HSP20/HSPB6 Antibody (494310) [Unconjugated]

Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat HSP20/HSPB6 Monoclonal Antibody (Catalog # MAB4200) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSP20/HSPB6 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western shows lysates of human heart tissue, loaded at 0.5 mg/ml. A specific band was detected for HSP20/HSPB6 at approximately 26 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat HSP20/HSPB6 Monoclonal Antibody (Catalog # MAB4200). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western
• Clone: 494310
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

HSP20/HSPB6 Antibody (494310) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human HSP20/HSPB6
  Met1-Lys160
  Accession # O14558
• Specificity: Detects human, mouse, and rat HSP20/HSPB6 in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSPB6
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS, NaCl and Tween® 20 with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HSP20/HSPB6 Antibody (494310) [Unconjugated]

• FLJ32389
• Heat shock 20 kDa-like protein p20
• heat shock protein beta-6
• heat shock protein, alpha-crystallin-related, B6
• HSP20
• HSPB6

Background

Heat shock protein 20 kDa (HSP20), also known as heat shock protein, alpha-crystallin-related, B6 (HSPB6), is a 160 amino acid, 20 kDa member of the small heat shock protein family, which also includes HSP27, HSP25, and the alpha -crystallins. Heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. HSPs are ubiquitously expressed in all organisms. Many HSPs are induced in response to various types of environmental stresses like heat, cold, and oxygen deprivation. Although HSP20 expression does not increase in response to heat stress, HSP20 does translocate from the cytosol to the nucleus during heat stress. The highest levels of HSP20 are found in skeletal, smooth and cardiac muscle. HSP20 may also be secreted into plasma. Human HSP20 shares 88% and 90% amino acid identity with mouse and rat HSP20, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSP20/HSPB6 Antibody (MAB4200)(1)

Publication using MAB4200

• E Adriaensse, B Asselbergh, P Rivera-Mej, S Bervoets, L Vendredy, V De Winter, K Spaas, R de Rycke, G van Isterd, F Impens, T Langer, V Timmerman Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space Nature Cell Biology, 2023-01-23;0(0):. 2023-01-23 [PMID: 36690850] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: HSPB6
• Uniprot:
  • O14558(Human)