 16541433] (Human)
• Parissis JT, Adamopoulos S, Karatzas D, Paraskevaidis J, Livanis E, Kremastinos D Growth hormone-induced reduction of soluble apoptosis mediators is associated with reverse cardiac remodelling and improvement of exercise capacity in patients with idiopathic dilated cardiomyopathy. Eur J Cardiovasc Prev Rehabil, 2005-04-01;12(2):164-8. 2005-04-01 [PMID: 15785303] (Human)
• Zhang L, Li YH, Zhang HY, Chen ML, Gao MM, Hu AH, Yang HS High-dose glucose-insulin-potassium treatment reduces myocardial apoptosis in patients with acute myocardial infarction. Eur. J. Clin. Invest., 2005-03-01;35(3):164-70. 2005-03-01 [PMID: 15733070] (Human)
• Alter P, Maisch B Escape from cardiomyocyte apoptosis by enterovirus persistence due to elevated soluble Fas-receptors. Z Kardiol, 2004-07-01;93(7):524-32. 2004-07-01 [PMID: 15243763] (Human)
• Lee TH, Wu MY, Chen MJ, Chao KH, Ho HN, Yang YS Nitric oxide is associated with poor embryo quality and pregnancy outcome in in vitro fertilization cycles. Fertil. Steril., 2004-07-01;82(1):126-31. 2004-07-01 [PMID: 15237000] (Human)
• Mahovic D, Petravic D, Petelin Z, Zurak N, Horvat G, Hajnsek S Level of sFas/APO 1 in serum and cerebrospinal fluid in multiple sclerosis. Clin Neurol Neurosurg, 2004-06-01;106(3):230-2. 2004-06-01 [PMID: 15177773] (Human)
• Dziankowska-Bartkowiak B, Waszczykowska E, Zalewska A, Sysa-Jedrzejowska A Evaluation of caspase 1 and sFas serum levels in patients with systemic sclerosis: correlation with lung dysfunction, joint and bone involvement. Mediators Inflamm., 2003-12-01;12(6):339-43. 2003-12-01 [PMID: 14668093] (Human)

FAQs for Fas/TNFRSF6/CD95 (DFS00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: FAS