Human CXCL1/GRO alpha Quantikine ELISA Kit, 2nd Gen

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human CXCL1/GRO alpha Quantikine ELISA Kit, 2nd Gen Summary

• Background: The Quantikine^® Human CXCL1/GRO alpha Immunoassay is a 4.25-4.5 hour solid phase ELISA designed to measure human GRO alpha in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human GRO alpha and antibodies raised against the recombinant protein. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtaine...d using natural human GRO alpha showed linear curves that were parallel to the
  standard curves obtained using the Quantikine^® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human GRO alpha .
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• Specificity: Natural and recombinant human GRO alpha .
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CXCL1/GRO alpha Quantikine ELISA Kit, 2nd Gen

• CINC1
• CINC-1
• CXCL1
• FSP
• GRO alpha
• GRO1
• GROa
• KC
• MGSA
• MGSA-a
• MGSA-alpha
• NAP-3
• SCYB1

Background

CXCL1/GRO alpha, also known as KC in mouse and CINC-1 in rat, is a member of the CXC family of chemokines. Human CXCL1/GRO alpha is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 57% and 59% amino acid sequence identity with the human protein, respectively. Genome-wide analysis suggests that the protein encoded by the mouse Cxcl3 gene is homologous to human CXCL1/GRO alpha protein. Human CXCL1/GRO alpha can be cleaved into three isoforms CXCL1/GRO alpha (aa 4-73), CXCL1/GRO alpha (aa 5-73), and CXCL1/GRO alpha (aa 6-73) which show higher activity than the full length protein. CXCL1/GRO alpha is secreted by mast cells and macrophages whereupon it acts as a chemoattractant for neutrophils.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CXCL1/GRO alpha/KC/CINC-1 (DGR00B)(57)

Publications using DGR00B

• Tung, JP;Simonova, G;Hewlett, E;Pedersen, SE;Lallen, AE;Alkhureeb, F;Wellburn, R;Chiaretti, S;Temple, FT;Duong, T;Cinel, M;Giles, C;Meikle, PJ;Fraser, JF; Pre-storage leucoreduction of platelet concentrates reduced biological response modifier concentrations and TRALI incidence in an in vivo sheep model Scientific reports 2025-11-26 [PMID: 41298662] (Human)
• Park, SE;Kwon, SJ;Kim, SJ;Jeong, JB;Kim, MJ;Choi, SJ;Oh, SY;Ryu, GH;Jeon, HB;Chang, JW; Anti-necroptotic effects of human Wharton's jelly-derived mesenchymal stem cells in skeletal muscle cell death model via secretion of GRO-? PloS one 2024-12-02 [PMID: 39621655] (Human)
• Di, Y;Wang, Z;Xiao, J;Zhang, X;Ye, L;Wen, X;Qin, J;Lu, L;Wang, X;He, W; ACSL6-activated IL-18R1-NF-?B promotes IL-18-mediated tumor immune evasion and tumor progression Science advances 2024-09-20 [PMID: 39292786] (Xenograft)
• Matsui, C;Koide, H;Ikeda, T;Ikegami, T;Yamamoto, T;Escandón, JM;Mohammad, A;Ito, T;Mizuno, H; Cytokines released from human adipose tissue-derived stem cells by bFGF stimulation: Effects of IL-8 and CXCL-1 on wound healing Regenerative therapy 2024-06-01 [PMID: 39045577] (Human)
• Dai, Y;Xu, J;Gong, X;Wei, J;Gao, Y;Chai, R;Lu, C;Zhao, B;Kang, Y; Human Fallopian Tube-Derived Organoids with TP53 and RAD51D Mutations Recapitulate an Early Stage High-Grade Serous Ovarian Cancer Phenotype In Vitro International journal of molecular sciences 2024-01-10 [PMID: 38255960] (Human)
• Guo, C;Sharp, A;Gurel, B;Crespo, M;Figueiredo, I;Jain, S;Vogl, U;Rekowski, J;Rouhifard, M;Gallagher, L;Yuan, W;Carreira, S;Chandran, K;Paschalis, A;Colombo, I;Stathis, A;Bertan, C;Seed, G;Goodall, J;Raynaud, F;Ruddle, R;Swales, KE;Malia, J;Bogdan, D;Tiu, C;Caldwell, R;Aversa, C;Ferreira, A;Neeb, A;Tunariu, N;Westaby, D;Carmichael, J;Fenor de la Maza, MLD;Yap, C;Matthews, R;Badham, H;Prout, T;Turner, A;Parmar, M;Tovey, H;Riisnaes, R;Flohr, P;Gil, J;Waugh, D;Decordova, S;Schlag, A;Calì, B;Alimonti, A;de Bono, JS; Targeting myeloid chemotaxis to reverse prostate cancer therapy resistance Nature 2023-10-16 [PMID: 37844613] (Human)
• Kim, HJ;Chang, HK;Lee, YM;Heo, K; Catecholamines Promote Ovarian Cancer Progression through Secretion of CXC-Chemokines International journal of molecular sciences 2023-09-14 [PMID: 37762405] (Human)
• Zhou, J;Xu, W;Wu, Y;Wang, M;Zhang, N;Wang, L;Feng, Y;Zhang, T;Wang, L;Mao, A; GPR37 promotes colorectal cancer liver metastases by enhancing the glycolysis and histone lactylation via Hippo pathway Oncogene 2023-09-25 [PMID: 37749229] (Human)
• Łukaszewicz-Zając, M;Zajkowska, M;Pączek, S;Kulczyńska-Przybik, A;Safiejko, K;Juchimiuk, M;Kozłowski, L;Mroczko, B; The Significance of CXCL1 and CXCR1 as Potential Biomarkers of Colorectal Cancer Biomedicines 2023-07-07 [PMID: 37509572] (Human)
• Gr�ttner, J;van Rijn, JM;Geiser, P;Florbrant, A;Webb, DL;Hellstr�m, PM;Sundbom, M;Sellin, ME;Sv�rd, SG; Trophozoite fitness dictates the intestinal epithelial cell response to Giardia intestinalis infection PLoS pathogens 2023-05-01 [PMID: 37141303] (Human)
• YC Tsai, TY Wang, CL Hsu, WC Lin, JY Chen, JH Li, YS Pu, AL Cheng, JC Cheng, SF Su Selective inhibition of HDAC6 promotes bladder cancer radiosensitization and mitigates the radiation-induced CXCL1 signalling British Journal of Cancer, 2023-02-21;0(0):. 2023-02-21 [PMID: 36810912] (Human)
• J Kami?ska, M Tylicka, V Dymicka-Pi, Z Mariak, J Matowicka-, OM Koper-Lenk Canonical NF-kappaB signaling pathway and GRO-alpha/CXCR2 axis are activated in unruptured intracranial aneurysm patients Scientific Reports, 2022-12-09;12(1):21375. 2022-12-09 [PMID: 36494512] (Human)
• R Dinami, L Pompili, E Petti, M Porru, C D'Angelo, S Di Vito, A Rizzo, V Campani, G De Rosa, A Bruna, V Serra, M Mano, M Giacca, C Leonetti, G Ciliberto, M Tarsounas, A Stoppaccia, S Schoeftner, A Biroccio MiR-182-3p targets TRF2 and impairs tumor growth of triple-negative breast cancer Embo Molecular Medicine, 2022-11-25;0(0):e16033. 2022-11-25 [PMID: 36426578] (Human)
• AFP Santos, LCC Cervantes, L Panahipour, FÁ Souza, R Gruber Proof-of-Principle Study Suggesting Potential Anti-Inflammatory Activity of Butyrate and Propionate in Periodontal Cells International Journal of Molecular Sciences, 2022-09-20;23(19):. 2022-09-20 [PMID: 36232340] (Human)
• T Takikawa, S Hamada, R Matsumoto, Y Tanaka, F Kataoka, A Sasaki, A Masamune Senescent Human Pancreatic Stellate Cells Secrete CXCR2 Agonist CXCLs to Promote Proliferation and Migration of Human Pancreatic Cancer AsPC-1 and MIAPaCa-2 Cell Lines International Journal of Molecular Sciences, 2022-08-17;23(16):. 2022-08-17 [PMID: 36012531] (Human)
• S Wang, J Bai, YL Zhang, QY Lin, X Han, WK Qu, PF Zhang, YS Ge, Q Zhao, HH Li CXCL1-CXCR2 signalling mediates hypertensive retinopathy by inducing macrophage infiltration Redox Biology, 2022-08-13;56(0):102438. 2022-08-13 [PMID: 35981418] (Human)
• N Lin, L Chen, Y Zhang, Y Yang, L Zhang, L Chen, P Zhang, H Su, M Yin KIF4A promotes tumor progression of bladder cancer via CXCL5 dependent myeloid-derived suppressor cells recruitment Scientific Reports, 2022-04-10;12(1):6015. 2022-04-10 [PMID: 35399116] (Human)
• N Komabashir, F Suehiro, M Ishii, M Nishimura Efficacy of chitinase-3-like protein 1 as an in�vivo bone formation predictable marker of maxillary/mandibular bone marrow stromal cells Regenerative Therapy, 2021-03-27;18(0):38-50. 2021-03-27 [PMID: 33869686] (Human)
• YY Wang, HD Chen, S Lo, YK Chen, YC Huang, SC Hu, YC Hsieh, AC Hung, MF Hou, SF Yuan Visfatin Enhances Breast Cancer Progression through CXCL1 Induction in Tumor-Associated Macrophages Cancers, 2020-11-26;12(12):. 2020-11-26 [PMID: 33256011] (Human)
• M Miyake, H Furuya, S Onishi, K Hokutan, S Anai, O Chan, S Shi, K Fujimoto, S Goodison, W Cai, CJ Rosser Monoclonal Antibody against CXCL1 (HL2401) as a Novel Agent in Suppressing IL6 Expression and Tumoral Growth Theranostics, 2019-01-25;9(3):853-867. 2019-01-25 [PMID: 30809313] (Human)
• S Grygorczuk, R ?wierzbi?s, M Kondrusik, J Dunaj, P Czupryna, A Moniuszko, A Siemieniak, S Pancewicz The intrathecal expression and pathogenetic role of Th17 cytokines and CXCR2-binding chemokines in tick-borne encephalitis J Neuroinflammation, 2018-04-20;15(1):115. 2018-04-20 [PMID: 29678185] (Human)
• DW Edwardson, J Boudreau, J Mapletoft, C Lanner, AT Kovala, AM Parissenti Inflammatory cytokine production in tumor cells upon chemotherapy drug exposure or upon selection for drug resistance PLoS ONE, 2017-09-15;12(9):e0183662. 2017-09-15 [PMID: 28915246] (Human)
• H Zhang, J Yue, Z Jiang, R Zhou, R Xie, Y Xu, S Wu CAF-secreted CXCL1 conferred radioresistance by regulating DNA damage response in a ROS-dependent manner in esophageal squamous cell carcinoma Cell Death Dis, 2017-05-18;8(5):e2790. 2017-05-18 [PMID: 28518141] (Human)
• D Wang, H Sun, J Wei, B Cen, RN DuBois CXCL1 is critical for pre-metastatic niche formation and metastasis in colorectal cancer Cancer Res., 2017-04-28;0(0):. 2017-04-28 [PMID: 28455419] (Mouse)
• MicroRNAs regulate cytokine responses in gingival epithelial cells Infect Immun, 2016-11-18;0(0):. 2016-11-18 [PMID: 27600506] (Human)
• Zhang W, Zheng X, DU L, Sun J, Shen Z, Shi C, Sun S, Zhang Z, Chen X, Qin M, Liu X, Tao J, Jia L, Fan H, Zhou B, Yu Y, Ying H, Hui L, Liu X, Yi X, Liu X, Zhang L, Duan S High salt primes a specific activation state of macrophages, M(Na). Cell Res, 2015-07-24;25(8):893-910. 2015-07-24 [PMID: 26206316] (Human)
• Rudisch A, Dewhurst M, Horga L, Kramer N, Harrer N, Dong M, van der Kuip H, Wernitznig A, Bernthaler A, Dolznig H, Sommergruber W High EMT Signature Score of Invasive Non-Small Cell Lung Cancer (NSCLC) Cells Correlates with NFkappaB Driven Colony-Stimulating Factor 2 (CSF2/GM-CSF) Secretion by Neighboring Stromal Fibroblasts. PLoS ONE, 2015-04-28;10(4):e0124283. 2015-04-28 [PMID: 25919140] (Human)
• Kawada M, Inoue H, Ohba S, Yoshida J, Masuda T, Yamasaki M, Usami I, Sakamoto S, Abe H, Watanabe T, Yamori T, Shibasaki M, Nomoto A Stromal cells positively and negatively modulate the growth of cancer cells: stimulation via the PGE2-TNFalpha-IL-6 pathway and inhibition via secreted GAPDH-E-cadherin interaction. PLoS ONE, 2015-03-18;10(3):e0119415. 2015-03-18 [PMID: 25785838] (Human)
• Lowe J, Menendez D, Bushel P, Shatz M, Kirk E, Troester M, Garantziotis S, Fessler M, Resnick M p53 and NF-kappaB coregulate proinflammatory gene responses in human macrophages. Cancer Res, 2014-04-15;74(8):2182-92. 2014-04-15 [PMID: 24737129] (Human)
• Nischalke H, Berger C, Lutz P, Langhans B, Wolter F, Eisenhardt M, Kramer B, Kokordelis P, Glassner A, Muller T, Rosendahl J, Fischer J, Berg T, Grunhage F, Leifeld L, Soyka M, Nattermann J, Sauerbruch T, Stickel F, Spengler U Influence of the CXCL1 rs4074 A allele on alcohol induced cirrhosis and HCC in patients of European descent. PLoS ONE, 2013-11-18;8(11):e80848. 2013-11-18 [PMID: 24260493] (Human)
• Hong S, Zhang X, Chen J, Zhou J, Zheng Y, Xu C Targeted gene silencing using a follicle-stimulating hormone peptide-conjugated nanoparticle system improves its specificity and efficacy in ovarian clear cell carcinoma in vitro. J Ovarian Res, 2013-11-20;6(1):80. 2013-11-20 [PMID: 24252539] (Human)
• Sapey E, Greenwood H, Walton G, Mann E, Love A, Aaronson N, Insall R, Stockley R, Lord J Phosphoinositide 3-kinase inhibition restores neutrophil accuracy in the elderly: toward targeted treatments for immunosenescence. Blood, 2013-11-04;123(2):239-48. 2013-11-04 [PMID: 24191150] (Human)
• Divella R, Daniele A, Savino E, Palma F, Bellizzi A, Giotta F, Simone G, Lioce M, Quaranta M, Paradiso A, Mazzocca A Circulating levels of transforming growth factor-betaeta (TGF-beta) and chemokine (C-X-C motif) ligand-1 (CXCL1) as predictors of distant seeding of circulating tumor cells in patients with metastatic breast cancer. Anticancer Res, 2013-04-01;33(4):1491-7. 2013-04-01 [PMID: 23564790] (Human)
• Sakitani K, Hirata Y, Hayakawa Y, Serizawa T, Nakata W, Takahashi R, Kinoshita H, Sakamoto K, Nakagawa H, Akanuma M, Yoshida H, Maeda S, Koike K Role of interleukin-32 in Helicobacter pylori-induced gastric inflammation. Infect Immun, 2012-08-13;80(11):3795-803. 2012-08-13 [PMID: 22890997] (Human)
• Yang G, Rosen DG, Liu G CXCR2 promotes ovarian cancer growth through dysregulated cell cycle, diminished apoptosis, and enhanced angiogenesis. Clin. Cancer Res., 2010-05-26;16(15):3875-86. 2010-05-26 [PMID: 20505188] (Human)
• Su Y, Amiri KI, Horton LW A phase I trial of bortezomib with temozolomide in patients with advanced melanoma: toxicities, antitumor effects, and modulation of therapeutic targets. Clin. Cancer Res., 2009-12-22;16(1):348-57. 2009-12-22 [PMID: 20028756] (Human)
• Rudack C, Sachse F, Albert N, Becker K, von Eiff C Immunomodulation of nasal epithelial cells by Staphylococcus aureus-derived serine proteases. J. Immunol., 2009-11-16;183(11):7592-601. 2009-11-16 [PMID: 19917683] (Human)
• Wong CK, Hu S, Cheung PF Thymic stromal lymphopoietin induces chemotactic and prosurvival effects in eosinophils: implications in allergic inflammation. Am. J. Respir. Cell Mol. Biol., 2009-10-20;43(3):305-15. 2009-10-20 [PMID: 19843704] (Human)
• Jonsson D, Amisten S, Bratthall G, Holm A, Nilsson BO LPS induces GROalpha chemokine production via NF-kappaB in oral fibroblasts. Inflamm. Res., 2009-05-09;58(11):791-6. 2009-05-09 [PMID: 19430878] (Human)
• Salim JP, Goette NP, Lev PR, Chazarreta CD, Heller PG, Alvarez C, Molinas FC, Marta RF Dysregulation of stromal derived factor 1/CXCR4 axis in the megakaryocytic lineage in essential thrombocythemia. Br. J. Haematol., 2008-11-01;144(1):69-77. 2008-11-01 [PMID: 19006565] (Human)
• Wright JF, Bennett F, Li B, Brooks J, Luxenberg DP, Whitters MJ, Tomkinson KN, Fitz LJ, Wolfman NM, Collins M, Dunussi-Joannopoulos K, Chatterjee-Kishore M, Carreno BM The human IL-17F/IL-17A heterodimeric cytokine signals through the IL-17RA/IL-17RC receptor complex. J. Immunol., 2008-08-15;181(4):2799-805. 2008-08-15 [PMID: 18684971] (Human)
• Skov L, Beurskens FJ, Zachariae CO, Reitamo S, Teeling J, Satijn D, Knudsen KM, Boot EP, Hudson D, Baadsgaard O, Parren PW, van de Winkel JG IL-8 as antibody therapeutic target in inflammatory diseases: reduction of clinical activity in palmoplantar pustulosis. J. Immunol., 2008-07-01;181(1):669-79. 2008-07-01 [PMID: 18566434] (Human)
• Lee SJ, Nam WD, Na HJ, Cho YL, Ha KS, Hwang JY, Lee H, Kim SO, Kwon YG, Kim YM CT20126, a novel immunosuppressant, prevents collagen-induced arthritis through the down-regulation of inflammatory gene expression by inhibiting NF-kappaB activation. Biochem. Pharmacol., 2008-04-18;76(1):79-90. 2008-04-18 [PMID: 18513703] (Human)
• Le Quement C, Guenon I, Gillon JY, Lagente V, Boichot E MMP-12 induces IL-8/CXCL8 secretion through EGFR and ERK1/2 activation in epithelial cells. Am. J. Physiol. Lung Cell Mol. Physiol., 2008-04-04;294(6):L1076-84. 2008-04-04 [PMID: 18390828] (Human)
• Breland UM, Halvorsen B, Hol J, ØIe E, Paulsson-Berne G, Yndestad A, Smith C, Otterdal K, Hedin U, Waehre T, Sandberg WJ, Froland SS, Haraldsen G, Gullestad L, Damas JK, Hansson GK, Aukrust P A potential role of the CXC chemokine GROalpha in atherosclerosis and plaque destabilization: downregulatory effects of statins. Arterioscler. Thromb. Vasc. Biol., 2008-02-14;28(5):1005-11. 2008-02-14 [PMID: 18276907] (Human)
• Mayr FB, Spiel AO, Leitner JM, Firbas C, Kliegel T, Jilma-Stohlawetz P, Derendorf H, Jilma B Duffy antigen modifies the chemokine response in human endotoxemia. Crit. Care Med., 2008-01-01;36(1):159-65. 2008-01-01 [PMID: 18007272] (Human)
• Matsuda A, Fukuda S, Matsumoto K, Saito H Th1/Th2 cytokines reciprocally regulate in vitro pulmonary angiogenesis via CXC chemokine synthesis. Am. J. Respir. Cell Mol. Biol., 2007-08-20;38(2):168-75. 2007-08-20 [PMID: 17709600] (Human)
• Neuhoff S, Moers J, Rieks M, Grunwald T, Jensen A, Dermietzel R, Meier C Proliferation, differentiation, and cytokine secretion of human umbilical cord blood-derived mononuclear cells in vitro. Exp. Hematol., 2007-07-01;35(7):1119-31. 2007-07-01 [PMID: 17588481] (Human)
• Tsai WH, Hsu HC, Lin CC, Ho CK, Kou YR Role of interleukin-8 and growth-regulated oncogene-alpha in the chemotactic migration of all-trans retinoic acid-treated promyelocytic leukemic cells toward alveolar epithelial cells. Crit. Care Med., 2007-03-01;35(3):879-85. 2007-03-01 [PMID: 17235257] (Human)
• Yu JJ, Ruddy MJ, Wong GC, Sfintescu C, Baker PJ, Smith JB, Evans RT, Gaffen SL An essential role for IL-17 in preventing pathogen-initiated bone destruction: recruitment of neutrophils to inflamed bone requires IL-17 receptor-dependent signals. Blood, 2007-01-03;109(9):3794-802. 2007-01-03 [PMID: 17202320] (Mouse)
• Antoniou KM, Tzouvelekis A, Alexandrakis MG, Sfiridaki K, Tsiligianni I, Rachiotis G, Tzanakis N, Bouros D, Milic-Emili J, Siafakas NM Different angiogenic activity in pulmonary sarcoidosis and idiopathic pulmonary fibrosis. Chest, 2006-10-01;130(4):982-8. 2006-10-01 [PMID: 17035428] (Human)
• Abe Y, Fornage M, Yang CY, Bui-Thanh NA, Wise V, Chen HH, Rangaraj G, Ballantyne CM L5, the most electronegative subfraction of plasma LDL, induces endothelial vascular cell adhesion molecule 1 and CXC chemokines, which mediate mononuclear leukocyte adhesion. Atherosclerosis, 2006-10-04;192(1):56-66. 2006-10-04 [PMID: 17022986] (Human)
• Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis. J. Immunol., 2005-11-01;175(9):6177-89. 2005-11-01 [PMID: 16237115] (Human)
• Ko FW, Lau CY, Leung TF, Wong GW, Lam CW, Hui DS Exhaled breath condensate levels of 8-isoprostane, growth related oncogene alpha and monocyte chemoattractant protein-1 in patients with chronic obstructive pulmonary disease. Respir Med, 2005-10-06;100(4):630-8. 2005-10-06 [PMID: 16213701] (Human)
• Maheshwari A, Christensen RD, Calhoun DA ELR+ CXC chemokines in human milk. Cytokine, 2003-11-07;24(3):91-102. 2003-11-07 [PMID: 14581003] (Human)
• Ciesielski CJ, Andreakos E, Foxwell BM, Feldmann M TNFalpha-induced macrophage chemokine secretion is more dependent on NF-kappaB expression than lipopolysaccharides-induced macrophage chemokine secretion. Eur. J. Immunol., 2002-07-01;32(7):2037-45. 2002-07-01 [PMID: 12115625] (Human)
• Hurst SM, Wilkinson TS, McLoughlin RM, Jones S, Horiuchi S, Yamamoto N, Rose-John S, Fuller GM, Topley N, Jones SA Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation. Immunity, 2001-06-01;14(6):705-14. 2001-06-01 [PMID: 11420041] (Human)

FAQs for CXCL1/GRO alpha/KC/CINC-1 (DGR00B). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.