Human Butyrylcholinesterase/BCHE Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Butyrylcholinesterase/BCHE Quantikine ELISA Kit Summary

• Background: The Quantikine Human Butyrylcholinesterase/BCHE Immunoassay is a 4.5 hour solid phase ELISA designed to measure BCHE levels in cell culture supernates, cell lysates, tissue lysates, serum, plasma, saliva, urine, and human milk. It contains CHO cell-expressed recombinant human BCHE and antibodies raised against the recombinant protein. Results obtained for naturally occurring human BCHE showe...d linear curves that were parallel to the standard curves obtained using the Quantikine Human BCHE Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human BCHE.
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• Specificity: Natural and recombinant human BCHE.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: BCHE

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Butyrylcholinesterase/BCHE Quantikine ELISA Kit

• Acylcholine acylhydrolase
• BCHE
• Butyrylcholine esterase
• Butyrylcholinesterase
• CHE1
• CHE1cholinesterase
• Choline esterase II
• cholinesterase 1
• E1
• EC 3.1.1.8
• Pseudocholinesterase

Background

Butyrylcholinesterase (BCHE) is a major acetylcholine hydrolyzing enzyme in the circulation. Although it is present in significant amounts (~3 mg/L) in human plasma, no endogenous physiological substrate has been described for this enzyme. It can degrade a large number of ester-containing compounds in addition to acylcholines. Thus, it is likely to play significant pharmacological and toxicological roles. It is thought to be involved in the pathological process of Alzheimer's disease (AD) by depleting acetylcholine. In contrast to ACHE, it attenuates amyloid fibril formation in vitro. BCHE inhibitors have been used to delay symptoms of AD patients by virtue of their ability to enhance acetylcholine availability. Its involvement in a cholinergic anti-inflammatory pathway connect BCHE and ACHE with a possible marker of low-grade systemic inflammation observed in Type-2 diabetes, obesity, hypertension, coronary heart disease, and AD. BCHE can exist in monomeric and multimeric forms. The expressed recombinant mouse BCHE contains multiple forms that consist of soluble monomers, dimers, and tetramers.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Butyrylcholinesterase/BCHE (DBCHE0)(3)

Publications using DBCHE0

• G Li, Y Tataka, K Kamemoto, H Wen, K Yamanaka, C Nagayama, M Miyashita Does butyrylcholinesterase mediate exercise-induced and meal-induced suppression in acylated ghrelin? Endocrine journal, 2022-09-01;0(0):. 2022-09-01 [PMID: 36047188] (Human)
• V Gupta, CL Cadieux, D McMenamin, CA Medina-Jas, M Arif, O Ahonkhai, E Wielechows, M Taheri, Y Che, T Goode, MP Limberis, M Li, DM Cerasoli, AP Tretiakova, JM Wilson Adeno-associated virus-mediated expression of human butyrylcholinesterase to treat organophosphate poisoning PLoS ONE, 2019-11-25;14(11):e0225188. 2019-11-25 [PMID: 31765413] (Transgenic Mouse)
• YC Chen, WH Chou, CP Fang, TH Liu, HH Tsou, Y Wang, YL Liu Serum Level and Activity of Butylcholinesterase: A Biomarker for Post-Stroke Dementia J Clin Med, 2019-10-24;8(11):. 2019-10-24 [PMID: 31653081] (Human)

Bioinformatics

• Gene Symbol: BCHE