HSP10/EPF Antibody (M1.2)

Images

 
Western Blot: Hsp10 Antibody (M1.2) [NB120-13527] - Chlamydia-infected Human HeLa cell lysate.

Product Details

Summary
Reactivity BaSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clone
M1.2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.92 mg/ml

HSP10/EPF Antibody (M1.2) Summary

Immunogen
A 16 residue synthetic MAP peptide KKDDKGQQLPFEVQVG based on C. trachomatis cpn10 (residues 54-69).
Localization
Mitochondrial
Specificity
This affinity purified detects a 15 kDa protein, corresponding to the apparent molecular mass of cpn10 on SDS-PAGE immunoblots, in C. trachomatis infected cells. This will not react with the human or mouse cpn10 homolog nor with E. coli GroES.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
HSPE1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.25 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
Application Notes
This antibody is useful in ELISA, Immunocytochemistry, Immunoprecipitation and detects a band of ~15kDa by Western blot.

Reactivity Notes

Reacts with C. trachomatis. Does not cross-react with Human, Mouse or E. coli.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Liquid. In PBS containing 0.1mM PMSF.
Preservative
No Preservative
Concentration
0.92 mg/ml
Purity
Protein G purified

Alternate Names for HSP10/EPF Antibody (M1.2)

  • 10 kDa chaperonin
  • 10 kDa heat shock protein, mitochondrial
  • Chaperonin 10 Homolog
  • Chaperonin 10
  • CPN10HSP10
  • Early-pregnancy factor
  • EPF
  • GroES
  • heat shock 10kD protein 1 (chaperonin 10)
  • heat shock 10kDa protein 1 (chaperonin 10)
  • HSP10
  • HSPE1

Background

Chaperonin 10 (Cpn10 or Hsp10), is the ~10 kDa mammalian equivalent to GroES of E. coli. Chaperonins play fundamental roles in the folding, assembly, and translocation of other proteins. Chaperonin 10 has been located in human platelets but more importantly it may also be present in human maternal serum. It has been reported that human Cpn10 is identical with Early pregnancy factor (EPF), which is involved in control over cell growth and development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HSP10/EPF Antibody (NB120-13527) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB120-13527AF405
Alexa Fluor 488 Labeled NB120-13527AF488
Alexa Fluor 647 Labeled NB120-13527AF647
Alexa Fluor 700 Labeled NB120-13527AF700
DyLight 350 Labeled NB120-13527UV
DyLight 405 Labeled NB120-13527V
DyLight 488 Labeled NB120-13527G
DyLight 680 Labeled NB120-13527FR
DyLight 755 Labeled NB120-13527IR

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Bioinformatics

Gene Symbol HSPE1
Entrez