HSD17B8 Overexpression Lysate (Native)

Images

 
Western Blot: HSD17B8 Overexpression Lysate (Adult Normal) [NBL1-11731] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HSD17B8.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

HSD17B8 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203806 and based on accession number NM_014234. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens hydroxysteroid (17-beta) dehydrogenase 8 (HSD17B8), mRNA.
Gene
HSD17B8

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
26.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for HSD17B8

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for HSD17B8 Overexpression Lysate (Native)

  • D6S2245EdJ1033B10.9
  • EC 1.1.1.-
  • EC 1.1.1.62
  • EC 1.1.1.63,17-beta-HSD 8
  • estrogen 17-oxidoreductase
  • FABGLestradiol 17-beta-dehydrogenase 8
  • H2-KE6
  • HKE6FabG (beta-ketoacyl-[acyl-carrier-protein] reductase, E coli) like (E. coli)
  • hydroxysteroid (17-beta) dehydrogenase 8,3-oxoacyl-[acyl-carrier-protein] reductase
  • KE6
  • ke-6
  • Ke-6,17-beta-hydroxysteroid dehydrogenase 8
  • Protein Ke6
  • Really interesting new gene 2 protein
  • RING2FABG
  • SDR30C1
  • short chain dehydrogenase/reductase family 30C, member 1
  • Testosterone 17-beta-dehydrogenase 8

Background

In mice, the Ke6 protein is a 17-beta-hydroxysteroid dehydrogenase that can regulate the concentration of biologically active estrogens and androgens. It is preferentially an oxidative enzyme and inactivates estradiol, testosterone, and dihydrotestosterone. However, the enzyme has some reductive activity and can synthesize estradiol from estrone. The protein encoded by this gene is similar to Ke6 and is a member of the short-chain dehydrogenase superfamily. An alternatively spliced transcript of this gene has been detected, but the full-length nature of this variant has not been determined. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for HSD17B8 Lysate (NBL1-11731) (0)

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Bioinformatics

Gene Symbol HSD17B8