Human BAFF/BLyS/TNFSF13B DuoSet ELISA, 5 Plate

• Reactivity: Hu
• Applications: ELISA
• Conjugate: Biotin

Human BAFF/BLyS/TNFSF13B DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: TNFSF13B

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human BAFF/BLyS/TNFSF13B DuoSet ELISA, 5 Plate

• ApoL related ligand TALL-1
• B lymphocyte stimulator
• BAFF
• BAFFB-cell activating factor
• B-cell-activating factor
• BLyS
• BLYSB-lymphocyte stimulator
• CD257 antigen
• CD257
• Dendritic cell-derived TNF-like molecule
• DTL
• TALL1
• TALL-1delta BAFF
• TALL1Delta4 BAFF
• THANK
• TNF- and APOL-related leukocyte expressed ligand 1
• TNF and ApoL-related leukocyte expressed ligand 1
• TNF homolog that activates apoptosis
• TNFSF13B
• TNFSF20
• tumor necrosis factor (ligand) superfamily, member 13b
• tumor necrosis factor (ligand) superfamily, member 20
• tumor necrosis factor ligand superfamily member 13B
• tumor necrosis factor superfamily, member 13b
• tumor necrosis factor-like protein ZTNF4
• ZTNF4

Background

BAFF (B-cell activating factor), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a TNF superfamily ligand that plays a critical role in the development and survival of B lineage cells. It can be expressed as a homotrimer or as a heteromer in association with the related protein APRIL. It is produced by many hematopoietic cell types, and a soluble form can be released by activated neutrophils. Both BAFF and APRIL signal through the receptors BCMA and TACI, and BAFF additionally signals through BAFF R. Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity. In addition, BAFF costimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells.

Publications for BAFF/BLyS/TNFSF13B (DY124-05)(16)

Publications using DY124-05

• Brunner, M;Guggisberg, D;Sprecher, M;Pastva, O;Bürki, K;Houtman, M;Kreuzer, M;Krättli, SA;Jahnke, L;Roceri, M;Bruggmann, R;Elhai, M;Maurer, B;Marti, TM;Ospelt, C;Klein, K; Pro-Inflammatory Properties of Salivary Gland-Derived Fibroblasts-Implications in Sjögren's Disease Cells 2025-04-08 [PMID: 40277884] (Human)
• Courtemanche, O;Huppé, CA;Blais-Lecours, P;Maranda, C;Morissette, MC;Blanchet, MR;Dion, G;Marsolais, D; Ex Vivo Overactivation of Lymphocyte Subsets in Fibrotic Hypersensitivity Pneumonitis Is Blunted by a Sphingosine-1-Phosphate Receptor Ligand International journal of molecular sciences 2025-03-29 [PMID: 40243992] (Human)
• Cencioni, MT;Magliozzi, R;Palmisano, I;Suwan, K;Mensi, A;Fuentes-Font, L;Villar, LM;Fernández-Velasco, JI;Migallón, NV;Costa-Frossard, L;Monreal, E;Ali, R;Romozzi, M;Mazarakis, N;Reynolds, R;Nicholas, R;Muraro, PA; Soluble CD27 is an intrathecal biomarker of T-cell-mediated lesion activity in multiple sclerosis Journal of neuroinflammation 2024-04-12 [PMID: 38609999] (Human)
• de Jong, MME;Fokkema, C;Papazian, N;Czeti, Á;Appelman, MK;Vermeulen, M;van Heusden, T;Hoogenboezem, RM;van Beek, G;Tahri, S;Sanders, MA;van de Woestijne, PC;Gay, F;Moreau, P;Büttner-Herold, M;Bruns, H;van Duin, M;Broijl, A;Sonneveld, P;Cupedo, T; An IL-1?-driven neutrophil-stromal cell axis fosters a BAFF-rich protumor microenvironment in individuals with multiple myeloma Nature immunology 2024-04-10 [PMID: 38600356]
• W Alturaiki, H Alkadi, S Alamri, ME Awadalla, A Alfaez, A Mubarak, MA Alanazi, FQ Alenzi, BF Flanagan, B Alosaimi Association between the expression of Toll-like receptors, cytokines, and homeostatic chemokines in SARS-CoV-2 infection and COVID-19 severity Heliyon, 2022-12-24;0(0):e12653. 2022-12-24 [PMID: 36589720] (Human)
• L Bolkun, M Tynecka, T Wasiluk, J Piszcz, A Starosz, K Grubczak, M Moniuszko, A Eljaszewic A Proliferation-Inducing Ligand and B-Cell Activating Factor Are Upregulated in Patients with Essential Thrombocythemia Journal of Clinical Medicine, 2022-08-09;11(16):. 2022-08-09 [PMID: 36012902] (Human)
• S Piantoni, F Regola, S Masneri, M Merletti, T Lowin, P Airò, A Tincani, F Franceschi, L Andreoli, G Pongratz Characterization of B- and T-Cell Compartment and B-Cell Related Factors Belonging to the TNF/TNFR Superfamily in Patients With Clinically Active Systemic Lupus Erythematosus: Baseline BAFF Serum Levels Are the Strongest Predictor of Response to Belimumab after Twelve Months of Therapy Frontiers in Pharmacology, 2021-05-21;12(0):666971. 2021-05-21 [PMID: 34093196] (Human)
• F Basta, T Möckel, J Petersohn, M Meineck, K Triantafyl, S Engel, A Weinmann, F Luessi, J Weinmann-M, A Schwarting The relationship between BAFF serum levels, anti-NMDAR autoantibodies and fatigue in patients with systemic lupus erythematosus and multiple sclerosis Autoimmunity reviews, 2021-03-14;20(5):102802. 2021-03-14 [PMID: 33727153] (Human)
• LL Zhang, H Xiao, F Zhang, YJ Wu, JL Shu, Y Li, Y Tai, SQ Xu, JH Xu, W Wei BAFF, involved in B cell activation through the NF-&kappaB pathway, is related to disease activity and bone destruction in rheumatoid arthritis Acta pharmacologica Sinica, 2021-01-22;0(0):. 2021-01-22 [PMID: 33483588] (Human)
• K Lashkari, GC Teague, U Beattie, J Betts, S Kumar, MM McLaughlin, FJ López Plasma biomarkers of the amyloid pathway are associated with geographic atrophy secondary to age-related macular degeneration PLoS ONE, 2020-08-07;15(8):e0236283. 2020-08-07 [PMID: 32764794] (Human)
• Cohen A, Garfall A, Stadtmauer E, Melenhorst J, Lacey S, Lancaster E, Vogl D, Weiss B, Dengel K, Nelson A, Plesa G, Chen F, Davis M, Hwang W, Young R, Brogdon J, Isaacs R, Pruteanu-Malinici I, Siegel D, Levine B, June C, Milone M B cell maturation antigen-specific CAR T cells are clinically active in multiple myeloma. J Clin Invest, 2019-03-21;130(0):. 2019-03-21 [PMID: 30896447] (Human)
• GR Kathamuthu, K Moideen, VV Banurekha, D Nair, R Sridhar, D Baskaran, S Babu Altered circulating levels of B cell growth factors and their modulation upon anti-tuberculosis treatment in pulmonary tuberculosis and tuberculous lymphadenitis PLoS ONE, 2018-11-14;13(11):e0207404. 2018-11-14 [PMID: 30427928] (Human)
• AC Lundell, I Nordstr”m, K Andersson, C Lundqvist, E Telemo, S Nava, H Kaipe, A Rudin IFN type I and II induce BAFF secretion from human decidual stromal cells Sci Rep, 2017-01-06;7(0):39904. 2017-01-06 [PMID: 28057926] (Human)
• Ramnath Misra Urinary B cell Activating Factor (BAFF) and A Proliferation Inducing Ligand (APRIL): Potential biomarkers of Active Lupus Nephritis Clin. Exp. Immunol., 2016-12-05;0(0):. 2016-12-05 [PMID: 27804111] (Human)
• Utility of CSF Cytokine/Chemokines as Markers of Active Intrathecal Inflammation: Comparison of Demyelinating, Anti-NMDAR and Enteroviral Encephalitis PLoS ONE, 2016-08-30;11(8):e0161656. 2016-08-30 [PMID: 27575749] (Human)
• Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells. Leukemia, 2015-07-03;30(1):163-72. 2015-07-03 [PMID: 26139429] (Human)

FAQs for BAFF/BLyS/TNFSF13B (DY124-05). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TNFSF13B