Recombinant Human HR6B/UBE2B His (N-Term) Protein

SDS-Page: Human HR6B/UBE2B His (N-Term) Protein [NBP2-35088]

• Reactivity: Hu
• Applications: PAGE
• Format: Carrier-Free

Recombinant Human HR6B/UBE2B His (N-Term) Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 152 amino acids corresponding to HR6B/UBE2B and 14 amion acid vector sequence including 6x His tag at N-terminusSource: E. coli

  Amino Acid Sequence: MHHHHHHAMG QLRSMSTPAR RRLMRDFKRL QEDPPVGVSG APSENNIMQW NAVIFGPEGT PFEDGTFKLV IEFSEEYPNK PPTVRFLSKM FHPNVYADGS ICLDILQNRW SPTYDVSSIL TSIQSLLDEP NPNSPANSQA AQLYQENKRE YEKRVSAIVE QSWNDS

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: UBE2B
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of HR6B/UBE2B as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 19.0 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 7.6 with 125 mM NaCl, 10 % Glycerol, 1 mM DTT.
• Preservative: 5% Trehalose
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Please contact technical support for detailed reconstitution instructions.

Alternate Names for Recombinant Human HR6B/UBE2B His (N-Term) Protein

• E2 protein
• E2-17k
• E2-17kDa
• HHR6B
• HR6B
• RAD6 homolog B
• RAD6BEC 6.3.2.19
• UBC2
• UBE2B
• Ubiquitin carrier protein B
• ubiquitin-conjugating enzyme E2 B
• Ubiquitin-conjugating enzyme E2-17 kDa
• ubiquitin-conjugating enzyme E2B (RAD6 homolog)
• Ubiquitin-protein ligase B

Background

This E2 enzyme encodes for the human homolog of the yeast DNA repair gene RAD6, which is induced by DNA damaging agents. UBE2B can conjugate ubiquitin to histone H2A in an E3- independent manner in vitro, and is essential for the multiubiquitination and degradation of N-end rule substrates. Additionally, UBE2B may have a role in sepsis-induced muscle protein proteolysis and cancer-induced cachexia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: UBE2B