HSP10/EPF Antibody (M1.2)

Western Blot: Hsp10 Antibody (M1.2) [NB120-13527] - Chlamydia-infected Human HeLa cell lysate.

Product Discontinued

HSP10/EPF Antibody (M1.2) Summary

• Immunogen: A 16 residue synthetic MAP peptide KKDDKGQQLPFEVQVG based on C. trachomatis cpn10 (residues 54-69).
• Localization: Mitochondrial
• Specificity: This affinity purified detects a 15 kDa protein, corresponding to the apparent molecular mass of cpn10 on SDS-PAGE immunoblots, in C. trachomatis infected cells. This will not react with the human or mouse cpn10 homolog nor with E. coli GroES.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSPE1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 0.25 ug/ml
• Application Notes: This antibody is useful in ELISA, Immunocytochemistry, Immunoprecipitation and detects a band of ~15kDa by Western blot.

Reactivity Notes

Reacts with C. trachomatis. Does not cross-react with Human, Mouse or E. coli.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Liquid. In PBS containing 0.1mM PMSF.
• Preservative: No Preservative
• Concentration: 0.92 mg/ml
• Purity: Protein G purified

Alternate Names for HSP10/EPF Antibody (M1.2)

• 10 kDa chaperonin
• 10 kDa heat shock protein, mitochondrial
• Chaperonin 10 Homolog
• Chaperonin 10
• CPN10HSP10
• Early-pregnancy factor
• EPF
• GroES
• heat shock 10kD protein 1 (chaperonin 10)
• heat shock 10kDa protein 1 (chaperonin 10)
• HSP10
• HSPE1

Background

Chaperonin 10 (Cpn10 or Hsp10), is the ~10 kDa mammalian equivalent to GroES of E. coli. Chaperonins play fundamental roles in the folding, assembly, and translocation of other proteins. Chaperonin 10 has been located in human platelets but more importantly it may also be present in human maternal serum. It has been reported that human Cpn10 is identical with Early pregnancy factor (EPF), which is involved in control over cell growth and development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSPE1