HSP27 Antibody

Images

 
Western Blot: HSP27 Antibody [NBP2-16891] - Non-transfected (-) and transfected (+) HT-29 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody.
Immunohistochemistry-Paraffin: Hsp27 Antibody [NBP2-16891] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.
Western Blot: Hsp27 Antibody [NBP2-16891] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 12% SDS PAGE gel, diluted at 1:1000.
Western Blot: HSP27 Antibody [NBP2-16891] - Wild-type (WT) and HSP27 knownout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 antibody. The HRP-conjugated ...read more
Immunoprecipitation: Hsp27 Antibody [NBP2-16891] - Sample: 1000 ug HeLa whole cell lysate/extract A. 40 ug HeLa whole cell lysate/extract B. Control with 2. 5 ug of preimmune rabbit IgG C. Immunoprecipitation of HSP27 ...read more

Product Details

Summary
Reactivity Hu, MkSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.82 mg/ml
     

Genetic Strategies

   

Order Details

HSP27 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human HSP27. The exact sequence is proprietary.
Localization
Cytoplasm; Nucleus; Spindle
Predicted Species
Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HSPB1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:500-1:1000
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.82 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HSP27 Antibody

  • 28 kDa heat shock protein
  • DKFZp586P1322
  • Estrogen-regulated 24 kDa protein
  • Heat shock 27 kDa protein
  • heat shock 27kD protein 1
  • heat shock 27kDa protein 1
  • heat shock protein beta-1
  • HMN2B
  • HS.76067
  • HSP25
  • HSP27
  • HSP27HSP 27
  • HSP28CMT2F
  • HSPB1
  • SRP27
  • Stress-responsive protein 27

Background

The protein encoded by this gene is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN). [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSP27 Antibody (NBP2-16891) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol HSPB1
Entrez
Uniprot